Life Tables of Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae): with a Mathematical Invalidation for Applying the Jackknife Technique to the Net Reproductive Rate

Life table data for the melon fly, Bactrocera cucurbitae (Coquillett), reared on cucumber (Cucumis sativus L.) were collected under laboratory and simulated field conditions. Means and standard errors of life table parameters were estimated for two replicates using the jackknife technique. At 25ºC, the intrinsic rates of increase (_r_) found for the two replicates were 0.1354 and 0.1002 day-1, and the net reproductive rates (_R_~0~) were 206.3 and 66.0 offspring, respectively. When the cucumbers kept under simulated field conditions were covered with leaves, the _r_ and _R_~0~ for the two replicates were 0.0935 and 0.0909 day-1, 17.5 and 11.4 offspring, respectively. However, when similar cucumbers were left uncovered, the _r_ and _R_~0~ for the two replicates were 0.1043 and 0.0904 day-1, and 27.7 and 10.1 offspring, respectively. Our results revealed that considerable variability between replicates in both laboratory and field conditions is possible; this variability should be taken into consideration in data collection and application of life tables. Mathematical analysis has demonstrated that applying the jackknife technique results in unrealistic pseudo-_R_~0~ and overestimation of its variance. We suggest that the jackknife technique should not be used for the estimation of variability of _R_~0~

Transport through a quantum ring, a dot and a barrier embedded in a nanowire in magnetic field

We investigate the transport through a quantum ring, a dot and a barrier embedded in a nanowire in a homogeneous perpendicular magnetic field. To be able to treat scattering potentials of finite extent in magnetic field we use a mixed momentum-coordinate representation to obtain an integral equation for the multiband scattering matrix. For a large embedded quantum ring we are able to obtain Aharanov-Bohm type of oscillations with superimposed narrow resonances caused by interaction with quasi-bound states in the ring. We also employ scattering matrix approach to calculate the conductance through a semi-extended barrier or well in the wire. The numerical implementations we resort to in order to describe the cases of weak and intermediate magnetic field allow us to produce high resolution maps of the ``near field'' scattering wave functions, which are used to shed light on the underlying scattering processes.Comment: RevTeX, 13 pages with included postscript figures, high resolution version available at http://hartree.raunvis.hi.is/~vidar/Rann/VG_04.pd

Designing Visible Light-Cured Thiol-Acrylate Hydrogels for Studying the HIPPO Pathway Activation in Hepatocellular Carcinoma Cells

Various polymerization mechanisms have been developed to prepare peptide-immobilized poly(ethylene glycol) (PEG) hydrogels, a class of biomaterials suitable for studying cell biology in vitro. Here, a visible light mediated thiol-acrylate photopolymerization scheme is reported to synthesize dually degradable PEG-peptide hydrogels with controllable crosslinking and degradability. The influence of immobilized monothiol pendant peptide is systematically evaluated on the crosslinking of these hydrogels. Further, methods are proposed to modulate hydrogel crosslinking, including adjusting concentration of comonomer or altering the design of multifunctional peptide crosslinker. Due to the formation of thioether ester bonds, these hydrogels are hydrolytically degradable. If the dithiol peptide linkers used are susceptible to protease cleavage, these thiol-acrylate hydrogels can be designed to undergo partial proteolysis. The differences between linear and multiarm PEG-acrylate (i.e., PEGDA vs PEG4A) are also evaluated. Finally, the use of the mixed-mode thiol-acrylate PEG4A-peptide hydrogels is explored for in situ encapsulation of hepatocellular carcinoma cells (Huh7). The effects of matrix stiffness and integrin binding motif (e.g., RGDS) on Huh7 cell growth and HIPPO pathway activation are studied using PEG4A-peptide hydrogels. This visible light poly-merized thiol-acrylate hydrogel system represents an alternative to existing light-cured hydrogel platforms and shall be useful in many biomedical applications

Buoyant surface jets discharged into a strong crossflow

Analytical and experimental investigations were carried out for three-dimensional buoyant surface jets with strong ambient flow but without boundary attachment. A numerical model in curvilinear coordinates was developed from an integral jet analysis modified for buoyancy effects and included asymmetry of the jet. Detailed temperature distributions were measured in the laboratory with densimetric Froude numbers (F̥) of 5, 10 and 15 and 0 velocity ratios (R) from 2 to 13. Experimental results showed that ambient crossflows can cause significant distortion of the jet, even for R = 13. Near the exit, the lower portion of the jet is swept toward the lee side of the jet. The resulting L-shaped cross section and the associated density instability may enhance spreading on the lee side and may contribute to the subsequent formation of bimodal temperature distributions. The jet bending increases as R decreases and as F̥ increases. Dilution increases with increasing F̥ and decreasing R. The model was calibrated against the entire set of measured temperatures for each run and is capable of predicting temperature 0 distributions to an accuracy of 0.63C˚. The agreement could probably be improved by using similarity profiles better suited to the actual jet cross sectional shape which was not known at the beginning of the research.U.S. Department of the InteriorU.S. Geological SurveyOpe

First-principles method of propagation of tightly bound excitons: exciton band structure of LiF and verification with inelastic x-ray scattering

We propose a simple first-principles method to describe propagation of tightly bound excitons. By viewing the exciton as a composite object (an effective Frenkel exciton in Wannier orbitals), we define an exciton kinetic kernel to encapsulate the exciton propagation and decay for all binding energy. Applied to prototypical LiF, our approach produces three exciton bands, which we verified quantitatively via inelastic x-ray scattering. The proposed real-space picture is computationally inexpensive and thus enables study of the full exciton dynamics, even in the presence of surfaces and impurity scattering. It also provides intuitive understanding to facilitate practical exciton engineering in semiconductors, strongly correlated oxides, and their nanostructures.Comment: 5 pages, 4 figures. Accepted by PR

Chemoenzymatic elaboration of monosaccharides using engineered cytochrome P450_(BM3) demethylases

Polysaccharides comprise an extremely important class of biopolymers that play critical roles in a wide range of biological processes, but the synthesis of these compounds is challenging because of their complex structures. We have developed a chemoenzymatic method for regioselective deprotection of monosaccharide substrates using engineered Bacillus megaterium cytochrome P450 (P450_(BM3)) demethylases that provides a highly efficient means to access valuable intermediates, which can be converted to a wide range of substituted monosaccharides and polysaccharides. Demethylases displaying high levels of regioselectivity toward a number of protected monosaccharides were identified using a combination of protein and substrate engineering, suggesting that this approach ultimately could be used in the synthesis of a wide range of substituted mono- and polysaccharides for studies in chemistry, biology, and medicine

Study of stratified overflows and underflows

The study presents a general analysis of two-layered stratified flows taking into account effects of sidewall friction and variation of density with horizontal distance. The analysis is applied to the study of arrested thermal wedges and arrested cold water intrusions. Laboratory data were collected and analyzed and it was determined that bed roughness has a significant effect on the interfacial friction factor for arrested thermal wedges but not for arrested cold water intrusions. Friction factors were found to vary significantly along the wedges indicating that use of average values is perhaps undesirable. Local values of friction factor were found to increase with values of a local interfacial Reynolds number.U.S. Department of the InteriorU.S. Geological SurveyOpe