Experimental Study on Pervious Concrete: An Eco Friendly Concrete Pavement

As a civil engineer and human being it's our prime duty prime to save environment, because lack of water absorption and air permeability of common concrete pavement, rain water is not entered in to the ground directly. It will reduce ground water table, plants are difficult to grow normally, difficult to maintain temperature and humidity of earth. To minimize such affects, the research on pervious concrete pavement widely done for road way application. In this study, determine compressive strength, porosity test on pervious concrete. The compressive strength is relatively low because of its porosity but at the same time we increase water absorption quality. Due to low strength we cannot be used as a road pavement. The pervious concrete can only be applied to footpaths, parking and where low strength is required

EXPLORING TAXPAYER AWARENESS, PERCEPTIONS, AND SATISFACTION: AN IN-DEPTH STUDY OF ELECTRONIC INCOME TAX FILING IN DHULE AND NANDURBAR DISTRICTS

This research paper delves into the realm of taxpayer perceptions and actions concerning the electronic filing of income tax returns, focusing on the Dhule and Nandurbar districts. The primary objective of this study is to comprehensively investigate the prevailing attitudes towards electronic filing, while also evaluating the level of taxpayer engagement in these districts. The research is underpinned by three key dimensions: general attitudes, understanding, and satisfaction with the electronic filing system. The study employs a mixed-methods approach, incorporating surveys and interviews to gather insights from a diverse pool of taxpayers. Firstly, the research aims to unravel the general attitudes that taxpayers hold toward the electronic filing system. Secondly, it seeks to gauge the depth of taxpayers' comprehension regarding the process of electronically filing income tax returns. Lastly, the study aspires to measure the level of satisfaction that respondents derive from the electronic filing system. With a focus on Dhule and Nandurbar districts, the findings of this research provide valuable insights into the effectiveness of electronic filing implementation and its reception among taxpayers. This study not only contributes to the existing literature on tax compliance behavior but also offers practical implications for policymakers and tax authorities aiming to enhance the efficiency and user-friendliness of electronic tax filing systems

Implementation of the Ethereum Blockchain for Supply Chain Management

This research looks at unique identity systems in supply chain management and how self-sovereign identities foster trust among the issuer, entity, and verifier. The article investigates blockchain technology in supply chain systems. According to recent research, blockchain applications may increase transparency, traceability, and efficiency in manufacturing and distribution. To increase privacy and transparency in end-to-end monitoring, the research advises incorporating self-sovereign identity into the supply chain. The study examines blockchain performance, including transaction and read throughput, latency, and resource utilization, using a hyperledger caliper to analyze supply chain performance. The research finishes with supply chain management issues. It is challenging to manage product and process integrity in a multi-stakeholder supply chain. Many current solutions suffer from data fragmentation, uneven provenance, and differing protocol limitations between distributions and processes. Blockchain is a pioneering technology that allows secure traceability and control, immutability, and stakeholder trust at a low cost. Blockchain use is increasing in many sectors, but supply chains confront substantial challenges in implementing it. This is the first report to look at blockchain-enabled supply chain concerns and prospects. We look at supply chain digitalization as well as GS1 standards and technologies. Compile a list of blockchain-enabled supply chain use cases and businesses. The technical and non-technical impediments to blockchain adoption for supply chain applications are investigated, as well as the applicability of consensus algorithms. Diagrams and assessments of blockchain ecosystem technology and tools Some critical future research areas are also suggested in order to achieve widespread blockchain-based supply chain traceability adoption. Finally, we introduce MOHBS Chain, a novel supply chain architecture powered by blockchai

Virtual screening of synthesized thiazole derivatives for M. tuberculosis and dTDP-rhamnose inhibitors

