Patomorfološki nalazi u jetri kod eksperimentalnog otrovanja manganom

Die Untersuchungen wurden an 5 Gruppen von Kaninchen durchgeführt. Vier der Gruppen erhielten jeden zweiten Tag subkutan verschiedene Dosen von MnCl2-Lösung. Nach 16-wöchiger Manganbelastung der Tiere wurde eine Gruppe weiterhin 5 Wochen lang unter denselben Zuchtbedingungen und ohne weitere Manganverabreichung gehalten. Die Kontrolltiere erhielten jeden zweiten Tag, ebenfalls subdermal, physiologische NaCl-Lösung. Die in der Leber festgestellten pathomorphologischen Veränderungen entsprechen einer parenchymatösen Entzündung, deren Zeichen am meisten bei jenen Tieren ausgedrückt sind, die die grössten Mangandosen erhielten und bei denen auch die Mangankonzentration in der Leber die höchste war. Nach der Manganabstellung wurden ein signifikanter Abfall der Mangankonzentration in der Leber, eine deutliche Verminderung der Intensität der regressiven Veränderungen und zugleich Wucherung des Bindegewebes in der Leber festgestellt.Istraživanja su provedena na pet grupa kunića. Četiri grupe su svaki drugi dan dobivale supkutano različite doze otopina MnCl2. Nakon 16-sedmičnog davanja mangana jedna je grupa životinja živjela pod posve istim uvjetima i kroz daljnjih pet sedmica, ali nije više dobivala mangana. Kontrolne životinje dobivale su svaki drugi dan, također supkutano, fiziološke otopine natrijeva klorida. Patomorfološke promjene koje su nađene u jetri odgovaraju parenhimatoznoj upali, znakovi koje su bili najjače izraženi kod onih životinja koje su dobivale najveće doze mangana i kod kojih je koncentracija mangana u jetri bila najviša. Nakon prestanka davanja mangana ustanovljen je značajan pad koncentracije mangana u jetri jasno smanjivanje intenziteta regresivnih promjena i istodobno bujanje veziva u jetri

Subnuclear localization, rates and effectiveness of UVC-induced unscheduled DNA synthesis visualized by fluorescence widefield, confocal and super-resolution microscopy

Unscheduled DNA synthesis (UDS) is the final stage of the process of repair of DNA lesions induced by UVC. We detected UDS using a DNA precursor, 5-ethynyl-2′-deoxyuridine (EdU). Using wide-field, confocal and super-resolution fluorescence microscopy and normal human fibroblasts, derived from healthy subjects, we demonstrate that the sub-nuclear pattern of UDS detected via incorporation of EdU is different from that when BrdU is used as DNA precursor. EdU incorporation occurs evenly throughout chromatin, as opposed to just a few small and large repair foci detected by BrdU. We attribute this difference to the fact that BrdU antibody is of much larger size than EdU, and its accessibility to the incorporated precursor requires the presence of denatured sections of DNA. It appears that under the standard conditions of immunocytochemical detection of BrdU only fragments of DNA of various length are being denatured. We argue that, compared with BrdU, the UDS pattern visualized by EdU constitutes a more faithful representation of sub-nuclear distribution of the final stage of nucleotide excision repair induced by UVC. Using the optimized integrated EdU detection procedure we also measured the relative amount of the DNA precursor incorporated by cells during UDS following exposure to various doses of UVC. Also described is the high degree of heterogeneity in terms of the UVC-induced EdU incorporation per cell, presumably reflecting various DNA repair efficiencies or differences in the level of endogenous dT competing with EdU within a population of normal human fibroblasts

dbar/ubar Asymmetry and the Origin of the Nucleon Sea

The Drell-Yan cross section ratios, $\sigma(p+d)/\sigma(p+p)$, measured in Fermilab E866, have led to the first determination of $\bar d(x) / \bar u(x)$, $\bar d(x) - \bar u(x)$, and the integral of $\bar d(x) - \bar u(x)$ for the proton over the range $0.02 \le x \le 0.345$. The E866 results are compared with predictions based on parton distribution functions and various theoretical models. The relationship between the E866 results and the NMC measurement of the Gottfried integral is discussed. The agreement between the E866 results and models employing virtual mesons indicates these non-perturbative processes play an important role in the origin of the $\bar d$, $\bar u$ asymmetry in the nucleon sea.Comment: 5 pages, 3 figures, ReVTe

CGC, QCD Saturation and RHIC data (Kharzeev-Levin-McLerran-Nardi point of view)

This is the talk given at the Workshop:"Focus on Multiplicitioes", Bari, Italy, 17-19 June,2004.. In this talk, we are going to discuss ion-ion and deuteron - nucleus RHIC data and show that they support, if not more, the idea of the new QCD phase: colour glass condensate with saturated parton density. .Comment: 26 pages with 33 figure

