Upregulation of Id1 by Epstein-Barr Virus-encoded LMP1 confers resistance to TGFβ-mediated growth inhibition

BACKGROUND: Epstein-Barr virus (EBV)-encoded LMP1 protein is commonly expressed in nasopharyngeal carcinoma (NPC). LMP1 is a prime candidate for driving tumourigenesis given its ability to activate multiple signalling pathways and to alter the expression and activity of variety of downstream targets. Resistance to TGFβ-mediated cytostasis is one of the growth transforming effects of LMP1. Of the downstream targets manipulated by LMP1, the induction of Id1 and inactivation of Foxo3a appear particularly relevant to LMP1-mediated effects. Id1, a HLH protein is implicated in cell transformation and plays a role in cell proliferation, whilst Foxo3a, a transcription factor controls cell integrity and homeostasis by regulating apoptosis. The mechanism(s) by which LMP1 induces these effects have not been fully characterised. RESULTS: In this study, we demonstrate that the ability of LMP1 to induce the phosphorylation and inactivation of Foxo3a is linked to the upregulation of Id1. Furthermore, we show that the induction of Id1 is essential for the transforming function of LMP1 as over-expression of Id1 increases cell proliferation, attenuates TGFβ-SMAD-mediated transcription and renders cells refractory to TGFβ-mediated cytostasis. Id1 silencing in LMP1-expressing epithelial cells abolishes the inhibitory effect of LMP1 on TGFβ-mediated cell growth arrest and reduces the ability of LMP1 to attenuate SMAD transcriptional activity. In response to TGFβ stimulation, LMP1 does not abolish SMAD phosphorylation but inhibits p21 protein expression. In addition, we found the induction of Id1 in LMP1-expressing cells upon stimulation by TGFβ. We provide evidence that LMP1 suppresses the transcriptional repressor ATF3, possibly leading to the TGFβ-induced Id1 upregulation. CONCLUSION: The current data provide novel information regarding the mechanisms by which LMP1 suppresses TGFβ-induced cytostasis, highlighting the importance of Id1 in LMP1 mediated cell transformatio

Quantum Gambling Using Three Nonorthogonal States

We provide a quantum gambling protocol using three (symmetric) nonorthogonal states. The bias of the proposed protocol is less than that of previous ones, making it more practical. We show that the proposed scheme is secure against non-entanglement attacks. The security of the proposed scheme against entanglement attacks is shown heuristically.Comment: no essential correction, 4 pages, RevTe

Heisenberg-picture approach to the exact quantum motion of a time-dependent forced harmonic oscillator

In the Heisenberg picture, the generalized invariant and exact quantum motions are found for a time-dependent forced harmonic oscillator. We find the eigenstate and the coherent state of the invariant and show that the dispersions of these quantum states do not depend on the external force. Our formalism is applied to several interesting cases.Comment: 15 pages, two eps files, to appear in Phys. Rev. A 53 (6) (1996

Glutamate Receptors GluR1 and GluR4 in the Hamster Superior Colliculus: Distribution and Co-localization with Calcium-Binding Proteins and GABA

We investigated the distributions of AMPA glutamate receptor subtypes GluR1 and GluR4 in the hamster superior colliculus (SC) with antibody immunocytochemistry and the effect of enucleation on these distributions. We compared these labelings to those of GluR2/3 in our previous report (Park et al., 2004, Neurosci Res., 49:139–155) and calcium-binding proteins calbindin D28K, calretinin, parvalbumin, and GABA. Anti-GluR1-immunoreactive (IR) cells were scattered throughout the SC. By contrast, anti-GluR4-IR cells formed distinct clusters within the lower lateral stratum griseum intermediale (SGI) and lateral stratum album intermediale (SAI). The GluR1- and GluR4-IR neurons varied in size and morphology. The average diameter of the GluR1-IR cells was 13.00 µm, while the GluR4-IR cells was 20.00 µm. The large majority of IR neurons were round or oval cells, but they also included stellate, vertical fusiform and horizontal cells. Monocular enucleation appeared to have no effect on the GluR1 and GluR4 immunoreactivity. Some GluR1-IR cells expressed calbindin D28K (9.50%), calretinin (6.59%), parvalbumin (2.53%), and GABA (20.54%). By contrast, no GluR4-IR cells expressed calcium-binding proteins or GABA. Although the function of the AMPA receptor subunits in SC is not yet clear, the distinct segregation of the GluR subunits, its differential colocalization with calcium-binding proteins and GABA, and differential responses to enucleation suggest the functional diversity of the receptor subunits in visuo-motor integration in the SC

