The potential of natural, photosynthetic pigments to improve the efficiency of dye-sensitized solar cells

Gemstone Team GrenergyCurrent photovoltaic cells incorporate silicon or synthetic dyes; however, these cells are expensive and the dyes are toxic. Our product uses natural, photosynthetic pigments to sensitize an alternative design solar cell, the dye-sensitized solar cell (DSSC). Research has shown that plant pigments are suitable sensitizers for these cells, but there is presently no good rationale to determine which pigment combinations may be most effective. Our research goal was to develop and test an absorption index for pigment selection that would increase the output of DSSCs. Our results demonstrated a positive correlation between spectral absorption of the sensitizing dye and power output of the cell. Certain pigment combinations were more effective sensitizers based on combined absorption capabilities, but resolving the mechanisms of the exact relationship requires further research and likely further development of the algorithm used to choose optimal pigment combinations

Host cell polarity proteins participate in innate immunity to Pseudomonas aeruginosa infection

The mucosal epithelium consists of polarized cells with distinct apical and basolateral membranes that serve as functional and physical barriers to external pathogens. The apical surface of the epithelium constitutes the first point of contact between mucosal pathogens, such as Pseudomonas aeruginosa, and their host. We observed that binding of P. aeruginosa aggregates to the apical surface of polarized cells led to the striking formation of an actin-rich membrane protrusion with inverted polarity, containing basolateral lipids and membrane components. Such protrusions were associated with a spatially localized host immune response to P. aeruginosa aggregates that required bacterial flagella and a type III secretion system apparatus. Host protrusions formed de novo underneath bacterial aggregates and involved the apical recruitment of a Par3/Par6α/aPKC/Rac1 signaling module for a robust, spatially localized host NF-κB response. Our data reveal a role for spatiotemporal epithelial polarity changes in the activation of innate immune responses

A streptococcal protease that degrades CXC chemokines and impairs bacterial clearance from infected tissues

Group A Streptococcus (GAS) causes the life-threatening infection in humans known as necrotizing fasciitis (NF). Infected subcutaneous tissues from an NF patient and mice challenged with the same GAS strain possessed high bacterial loads but a striking paucity of infiltrating polymorphonuclear leukocytes (PMNs). Impaired PMN recruitment was attributed to degradation of the chemokine IL-8 by a GAS serine peptidase. Here, we use bioinformatics approach coupled with target mutagenesis to identify this peptidase as ScpC. We show that SilCR pheromone downregulates scpC transcription via the two-component system—SilA/B. In addition, we demonstrate that in vitro, ScpC degrades the CXC chemokines: IL-8 (human), KC, and MIP-2 (both murine). Furthermore, using a murine model of human NF, we demonstrate that ScpC, but not the C5a peptidase ScpA, is an essential virulence factor. An ScpC-deficient mutant is innocuous for untreated mice but lethal for PMN-depleted mice. ScpC degrades KC and MIP-2 locally in the infected skin tissues, inhibiting PMN recruitment. In conclusion, ScpC represents a novel GAS virulence factor functioning to directly inactivate a key element of the host innate immune response