PREPARATION OF FUTURE TEACHERS FOR THE DEVELOPMENT OF GIFTEDNESS OF PRESCHOOL AND PRIMARY SCHOOL CHILDREN BY MEANS OF DRAMATIZATION

The necessity and main directions of preparation of future teachers for work with gifted children of preschool and primary school age are shown. The author's definition and structure of readiness of future educators to work with gifted children are presented. The signs of giftedness are analyzed and the characteristics of different types of giftedness are presented. The importance of identifying talented individuals in an educational institution and creating a developmental environment for the development of their abilities and creative self-realization is substantiated. The importance of theatrical activity for the development of general and special abilities, creativity, talent and the formation of non-cognitive qualities of preschool and primary school children is shown. The results of diagnostics of awareness of future teachers with the theoretical and methodological principles of using theatrical activities as a means of developing children's talents are presented. The main directions of training future teachers in the educational process of higher education institutions, aimed at mastering students' special knowledge, development of professional qualities, skills and abilities necessary for the use of dramatization in working with different categories of potentially gifted children

Meat productivity and quality of goose meat when using nanostructural zeolite in feeding

The article presents comparative studies of the meat productivity of geese of the Lindovskaya breed when zeolite and nanostructured zeolite feed additives are introduced into their diets. It was shown that the use of zeolite contributed to an increase in the live weight of geese by 4.0 %, the use of different doses of nanostructured zeolite led to an increase by 6.2–10.2 % (P≤0.05), in comparison with control analogues. When using zeolite in meat, the content of zinc and copper salts decreased by 28.0 and 13.1 %. The introduction of nanostructured zeolite into the diet led to a decrease in zinc content by 27.5–31.3 % and that of copper by 27.1–48.6 % compared with the control. The meat of birds of all groups corresponded to hygienic quality requirements for the content of chemical elements

Sanitary assessment and technological indicators of goat milk

Milk and dairy products are indispensable for the full development and functioning of the body. In recent years, the demand for goat’s milk has increased. It has long been considered a source of health, especially in relation to people suffering from allergic diseases (including cow milk protein), with metabolic disorders, digestive disorders, etc. That is why the problem of obtaining high-quality goat milk is relevant and practically significant. Over the past few decades, interest has grown in a variety of biologically active substances of natural origin for their use as components of the diet of farm animals. Unlike antibiotics, growth stimulants and other synthetic drugs, phytogenics can be used in healthy animals for most of the productive period, providing a positive result in terms of quantity and quality of products. Based on this, we studied for the first time the effect of the plant product of stevia processing on the veterinary and sanitary indicators of goat milk. An experimental group of goats received stevia pulp together with the main diet. During the experiment, we found positive dynamics associated with the productivity of the goats of the experimental group. The use of an experimental feed composition provided positive dynamics in terms of the main indicators characterizing the technological value of milk. Thus, based on the data obtained, it should be concluded that the use of stevia pulp in combination with the basic diet provides positive dynamics for technologically significant indicators of goat milk, as well as increases the productivity of animals and ensures the production of products with high veterinary and sanitary characteristics

Protocol for Increasing the Sensitivity of MS-Based Protein Detection in Human Chorionic Villi

An important step in the proteomic analysis of missing proteins is the use of a wide range of tissues, optimal extraction, and the processing of protein material in order to ensure the highest sensitivity in downstream protein detection. This work describes a purification protocol for identifying low-abundance proteins in human chorionic villi using the proposed “1DE-gel concentration” method. This involves the removal of SDS in a short electrophoresis run in a stacking gel without protein separation. Following the in-gel digestion of the obtained holistic single protein band, we used the peptide mixture for further LC–MS/MS analysis. Statistically significant results were derived from six datasets, containing three treatments, each from two tissue sources (elective or missed abortions). The 1DE-gel concentration increased the coverage of the chorionic villus proteome. Our approach allowed the identification of 15 low-abundance proteins, of which some had not been previously detected via the mass spectrometry of trophoblasts. In the post hoc data analysis, we found a dubious or uncertain protein (PSG7) encoded on human chromosome 19 according to neXtProt. A proteomic sample preparation workflow with the 1DE-gel concentration can be used as a prospective tool for uncovering the low-abundance part of the human proteome

Ni-loaded nanocrystalline ceria-zirconia solid solutions prepared via modified Pechini route as stable to coking catalysts of CH4 dry reforming

Mixed nanocrystalline Ce-Zr-O oxides (Ce/Zr = 1 or 7/3) were prepared by modified Pechini route using ethylene glycol solutions of metal salts. Detailed characterization of their real structure and surface properties by X-ray diffraction on synchrotron radiation with the full-profile Rietveld analysis, high resolution electron microscopy with elemental analysis, Raman spectroscopy, UV-Vis and X-ray photoelectron spectroscopy revealed a high homogeneity of cations distribution in nanodomains resulting in stabilization of disordered cubic phase. This provides a high dispersion of NiO loaded on these mixed oxides by wet impregnation, a high reactivity and mobility of oxygen in these catalysts and strong interaction of Ni with support in the reduced state. This helps to achieve a high activity and coking stability of developed catalysts in CH4 dry reforming in feeds with CH4 concentration up to 15% and CH4/CO2 ratio =1

Eradication of Therapy-resistant Human Prostate Tumors Using an Ultrasound-guided Site-specific Cancer Terminator Virus Delivery Approach

Intratumoral injections of a replication-incompetent adenovirus (Ad) expressing melanoma differentiation–associated gene-7/interleukin-24 (Ad.mda-7), a secreted cytokine displaying cancer-selective, apoptosis-inducing properties, profoundly inhibits prostate cancer (PC) growth in immune-incompetent animals. In contrast, Ad.mda-7 is ineffective in PCs overexpressing antiapoptotic proteins such as Bcl-2 or Bcl-xL. However, intratumoral injections of a conditionally replication-competent Ad (CRCA) in which expression of the adenoviral E1A gene is driven by the cancer-specific promoter of progression-elevated gene-3 (PEG-3) and which simultaneously expresses mda-7/interleukin (IL)-24 in the E3 region of the Ad (Ad.PEG-E1A-mda-7), a cancer terminator virus (CTV), is highly active in these cells. A major challenge for gene therapy is systemic delivery of nucleic acids directly into an affected tissue. Ultrasound (US) contrast agents (microbubbles—MBs) are viable candidates for gene delivery/therapy. Here, we show that MB/Ad.mda-7 complexes targeted to DU-145 cells using US dramatically reduced tumor burden in xenografted nude mice. Additionally, US-guided MB/CTV delivery completely eradicated not only targeted DU-145/Bcl-xL-therapy-resistant tumors, but also nontargeted distant tumors (established in the opposite flank), thereby implementing a cure. These findings highlight potential therapeutic applications of this novel image-guided gene therapy technology for advanced PC patients with metastatic disease