Processing of Biphasic Calciumphosphate/Poly-Dl-Lactide-Co- Glycolide Composite Biomaterials through Cold and Hot Pressing

Poster presented at the The Seventh Yugoslav Materials Research Society Conference - YUCOMAT 2005, Herceg Novi, Montenegro, September 12-16, 2005

Varijabilnost proizvodnih osobina izdvojenih linija pčela u zapadnoj Srbiji

Condition for achieving high performance results in beekeeping is focused on the use of high-quality breeding queen bee. Because that a large amount of research are based on the examination of properties of selected lines of bees and choosing the best queen mothers in the aim of selection and improving the production characteristics of honey bees from the territory of Serbia. The amount of bees, brood, honey and pollen was investigated in two spring and one autumn survey and productivity controlled in the pasture of accacia. Line 1 had the greatest amount of brood (1.23, 4.04 and 5.39), while line 3 had the best spring development. The best quality of brood in all three exams had the lines 1 and 3 (3.00, 3.00 and 2.95) as well as the best productivity compared to the average yield of all other three lines. All tested lines were exhibited variability, which provides enough possibility for their further improvement and single out the mother queenbees with best results.U dvogodišnjem istraživanju ispitivano je četiri odabrane linije medonosne pčele na području Zapadne Srbije. Proizvodne karakteristike praćene su prema standardnoj metodologiji. Na osnovu rezultata istraživanja utvrđeno je da je najveću površinu pčela imala linija 1 (u jesenjem i prvom prolećnom pregledu: 2,10 i 3,27 rama), dok je u drugom prolećnom pregledu najviše pčela imala linija 3 (6,19 rama). Utvrđene razlike u površini pčela nisu bile statistički značajne (P>0.01). Najbolji prolećni razvoj legla između prva dva prolećna pregleda utvrđen je u liniji 3 (povećanje za 1,4 rama) i liniji 1 (povećanje za 1,35 rama). Najveću površinu meda i polena imala su društva linije 1. Najbolji kvalitet legla, po pregledima utvrđen je u linijama 1 i 3. Na osnovu rezultata istraživanja, utvrđeno je da su linija 1 i 3 imale najbolje proizvodne karakteristike. Postojeća varijabilnost ispitivanih karakteristika pčela pruža mogućnost daljeg usavršavanja linija i širenja genetskog napretka na području Srbije, pri čemu prednost treba dati umnožavanju matica na bazi biološkog materijala pčela linija 1 i 3

Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows

Lactoferrin (LTF) is a glycoprotein, a member of transferrin gene family which plays an important role in immune mechanisms in the mammary glands of cows. The amount of lactoferrin increases during inflammatory processes and viral infections. The aim of this investigation was to monitor the distribution of lactoferrin gene genotypes and its connection to milk quality and the occurrence of mammary gland diseases in 46 Holstein-Freisian cows of different age (2-7 years) on a farm near Belgrade. DNA was isolated from blood samples, and the polymorphism of lactoferrin gene was deterimined by PCR-RFLP method using the restriction enzyme Eco RI. We found two alelic forms of this gene in cows included in these experiments (A and B) and two genotypes (AA and AB) in a ratio 71.7% to 28.3%. The genotype BB was not found in this sample. In order to determine the degree of differences between genotypes we used discriminant analysis which has shown that there is a statistically significant difference between genotypes AA and with respect to productive parameters. When analyzed separately, the only parameter which differed significantly (p=0.021) between two genotypes was total milk production. Individuals with observed genotypes are most similar for the amount of milk fat (p=0.271). There is no statistically significant difference in the number of somatic cells in milk samples between the examined genotypes. [Projekat Ministarstva nauke Republike Srbije, br. III46002

EKTOPARAZITI RIBA IZ JEZERA GRADINA, BOSNA I HERCEGOVINA

Tokom proljeća, ljeta i jeseni 2015. provedena je ektoparazitološka studija naribama jezera Gradina, u Bosni i Hercegovini. Osnovni cilj studije bio je otkriti kojeektoparazitske vrste parazitiraju na ribama jezera Gradina. Sekundarni cilj bio jeobjasniti sezonsku dinamiku infestiranosti i intenziteta infestiranosti riba tokomistraživanog perioda. U ukupnom ihtiouzorku od 64 jedinke, determinisano je 5 vrstariba: Alburnus alburnus, Rutilus rutilus, Carassius gibelio, Lepomis gibossus i Abramisbrama. U studiji je determinisano i nekoliko vrsta ektoparazita: Trichodina sp.,Ichthyophthirius multifiliis, Chilodonella sp., Apiosoma sp. i dvije jedinke iz grupeMonogenea. Rezultati istraživanja pokazali su da su najveće vrijednosti infestiranostibile tokom proljeća i ljeta vrstama Trichodina sp. i Ichthyophthirius multifiliis dok jeu jesenskom periodu intenzitet infestiranosti imao niže vrijednosti

