Angiotensin-converting enzyme 2 (ACE2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus

Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has identities of 99.3, 89.2, 83.9 and 80.4% to ACE2 proteins from dog, masked palm civet (pcACE2), human (huACE2) and bat, respectively. There are six amino acid changes in rdACE2 compared with huACE2, and four changes compared with pcACE2, within the 18 residues of ACE2 known to make direct contact with the SARS-CoV S protein. A HeLa cell line stably expressing rdACE2 was established; Western blot analyses and an enzyme-activity assay indicated that the cell line expressed ACE2 at a similar level to two previously established cell lines that express ACE2 from human and masked palm civet, respectively. Human immunodeficiency virus-backboned pseudoviruses expressing spike proteins derived from human SARS-CoV or SARS-CoV-like viruses of masked palm civets and raccoon dogs were tested for their entry efficiency into these cell lines. The results showed that rdACE2 is a more efficient receptor for human SARS-CoV, but not for SARS-CoV-like viruses of masked palm civets and raccoon dogs, than huACE2 or pcACE2. This study provides useful data to elucidate the role of raccoon dog in SARS outbreaks. © 2009 SGM.published_or_final_versio

Precision Enhancement of Pavement Roughness Localization with Connected Vehicles

Raj Bridgelall is the program director for the Upper Great Plains Transportation Institute (UGPTI) Center for Surface Mobility Applications & Real-time Simulation environments (SMARTSeSM).Transportation agencies rely on the accurate localization and reporting of roadway anomalies that could pose serious hazards to the traveling public. However, the cost and technical limitations of present methods prevent their scaling to all roadways. Connected vehicles with on-board accelerometers and conventional geospatial position receivers offer an attractive alternative because of their potential to monitor all roadways in real-time. The conventional global positioning system is ubiquitous and essentially free to use but it produces impractically large position errors. This study evaluated the improvement in precision achievable by augmenting the conventional geofence system with a standard speed bump or an existing anomaly at a pre-determined position to establish a reference inertial marker. The speed sensor subsequently generates position tags for the remaining inertial samples by computing their path distances relative to the reference position. The error model and a case study using smartphones to emulate connected vehicles revealed that the precision in localization improves from tens of metres to sub-centimetre levels, and the accuracy of measuring localized roughness more than doubles. The research results demonstrate that transportation agencies will benefit from using the connected vehicle method to achieve precision and accuracy levels that are comparable to existing laser-based inertial profilers.Mountain Plains Consortium (MPC)https://www.ugpti.org/about/staff/viewbio.php?id=7

Helicoverpa armigera nucleopolyhedrovirus occlusion-derived virus-associated protein, HA100, affects oral infectivity in vivo but not virus replication in vitro

ORF100 (ha100) of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) has been reported as one of the unique genes of group II alphabaculoviruses encoding a protein located in the occlusion-derived virus (ODV) envelope and nucleocapsid. The protein consists of 510 aa with a predicted mass of 58.1 kDa and is a homologue of poly(ADP–ribose) glycohydrolase in eukaryotes. Western blot analysis detected a 60 kDa band in HearNPV-infected HzAM1 cells starting at 18 h post-infection. Transient expression of GFP-fused HA100 in HzAM1 cells resulted in cytoplasmic localization of the protein, but after superinfection with HearNPV, GFPfused HA100 was localized in the nucleus. To study the function of HA100 further, an ha100-null virus was constructed using bacmid technology. Viral one-step growth curve analyses showed that the ha100-null virus had similar budded virus production kinetics to that of the parental virus. Electron microscopy revealed that deletion of HA100 did not alter the morphology of ODVs or occlusion bodies (OBs). However, bioassays in larvae showed that the 50% lethal concentration (LC50) value of HA100-null OBs was significantly higher than that of parental OBs; the median lethal time (LT50) of ha100-null OBs was about 24 h later than control virus. These results indicate that HA100 is not essential for virus replication in vitro. However, it significantly affects the oral infectivity of OBs in host insects, suggesting that the association HA100 with the ODV contributes to the infectivity of OBs in vivo

Probabilistic teleportation of unknown two-particle state via POVM

We propose a scheme for probabilistic teleportation of unknown two-particle state with partly entangled four-particle state via POVM. In this scheme the teleportation of unknown two-particle state can be realized with certain probability by performing two Bell state measurements, a proper POVM and a unitary transformation.Comment: 5 pages, no figur

Multiwavelength Observations of one Galaxy in Marano Field

We report the multiwavelength observations of one intermediate redshift (z=0.3884) galaxy in the Marano Field. These data include ISOCAM middle infrared, VLT/FORS2 spectroscopic and photometric data, associated with the ATCA 1.4 GHz radio and ROSAT PSPC X-ray observations from literature. The Spectral Energy Distribution obtained by VLT spectroscopy exhibits its early-type galaxy property, while, in the same time, it has obvious [OIII]5007 emission line. The diagnostic diagram from the optical emission line ratios shows its Seyfert galaxy property. Its infrared-radio relation follows the correlation of sources detected at 15 \mu and radio. It has a high X-ray luminosity of 1.26*10^{43} ergs/s, which is much higher than the general elliptical galaxies s with the similar B band luminosity, and is about 2 orders of magnitude higher than the derived value from the star forming tracer, the FIR luminosity. This means that the X-ray sources of this galaxy are not stellar components, but the AGN is the dominant component.Comment: 6 pages, 1 PS figure and 4 tables. Publication in ChJAA, Suppl., the Special Issue for The Fifth Microquasar Workshop 2004: http://chjaa.bao.ac.cn/, 2005, Vol.5, 335-34