Gas distribution, kinematics and star formation in faint dwarf galaxies

We compare the gas distribution, kinematics and the current star formation in a sample of 10 very faint (-13.37 < M_B < -9.55) dwarf galaxies. For 5 of these galaxies we present fresh, high sensitivity, GMRT HI 21cm observations. For all our galaxies we construct maps of the HI column density at a constant linear resolution of ~300 pc; this forms an excellent data set to check for the presence of a threshold column density for star formation. We find that while current star formation (as traced by Halpha emission) is confined to regions with relatively large (N_HI > (0.4 -1.7) X 10^{21} atoms cm^{-2}) HI column density, the morphology of the Halpha emission is in general not correlated with that of the high HI column density gas. Thus, while high column density gas may be necessary for star formation, in this sample at least, it is not sufficient to ensure that star formation does in fact occur. We examine the line profiles of the HI emission, but do not find a simple relation between regions with complex line profiles and those with on-going star formation. Finally, we examine the very fine scale (~20-100 pc) distribution of the HI gas, and find that at these scales the emission exhibits a variety of shell like, clumpy and filamentary features. The Halpha emission is sometimes associated with high density HI clumps, sometimes the Halpha emission lies inside a high density shell, and sometimes there is no correspondence between the Halpha emission and the HI clumps. In summary, the interplay between star formation and gas density in these galaxy does not seem to show the simple large scale patterns observed in brighter galaxies (abridged).Comment: 15 pages, 6 tables, 13 figures. Accepted for publication in MNRA

Shor-Preskill Type Security-Proofs for Concatenated Bennett-Brassard 1984 Quantum Key Distribution Protocol

We discuss long code problems in the Bennett-Brassard 1984 (BB84) quantum key distribution protocol and describe how they can be overcome by concatenation of the protocol. Observing that concatenated modified Lo-Chau protocol finally reduces to the concatenated BB84 protocol, we give the unconditional security of the concatenated BB84 protocol.Comment: 4 pages, RevTe

Quantum Gambling Using Two Nonorthogonal States

We give a (remote) quantum gambling scheme that makes use of the fact that quantum nonorthogonal states cannot be distinguished with certainty. In the proposed scheme, two participants Alice and Bob can be regarded as playing a game of making guesses on identities of quantum states that are in one of two given nonorthogonal states: if Bob makes a correct (an incorrect) guess on the identity of a quantum state that Alice has sent, he wins (loses). It is shown that the proposed scheme is secure against the nonentanglement attack. It can also be shown heuristically that the scheme is secure in the case of the entanglement attack.Comment: no essential correction, 4 pages, RevTe

Non-Abelian Chern-Simons Particles and their Quantization

A many--body Schr\"odinger equation for non--Abelian Chern--Simons particles is obtained from both point--particle and field--theoretic pictures. We present a particle Lagrangian and a field theoretic Lagrange density, and discuss their properties. Both are quantized by the symplectic method of Hamiltonian reduction. An $N$--body Schr\"odinger equation for the particles is obtained from both starting points. It is shown that the resulting interaction between particles can be replaced by non--trivial boundary conditions. Also, the equation is compared with the one given in the literature.Comment: 18 pages, MIT preprint CTP # 227

Galaxy Evolution and Star Formation Efficiency at 0.2 < z < 0.6

We present the results of a CO line survey of 30 galaxies at moderate redshift (z \sim 0.2-0.6), with the IRAM 30m telescope, with the goal to follow galaxy evolution and in particular the star formation efficiency (SFE) as defined by the ratio between far-infrared luminosity and molecular gas mass (LFIR/M(H2)). The sources are selected to be ultra-luminous infrared galaxies (ULIRGs), with LFIR larger than 2.8 10^{12} Lsol, experiencing starbursts; adopting a low ULIRG CO-to-H2 conversion factor, their gas consumption time-scale is lower than 10^8 yr. To date only very few CO observations exist in this redshift range that spans nearly 25% of the universe's age. Considerable evolution of the star formation rate is already observed during this period. 18 galaxies out of our sample of 30 are detected (of which 16 are new detections), corresponding to a detection rate of 60%. The average CO luminosity for the 18 galaxies detected is L'CO = 2 10^{10} K km/s pc^2, corresponding to an average H2 mass of 1.6 10^{10} Msol. The FIR luminosity correlates well with the CO luminosity, in agreement with the correlation found for low and high redshift ULIRGs. Although the conversion factor between CO luminosity and H2 mass is uncertain, we find that the maximum amount of gas available for a single galaxy is quickly increasing as a function of redshift. Using the same conversion factor, the SFEs for z\sim 0.2-0.6 ULIRGs are found to be significantly higher, by a factor 3, than for local ULIRGs, and are comparable to high redshift ones. We compare this evolution to the expected cosmic H2 abundance and the cosmic star formation history.Comment: 11 pages, 13 figures, accepted in A&