15 research outputs found
Vaccination with Human Induced Pluripotent Stem Cells Creates an Antigen-Specific Immune Response Against HIV-1 gp160
Induced pluripotent stem cells (iPSCs) are artificially derived from somatic cells that have been transduced with defined reprogramming factors. A previous report has indicated the possibility of using iPSCs as an immune stimulator to generate antigen-specific immunity. In our current study, we have investigated whether human iPSCs (hiPSCs) have the ability to enhance specific immune response against a human immunodeficiency virus type 1 (HIV-1) antigen in a xenogenic mouse model. Our results show that BALB/c mice immunized with hiPSCs transduced with an adenoviral vector encoding HIV-1 gp160 exhibited prominent antigen-specific cellular immune responses. We further found that pre-treatment of hiPSCs with ionizing radiation promotes the secretion of pro-inflammatory cytokines such as interleukin-1 alpha (IL-1α), IL-12, and IL-18. These cytokines might promote the activation of antigen-presenting cells and the effective induction of cellular immunity. Our present findings thus demonstrate that a hiPSCs-based vaccine has the potential to generate cellular immunity against viral antigens such as HIV-1 gp160 in a xenogenic condition
Dual-frequency injection-locked continuous-wave near-infrared laser
We report a dual-frequency injection-locked continuous-wave near-infrared
laser. The entire system consists of a Ti:sapphire ring laser as a power
oscillator, two independent diode-lasers employed as seed lasers, and a master
cavity providing a frequency reference. Stable dual-frequency injection-locked
oscillation is achieved with a maximum output power of 2.8 W. As fundamental
performance features of this laser system, we show its single
longitudinal/transverse mode characteristics and practical power stability.
Furthermore, as advanced features, we demonstrate arbitrary selectivity of the
two frequencies and flexible control of their relative powers by simply
manipulating the seed lasers.Comment: 8 pages, 4 figure
Changes in the Baiu Frontal Activity in the Future Climate Simulated by Super-High-Resolution Global and Cloud-Resolving Regional Climate Models
Importance of Determination of Crystal Quality in Protein Crystals when Performing High-Resolution Structural Analysis
Trans-Bilayer Ion Conduction by Proline Containing Cyclic Hexapeptides and Effects of Amino Acid Substitutions on Ion Conducting Properties
Cardiac Magnetic Resonance Imaging of Very Late Intrapericardial Hematoma 8 Years after Coronary Artery Bypass Grafting
DHA meta-analysis data
Percentage of DHA in total fatty acid were obtained from literatures. DHA contents in algae and consumers from marine and freshwater habitats were analyzed
A key metabolic gene for recurrent freshwater colonization and radiation in fishes
Colonization of new ecological niches has triggered large adaptive radiations. Although some lineages have made use of such opportunities, not all do so. The factors causing this variation among lineages are largely unknown. Here, we show that deficiency in docosahexaenoic acid (DHA), an essential ω-3 fatty acid, can constrain freshwater colonization by marine fishes. Our genomic analyses revealed multiple independent duplications of the fatty acid desaturase gene; Fads2; in stickleback lineages that subsequently colonized and radiated in freshwater habitats, but not in close relatives that failed to colonize. Transgenic manipulation of; Fads2; in marine stickleback increased their ability to synthesize DHA and survive on DHA-deficient diets. Multiple freshwater ray-finned fishes also show a convergent increase in; Fads2; copies, indicating its key role in freshwater colonization
Data from: A key metabolic gene for recurrent freshwater colonization and radiation in fishes
Colonization of new ecological niches has triggered large adaptive radiations. Although some lineages have made use of such opportunities, not all do so. The factors causing this variation among lineages are largely unknown. Here, we show that deficiency in docosahexaenoic acid (DHA), an essential ω-3 fatty acid, can constrain freshwater colonization by marine fishes. Our genomic analyses revealed multiple independent duplications of the fatty acid desaturase gene Fads2 in stickleback lineages that subsequently colonized and radiated in freshwater habitats, but not in close relatives that failed to colonize. Transgenic manipulation of Fads2 in marine stickleback increased their ability to synthesize DHA and survive on DHA-deficient diets. Multiple freshwater ray-finned fishes also show a convergent increase in Fads2 copies, indicating its key role in freshwater colonization