To determine antimycobacterium and dTDP rhamnose inhibitor activity of the synthesized azetidinone, thiazolidinone derivatives of thiazole, we studied different derivatives for the activity. One pot synthesis of 2-amino-4-methylthiazole-5-carboxylic acid ethyl ester has been carried out and synthesized different derivative compounds. Compounds were tested for antimicrobial activity against different strains of microorganism and antitubercular activity against M. tuberculosis H37Rv. Compounds 7c, 7d, 7i, 8d, 8e, 8g and 8h, were showed antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhosa using Gentamycin as standard, while 7b, 7e, 7f, 7i, 8b, 8e, 8f and 8i showed very strong antimycobacterial activity using rifampicine as a standard. Thiazole derivatives especially with carbonyl group scaffold inhibit an enzyme RmlC, which is an essential component for the biosynthesis of dTDP-rhamnose and produce good antimycobacterium and antimicrobial activity. Keywords: Thiazole, thiazolidinone derivatives, azetidinone derivative, well diffusion method, broth microdilution assay, antitubercular activity, antimicrobial activity

Discovery and characterization of small molecule Rac1 inhibitors

Aberrant activation of Rho GTPase Rac1 has been observed in various tumor types, including pancreatic cancer. Rac1 activates multiple signaling pathways that lead to uncontrolled proliferation, invasion and metastasis. Thus, inhibition of Rac1 activity is a viable therapeutic strategy for proliferative disorders such as cancer. Here we identified small molecule inhibitors that target the nucleotide-binding site of Rac1 through in silico screening. Follow up in vitro studies demonstrated that two compounds blocked active Rac1 from binding to its effector PAK1. Fluorescence polarization studies indicate that these compounds target the nucleotide-binding site of Rac1. In cells, both compounds blocked Rac1 binding to its effector PAK1 following EGF-induced Rac1 activation in a dose-dependent manner, while showing no inhibition of the closely related Cdc42 and RhoA activity. Furthermore, functional studies indicate that both compounds reduced cell proliferation and migration in a dose-dependent manner in multiple pancreatic cancer cell lines. Additionally, the two compounds suppressed the clonogenic survival of pancreatic cancer cells, while they had no effect on the survival of normal pancreatic ductal cells. These compounds do not share the core structure of the known Rac1 inhibitors and could serve as additional lead compounds to target pancreatic cancers with high Rac1 activity

A comprehensive review of portable cold storage: Technologies, applications, and future trends

In recent years, there has been a substantial increase in the usage of portable cold storage technologies, as the demand for flexible and mobile solutions for storing perishable goods has expanded. The advantages of portable cold storage units include energy efficiency, portability, and use. This analysis examines portable cold storage technologies, their uses, and future prospects. We also examine the use of phase change materials (PCMs) in conjunction with portable cold storage units for the storage of perishable food items such as fruits and vegetables. Beginning with an introduction to the various types of portable cold storage units, including refrigerated shipping containers, portable refrigerators, and cold rooms, the study continues with a discussion of the advantages and disadvantages of each. The various methods employed in portable cold storage units, such as compression refrigeration, absorption refrigeration, and thermoelectric cooling, are then discussed. The analysis also addresses the uses of portable cold storage units, including those in the food & beverage, pharmaceutical, and medical industries. The usage of PCMs in conjunction with portable cold storage units is then described, along with the various types and qualities of PCMs. The report continues with a consideration of future prospects in portable cold storage technologies, such as using renewable energy sources, intelligent sensors, and the Internet of Things

Characterization of CDK(5) Inhibitor, 20-223 (aka CP668863) for Colorectal Cancer Therapy

