116 research outputs found

    SOME POSSIBILITIES OF VEGETATIVE PROPAGATION OF STRAWBERRY CULTIVARS IN VITRO

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    This work presents the production technology of five strawberry cultivars by micropropagation. Starting materials were taken in May or June from the stolons that were in the air and meristems of 0.5mm were isolated in the laboratory. The isolated strawberry meristems were cultivated in a medium containing macro and micro elements and phytohormones in different concentrations. The highest percentage of initiated culture was recorded by the cultivar Senga Sengana (81.48%) and the lowest by the cultivar Marmolada (48.00%). Optimal hormone balance for initiating culture strawberries was 1.0 mg / l BAP (Benzylaminopurine), 0,1 mg / l IBA (indole-3 -butyric acid) and 0.1 mg / l GA3 (gibberellic acid). After 50 days of cultivation in the culture, the formed shoots were transferred to medium for multiplication. The multiplication of isolated buds was achieved on the medium with phytohormones BAP and IBA. The highest multiplication index was recorded by the cultivar Senga Sengana (8.77) and the lowest by the cultivar Marmolada (5.42). When planlets reached a height of 10-12mm they were transferred to the rooting medium. The optimal concentration of phytohormones IBA for rooting of the strawberry cultivars was about 5 mg / l. Number of roots ranged from 5.09 for the cultivar Idea to 6.11 for the cultivar Senga Sengana. After two months the plants with developed roots were transplanted into peat briquettes. Adjustment by external conditions was conducted in greenhouses under a mist system. The reception was submitted well by the planted strawberries (90%). The results presented in this paper indicate that micropropagation can speed up the process of getting healthy and HIGH quality planting material ofstrawberries. The procedure for obtaining strawberries by micropropagation can be accelerated by proper selection of the combination and concentrations of phytohormones

    THE INFLUENCE OF THE ANTHROPOGENIC FACTOR ON THE BIODIVERSITY OF CODLING MOTH NATURAL ENEMIES

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    The human role in bioecological examinations of codling moths is of crucial importance for the integral protection of pome fruit and the production of healthy and safe food. The codling moth (Cydia pomonella L.) is one of the most significant apple pests in terms of causing the diminished yield and poorer fruit quality in apples. As such, the codling moth requires daily monitoring by agricultural producers. The aim of this research was defined by the need for establishing the presence of codling moth natural enemies and the estimation of their codependence in apple plantations with various methods of protection. The examinations were carried out on the territory of Southern Serbia from 2005 to 2008. During the research, the standard entomological methods, such as pheromone traps, branch beating and placing bands around tree trunks, were used to track the presence, number and population dynamics of the codling moth and its natural enemies. The research results revealed the dominant role that the anthropogenic factor has in many segments of the integral apple protection and the production of high-quality apple fruit. Faunistic researches are inevitable for the correct determination of human actions, due to the clear composition of autochthonous natural enemies of the codling moth. The natural enemies of the codling moth whose presence was determined during the research belong to the following orders: Hymenoptera, Diptera, Dermaptera, Neuroptera, Coleoptera and Heteroptera. The analysis that has examined the human influence on the autochthonous natural enemies of the codling moth also contributes to the preservation of biodiversity and agrobiocenosis, as well as to the production of high-quality, safe food

    A diagrammatic presentation of the category 3Cob

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    A category equivalent to the category of 3-dimensional cobordisms is defined in terms of planar diagrams. The operation of composition in this category is completely described via these diagrams.Comment: 26 page

    Pomološke karakteristike klonova Oblačinske višnje u leskovačkom području

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    This work presents a three-year study of oblacinska sour cherry clones. The studies were carried out at the production plantings of sour cherry of DP '' Porecje'' Vucje laboratories and the Faculty of Technology. The work presents the technology of nine clones of oblacinska sour cherry. Clone 28, which is the most prevalent in productive plantings, was used for comparison. During the performance of experiment, the vegetative and generative potential, important chemical and technological properties of fruits, yield, the content of dry matter and sugar were monitored. The obtained data of oblacinska sour cherries clones were processed by using the statistical methods. Based on all the examined traits, the following three sour cherry clones showed the highest quality properties: 56/21, 34/10 and 62/22.U radu su prikazana trogodišnja istraživanja devet klonova Oblačinske višnje. Ispitivanja su obavljena u proizvodnim zasadima Oblačinske višnje u D.P. „Porečje“ Vučje i laboratorijama Tehnološkog fakulteta. Za upoređenje je korišćen klon 28,koji je najviše rasprostranjen u proizvodnim zasadima. Tokom istraživanja praćen je vegetativni i generativni potencijal, fenologija cvetanja, važnije hemijske i tehnološke osobine plodova, sadržaj rastvorljivih suvih materija i šećera. Dobijeni podaci ispitivanih klonova Oblačinske višnje obrađeni su statističkim metodama. Na osnovu istraživanja najbolje rezultate su pokazala tri klona Oblačinske višnje i to: 56/21, 34/10 i 62/22

    Forenzički aspekti postmortalne analize transferina s nedostatkom ugljenih hidrata kao markera zloupotrebe alkohola