Veterinarski informacioni menadžment sistem (VIMS) u procesu prijavljivanja i menadžmenta zaraznih bolesti

A prerequisite to the development of an efficient animal health, food safety and traceability management system in the animal food production chain is the implementation of an integrated veterinary informational management system (VIMS) capable for the capture, storage, analysis and retrieval of data and providing the opportunity for the cumulative gathering of the knowledge and capability for its competent interpretation. Such a system will enable collecting appropriate data, including quality management and inspection controls, from all establishments and commodities in the 'from farm to fork' food production chain (farms, holdings, slaughterhouses, laboratories, traders etc.) in a structured, predefined format, and facilitate competent analyses and reporting of such data, as well as the improvement of the existing programs and strategies. The role of information system in animal disease diagnosis, surveillance and notification, control of national and international trade of commodities, food safety management, investigation of diseases, predictive microbiology and quantitative risk assessment is of great importance for the quality of veterinary service. Integral part of the VIMS is animal disease notification system designed according to and in compliance with international requirements, standards and recommendation and able to exchange relevant information with similar information systems. The aim of this contribution is to describe national animal disease notification system which is in place in Serbia as a part of VIMS.Preduslov za uspostavljanje efikasnog sistema menadžmenta zdravstvene zaštite životinja, bezbednosti hrane i sledljivosti u lancu proizvodnje hrane je uvođenje integrisanog veterinarskog informacionog menadžment sistema (VIMS) razvijenog na takav način da omogući prikupljanje, čuvanje, analizu i povlačenje podataka i obezbedi kumulativno skupljanje saznanja i njihovu stručnu interpretaciju. Takav sistem će omogućiti prikupljanje podataka, uključujući one iz upravljanja kvalitetom i u okviru inspekcijskih kontrola, od svih objekata u sistemu proizvodnje hrane 'od farme do viljuške' (farme, gazdinstva, klanice, laboratorije, lica koja se bave prometom i dr.) u strukturirano, unapred definisanom obliku, i obezbediti kompetentnu analizu takvih podataka kao i unapređenje postojećih programa i strategija. Uloga informacionih sistema u dijagnostici, nadzoru i prijavljivanju zaraznih bolesti, kontroli unutrašnjeg i međunarodnog prometa, upravljanju bezbednošću hrane, istraživanju žarišta zaraznih bolesti, prediktivnoj mikrobiologiji i kvantitativnoj analizi rizika je od izuzetnog značaja za kvalitet veterinarske službe. Sastavni deo VIMS-a je sistem za prijavljivanje zaraznih bolesti dizajniran prema i u saglasnosti sa međunarodnim zahtevima, standardima i preporukama i osposobljen da vrši razmenu određenih podatka sa sličnim informacionim sistemima. Cilj ovog rada je da se opiše nacionalni sistem za prijavljivanje zaraznih bolesti koji je u Srbiji u primeni kao deo VIMS-a

Urban honey - the aspects of its safety

To contribute to the development of urban beekeeping, we designed this study to obtain more information about the contamination of urban bee products with toxic metals, polycyclic aromatic hydrocarbons (PAHs), and pesticides. The samples of honey (N=23), pollen (N=13), and floral nectar (N=6) were collected from the experimental stationary apiary of the Belgrade University Faculty of Agriculture located in centre of Zemun (a municipality of the Belgrade metropolitan area) in 2015 and 2016. Metals (Pb, Cd, As, Cu, Zn, Fe, Mn, Ni, Cr, and Hg) were determined with inductively coupled plasma quadrupole mass spectrometry (ICP-QMS). Polycyclic aromatic hydrocarbons (PAHs) were analysed with high-performance liquid chromatography with fluorescence detection (HPLC-FLD). Pesticides were analysed with gas chromatography - mass spectrometry (GC-MS). The honey samples were generally within the European and Serbian regulatory limits. The levels of all the 123 analysed pesticides were below the limit of quantification (LOQ). Regarding PAH levels in honey, the highest content was found for naphthalene. The elevated levels of Hg and Cr and of PAHs in the pollen samples indicated air pollution. Pesticide residues in pollen, however, were below the LOQ. In nectar, metal levels were relatively similar to those in honey. Our results suggest that the investigated urban honey meets the regulatory requirements for metals, PAHs, and pesticides and is therefore safe for consumption