Measurement of the Light Antiquark Flavor Asymmetry in the Nucleon Sea

A precise measurement of the ratio of Drell-Yan yields from an 800 GeV/c proton beam incident on hydrogen and deuterium targets is reported. Over 140,000 Drell-Yan muon pairs with dimuon mass M_{mu+ mu-} >= 4.5 GeV/c^2 were recorded. From these data, the ratio of anti-down (dbar) to anti-up (ubar) quark distributions in the proton sea is determined over a wide range in Bjorken-x. A strong x dependence is observed in the ratio dbar/ubar, showing substantial enhancement of dbar with respect to ubar for x<0.2. This result is in fair agreement with recent parton distribution parameterizations of the sea. For x>0.2, the observed dbar/ubar ratio is much nearer unity than given by the parameterizations.Comment: REVTeX, to be published in Phys. Rev. Let

Search for eta-mesic 4He in the dd->3He n pi0 and dd->3He p pi- reactions with the WASA-at-COSY facility

The search for 4He-eta bound states was performed with the WASA-at-COSY facility via the measurement of the excitation function for the dd->3He n pi0 and dd->3He p pi- processes. The beam momentum was varied continuously between 2.127 GeV/c and 2.422 GeV/c, corresponding to the excess energy for the dd->4He eta reaction ranging from Q=-70 MeV to Q=30 MeV. The luminosity was determined based on the dd->3He n reaction and quasi-free proton-proton scattering via dd->pp n_spectator n_spectator reactions. The excitation functions determined independently for the measured reactions do not reveal a structure which could be interpreted as a narrow mesic nucleus. Therefore, the upper limits of the total cross sections for the bound state production and decay in dd->(4He-eta)_bound->3He n pi0 and dd->(4He-eta)_bound->3He p pi- processes were determined taking into account the isospin relation between both the channels considered. The results of the analysis depend on the assumptions of the N* momentum distribution in the anticipated mesic-4He. Assuming as in the previous works, that this is identical with the distribution of nucleons bound with 20 MeV in 4He, we determined that (for the mesic bound state width in the range from 5 MeV to 50 MeV) the upper limits at 90% confidence level are about 3 nb and about 6 nb for npi0 and ppi- channels, respectively. However, based on the recent theoretical findings of the N*(1535) momentum distribution in the N*-3He nucleus bound by 3.6 MeV, we find that the WASA-at-COSY detector acceptance decreases and hence the corresponding upper limits are 5 nb and 10 nb for npi0 and ppi- channels respectively.Comment: This article will be submitted to JHE

Charge Symmetry Breaking in dd->4He{\pi}0 with WASA-at-COSY

Charge symmetry breaking (CSB) observables are a suitable experimental tool to examine effects induced by quark masses on the nuclear level. Previous high precision data from TRIUMF and IUCF are currently used to develop a consistent description of CSB within the framework of chiral perturbation theory. In this work the experimental studies on the reaction dd->4He{\pi}0 have been extended towards higher excess energies in order to provide information on the contribution of p-waves in the final state. For this, an exclusive measurement has been carried out at a beam momentum of p=1.2 GeV/c using the WASA-at-COSY facility. The total cross section amounts to sigma(tot) = (118 +- 18(stat) +- 13(sys) +- 8(ext)) pb and first data on the differential cross section are consistent with s-wave pion production.Comment: 14 pages, 5 figure

Cross section ratio and angular distributions of the reaction p + d -> 3He + eta at 48.8 MeV and 59.8 MeV excess energy

We present new data for angular distributions and on the cross section ratio of the p + d -> 3He + eta reaction at excess energies of Q = 48.8 MeV and Q = 59.8 MeV. The data have been obtained at the WASA-at-COSY experiment (Forschungszentrum J\"ulich) using a proton beam and a deuterium pellet target. While the shape of obtained angular distributions show only a slow variation with the energy, the new results indicate a distinct and unexpected total cross section fluctuation between Q = 20 MeV and Q = 60 MeV, which might indicate the variation of the production mechanism within this energy interval.Comment: 9 pages, 9 figure

ABC Effect and Resonance Structure in the Double-Pionic Fusion to 3^3He

Exclusive and kinematically complete measurements of the double pionic fusion to $^3$He have been performed in the energy region of the so-called ABC effect, which denotes a pronounced low-mass enhancement in the $\pi\pi$-invariant mass spectrum. The experiments were carried out with the WASA detector setup at COSY. Similar to the observations in the basic $pn \to d \pi^0\pi^0$ reaction and in the $dd \to ^4$He$\pi^0\pi^0$ reaction, the data reveal a correlation between the ABC effect and a resonance-like energy dependence in the total cross section. Differential cross sections are well described by the hypothesis of $d^*$ resonance formation during the reaction process in addition to the conventional $t$-channel $\Delta\Delta$ mechanism. The deduced $d^*$ resonance width can be understood from collision broadening due to Fermi motion of the nucleons in initial and final nuclei