Colorectal cancer (CRC) remains one of the leading causes of cancer related deaths in the United States. Currently, there are limited therapeutic options for patients suffering from CRC, none of which focus on the cell signaling mechanisms controlled by the popular kinase family, cyclin dependent kinases (CDKs). Here we evaluate a Pfizer developed compound, CP668863, that inhibits cyclin-dependent kinase 5 (CDK5) in neurodegenerative disorders. CDK5 has been implicated in a number of cancers, most recently as an oncogene in colorectal cancers. Our lab synthesized and characterized CP668863 – now called 20-223. In our established colorectal cancer xenograft model, 20-223 reduced tumor growth and tumor weight indicating its value as a potential anti-CRC agent. We subjected 20-223 to a series of cell-free and cell-based studies to understand the mechanism of its anti-tumor effects. In our hands, in vitro 20-223 is most potent against CDK2 and CDK5. The clinically used CDK inhibitor AT7519 and 20-223 share the aminopyrazole core and we used it to benchmark the 20-223 potency. In CDK5 and CDK2 kinase assays, 20-223 was ~3.5-fold and ~65.3-fold more potent than known clinically used CDK inhibitor, AT7519, respectively. Cell-based studies examining phosphorylation of downstream substrates revealed 20-223 inhibits the kinase activity of CDK5 and CDK2 in multiple CRC cell lines. Consistent with CDK5 inhibition, 20-223 inhibited migration of CRC cells in a wound-healing assay. Profiling a panel of CRC cell lines for growth inhibitory effects showed that 20-223 has nanomolar potency across multiple CRC cell lines and was on an average \u3e2-fold more potent than AT7519. Cell cycle analyses in CRC cells revealed that 20-223 phenocopied the effects associated with AT7519. Collectively, these findings suggest that 20-223 exerts anti-tumor effects against CRC by targeting CDK 2/5 and inducing cell cycle arrest. Our studies also indicate that 20-223 is a suitable lead compound for colorectal cancer therapy

Genetic impairment of succinate metabolism disrupts bioenergetic sensing in adrenal neuroendocrine cancer

Metabolic dysfunction mutations can impair energy sensing and cause cancer. Loss of function of the mitochondrial tricarboxylic acid (TCA) cycle enzyme subunit succinate dehydrogenase B (SDHB) results in various forms of cancer typified by pheochromocytoma (PC). Here we delineate a signaling cascade where the loss of SDHB induces the Warburg effect, triggers dysregulation of [Ca2+]i, and aberrantly activates calpain and protein kinase Cdk5, through conversion of its cofactor from p35 to p25. Consequently, aberrant Cdk5 initiates a phospho-signaling cascade where GSK3 inhibition inactivates energy sensing by AMP kinase through dephosphorylation of the AMP kinase γ subunit, PRKAG2. Overexpression of p25-GFP in mouse adrenal chromaffin cells also elicits this phosphorylation signaling and causes PC. A potent Cdk5 inhibitor, MRT3-007, reverses this phospho-cascade, invoking a senescence-like phenotype. This therapeutic approach halted tumor progression in vivo. Thus, we reveal an important mechanistic feature of metabolic sensing and demonstrate that its dysregulation underlies tumor progression in PC and likely other cancers

Hybrid hydrodynamic cavitation (HC) technique for the treatment and disinfection of lake water

Water reclamation from lakes needs to be accomplished efficiently and affordably to ensure the availability of clean, disinfected water for society. Previous treatment techniques, such as coagulation, adsorption, photolysis, ultraviolet light, and ozonation, are not economically feasible on a large scale. This study investigated the effectiveness of standalone HC and hybrid HC + H2O2 treatment techniques for treating lake water. The effect of pH (3 to 9), inlet pressure (4 to 6 bar), and H2O2 loading (1 to 5 g/L) were examined. At pH = 3, inlet pressure of 5 bar and H2O2 loadings of 3 g/L, maximum COD and BOD removal were achieved·H2O2 was observed to significantly improve the performance of the HC when used as a chemical oxidant. In an optimal operating condition, a COD removal of 54.5 % and a BOD removal of 51.5 % using HC alone for 1 h is observed. HC combined with H2O2 removed 64 % of both COD and BOD. The hybrid HC + H2O2 treatment technique resulted in a nearly 100% removal of pathogens. The results of this study indicate that the HC-based technique is an effective method for removing contaminants and disinfection of the lake water