CORRELATION OF MORPHOLOGIC AND PRODUCTION TRAITS OF HONEY BEE COLONIES FROM SERBIA

Abstract: State of mutual or reciprocal relationship between traits is determined by the correlation coefficients. Correlation of morphological and production traits was determined in two generations of honey bee colonies. In the first generation, mother queen bees were studied, and in the second generation, their offspring. The study of one generation lasted two years. Morphological traits were determined in the beginning of the study and production traits were determined in the spring and autumn inspections of every year. In the first generation, the positive and mostly high correlation between morphological traits, and honey yield and hygienic behaviour was determined. Correlation of morphological traits, colony strength and food supplies per colony ranged from highly positive to slightly negative values. It was found that there was a high correlation between some production traits (colony strength in the spring and autumn inspections and honey yield per colony). In the analysis of offspring (second generation), most traits that were positively correlated in mother generation now, also, showed a similar relationship. The only difference, in contrast to mother generation, was that, for most traits, the intensity of interaction increased

Natural mycobiota and aflatoxin B1 presence in bee pollen collected in Serbia

Total fungal count, incidence of fungi and aflatoxin B1 (AFB1) concentration were studied in 33 samples of bee pollen randomly collected from beekeepers in Serbia. The total number of fungi was determined by dilution method whereas AFB1 was detected using the Enzyme-Linked Immuno-Sorbent Assay (ELISA). The mycological estimation showed the presence of nine genera of fungi as followed: Acremonium, Alternaría, Aspergillus, Cladosporium, Epiccocum, Fusarium, Mucor, Pénicillium and Rhizopus, with total number ranging from 1 x 103 to 1 x 105 CFU g-1. The results have shown the predominance of the fungi from the genera Aspergillus and Alternaria. Among Aspergillus species it was observed that the most frequent species was A. flavus with incidence of 27.27 %. Mycotoxin AFB1 was detected as 100% positive in all samples (100%) with an average concentration of 8.61 μg kg-1. The obtained results indicated that honey bee pollen must be strictly controlled during its manipulation in the harvesting and manufacturing. Therefore, the implementation of good manufacturing (beekeeping) practice to define procedures for honeybee products could be crucial to reduce the risk of possible contamination and provide natural and safety product without risk on the human health

From error bounds to the complexity of first-order descent methods for convex functions

This paper shows that error bounds can be used as effective tools for deriving complexity results for first-order descent methods in convex minimization. In a first stage, this objective led us to revisit the interplay between error bounds and the Kurdyka-\L ojasiewicz (KL) inequality. One can show the equivalence between the two concepts for convex functions having a moderately flat profile near the set of minimizers (as those of functions with H\"olderian growth). A counterexample shows that the equivalence is no longer true for extremely flat functions. This fact reveals the relevance of an approach based on KL inequality. In a second stage, we show how KL inequalities can in turn be employed to compute new complexity bounds for a wealth of descent methods for convex problems. Our approach is completely original and makes use of a one-dimensional worst-case proximal sequence in the spirit of the famous majorant method of Kantorovich. Our result applies to a very simple abstract scheme that covers a wide class of descent methods. As a byproduct of our study, we also provide new results for the globalization of KL inequalities in the convex framework. Our main results inaugurate a simple methodology: derive an error bound, compute the desingularizing function whenever possible, identify essential constants in the descent method and finally compute the complexity using the one-dimensional worst case proximal sequence. Our method is illustrated through projection methods for feasibility problems, and through the famous iterative shrinkage thresholding algorithm (ISTA), for which we show that the complexity bound is of the form $O(q^{k})$ where the constituents of the bound only depend on error bound constants obtained for an arbitrary least squares objective with $\ell^1$ regularization