Comparison of Passive Microwave Data with Shipborne Photographic Observations of Summer Sea Ice Concentration along an Arctic Cruise Path

Arctic sea ice concentration (SIC) has been studied extensively using passive microwave (PM) remote sensing. This technology could be used to improve navigation along vessel cruise paths; however, investigations on this topic have been limited. In this study, shipborne photographic observation (P-OBS) of sea ice was conducted using oblique-oriented cameras during the Chinese National Arctic Research Expedition in the summer of 2016. SIC and the areal fractions of open water, melt ponds, and sea ice (Aw, Ap, and Ai, respectively) were determined along the cruise path. The distribution of SIC along the cruise path was U-shaped, and open water accounted for a large proportion of the path. The SIC derived from the commonly used PM algorithms was compared with the moving average (MA) P-OBS SIC, including Bootstrap and NASA Team (NT) algorithms based on Special Sensor Microwave Imager/Sounder (SSMIS) data; and ARTIST sea ice, Bootstrap, Sea Ice Climate Change Initiative, and NASA Team 2 (NT2) algorithms based on Advanced Microwave Scanning Radiometer 2 (AMSR2) data. P-OBS performed better than PM remote sensing at detecting low SIC (< 10%). Our results indicate that PM SIC overestimates MA P-OBS SIC at low SIC, but underestimates it when SIC exceeds a turnover point (TP). The presence of melt ponds affected the accuracy of the PM SIC; the PM SIC shifted from an overestimate to an underestimate with increasing Ap, compared with MA P-OBS SIC below the TP, while the underestimation increased above the TP. The PM algorithms were then ranked; SSMIS-NT and AMSR2-NT2 are the best and worst choices for Arctic navigation, respectively

Laboratory experimental study of water drag force exerted on ridge keel

With the diminishing Arctic sea ice, the dynamic energy-exchange process between sea ice and ocean gains in importance. Concerning how the ice bottom topography affects the drift of sea ice, it is unclear how the ridge–keel-drag force exerted by seawater changes the momentum balance of sea ice. We thus conducted laboratory experiments to investigate how the local drag coefficient of the ridge keel depends on keel shape and on the relative velocity of ice with respect to seawater. A dimensional analysis is used to obtain the relationship between the local drag coefficient Cr, the Reynolds number Re, the dimensionless keel depth h0, and the keel slope angle φ. The results indicate that the local drag coefficient Cr is only relevant to Re when Re < 4000 and the flow is in the laminar regime. With increasing Re, Cr depends on h0 and φ, which are independent variables, as the flow transitions to the turbulent regime. The parameterization formulas for Cr are also provided

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field