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    Introduction Carbohydrate-deficient transferrin (CDT) has been suggested as one of alcohol abuse indicators having produced good results in forensic medicine for years. Objective The aim of the study was to identify correlation between present methodology of alcohol abuse diagnosis at autopsy (macroscopic and microscopic findings) and CDT examination using the method of isoelectrofocusing (IEF) in polyacrylamide gel electrophoresis (PAGE). We also analyzed if the time interval between the moment of death and blood sample collection influences CDT findings. Methods The method used for CDT analysis was IEF-PAGE. Sera of 49 males and 11 females aged 14-87 years, average age 46.85 +/- 18.53, were used in this study. Control group consisted of five patients who died after medical treatment that lasted longer than 15 days, and five patients who started Disulfiram therapy in controlled hospital environment. Results The results obtained in CDT examination in dead bodies' sera showed sensitivity 59% and specificity 71%. A high incidence of falsely positive CDT result was noticed in liver failure and cirrhosis of non-alcoholic origin. CDT analysis is also possible to be done in samples collected postmortem up to 76 hours. Conclusion In forensic medicine, the method of CDT determination is reliable for the diagnosis of alcohol abuse.Uvod. Poslednjih godina transferin s nedostatkom ugljenih hidrata (engl. carbohydrate-deficient transferrin - CDT) jedan je od markera zloupotrebe alkohola koji je pokazao najbolje rezultate u sudskoj medicini. Cilj rada. Cilj studije je bio da se odredi korelacija između aktuelne metodologije dijagnoze zloupotrebe alkohola na postmortalnom materijalu (makroskopski i mikroskopski nalaz) i određivanja CDT korišćenjem metode isoelektrofokusiranja (engl. isoelectric focusing - IEF) u poliakrilamidnom gelu (engl. polyacrylamide gel electrophoresis - PAGE). Utvrđivano je da li interval između vremena smrti i uzimanja uzoraka za CDT analizu utiče na nalaz CDT. Metode rada. Za analizu CDT korišćena je metoda IEF-PAGE. Za studiju su analizirani serumi 49 muškaraca i 11 žena prosečne starosti od 46,85±18,53 godina (raspon 14-87 godina). Kontrolnu grupu činilo je pet pacijenata koji su umrli nakon bolničkog lečenja koje je trajalo duže od 15 dana i pet pacijenata kod kojih je u kontrolisanim bolničkim uslovima počelo lečenje disulfiramom. Rezultati. Dobijeni rezultati pokazuju da ova metoda analize CDT na postmortalnom materijalu ima senzitivnost od 59% i specifičnost od 71%. Visoka učestalost lažno pozitivnih rezultata utvrđena je kod oboljenja jetre i ci- roze nealkoholnog porekla. Analizu CDT je moguće raditi i iz uzoraka uzetih do 76 sati nakon smrti. Zaključak. U sudskomedicinskoj praksi ova metoda analize CDT može se koristiti za dijagnostikovanje hronične zloupotrebe alkohola

    Impact of Selenium Addition to Animal Feeds on Human Selenium Status in Serbia

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    Research conducted during the 1980s demonstrated Se deficiency in humans. Increased inclusion of selenium in animal feeds started from the year 2000 onwards. The aim of this study was to estimate the effects of selenium inclusion in animal feeds on human selenium status and dietary habits of the Serbian population related to food of animal origin. Plasma selenium concentration in healthy adult volunteers, including residents of one of the regions with the lowest (Eastern Serbia, n = 60) and of one of the regions with the highest Se serum levels reported in the past (Belgrade, n = 82), was determined by hydride generation atomic absorption spectrometry. Multivariate analysis was employed to determine the correlation between Se plasma levels and dietary intake data derived from food frequency questionnaires and laboratory tests. The mean plasma Se level of the participants was 84.3 +/- 15.9 g/L (range: 47.3-132.1 g/L), while 46% of participants had plasma Se levels lower than 80 g/L. Frequency of meat, egg, and fish consumption was significantly correlated with plasma selenium level (r = 0.437, p = 0.000). Selenium addition to animal feed in the quantity of 0.14 mg/kg contributed to the improvement of human plasma selenium levels by approximately 30 mu g/L

    THE HIGH FREQUENCY SURFACE WAVE RADAR SOLUTION FOR VESSEL TRACKING BEYOND THE HORIZON

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    With maximum range of about 200 nautical miles (approx. 370 km) High Frequency Surface Wave Radars (HFSWR) provide unique capability for vessel detection far beyond the horizon without utilization of any moving platforms. Such uniqueness requires design principles unlike those usually used in microwave radar. In this paper the key concepts of HFSWR based on Frequency Modulated Continuous (FMCW) principles are presented. The paper further describes operating principles with focus on signal processing techniques used to extract desired data. The signal processing describes range and Doppler processing but focus is given to the Digital Beamforming (DBF) and Constant False Alarm Rate (CFAR) models. In order to better present the design process, data obtained from the HFSWR sites operating in the Gulf of Guinea are used.  
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