L-tryptophan photoproduct : effect on DNA replication and DNA repair in escherichia coli

Includes vita."This study was stimulated by two important discoveries. The first of these came to a focus when it was observed that recombinationless (rec) mutants of bacteria were readily killed by near-UV irradiation. Recognition of the deleterious effects of near-UV has a long history (see REVIEW OF LITERATURE), but the availability of the particularly sensitive rec mutants allowed one to ask direct questions about the chromophore (the first molecule altered by the near-UV energy), and the target (the cellular entity altered to produce the observed biological effect), involved in this radiation sensitivity. The second discovery was that the amino acid tryptophan, when irradiated with near-UV energy, yields a photoproduct that is lethal for rec mutants. Thus, a specific chromophore was identified. These discoveries set the stage for studies on the effect of the chromophore on the target. After many exploratory trials, three areas were chosen for experiments: (1) the effect of the tryptophan photoproduct (TP) on DNA replication and cell division, (2) the effect of TP on DNA repair, and (3) interaction of TP and monochromatic 365 nm radiation on bacteria."--Introduction.Includes bibliographical references

Large-amplitude driving of a superconducting artificial atom: Interferometry, cooling, and amplitude spectroscopy

Superconducting persistent-current qubits are quantum-coherent artificial atoms with multiple, tunable energy levels. In the presence of large-amplitude harmonic excitation, the qubit state can be driven through one or more of the constituent energy-level avoided crossings. The resulting Landau-Zener-Stueckelberg (LZS) transitions mediate a rich array of quantum-coherent phenomena. We review here three experimental works based on LZS transitions: Mach-Zehnder-type interferometry between repeated LZS transitions, microwave-induced cooling, and amplitude spectroscopy. These experiments exhibit a remarkable agreement with theory, and are extensible to other solid-state and atomic qubit modalities. We anticipate they will find application to qubit state-preparation and control methods for quantum information science and technology.Comment: 13 pages, 5 figure

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

The role of networks to overcome large-scale challenges in tomography : the non-clinical tomography users research network

Our ability to visualize and quantify the internal structures of objects via computed tomography (CT) has fundamentally transformed science. As tomographic tools have become more broadly accessible, researchers across diverse disciplines have embraced the ability to investigate the 3D structure-function relationships of an enormous array of items. Whether studying organismal biology, animal models for human health, iterative manufacturing techniques, experimental medical devices, engineering structures, geological and planetary samples, prehistoric artifacts, or fossilized organisms, computed tomography has led to extensive methodological and basic sciences advances and is now a core element in science, technology, engineering, and mathematics (STEM) research and outreach toolkits. Tomorrow's scientific progress is built upon today's innovations. In our data-rich world, this requires access not only to publications but also to supporting data. Reliance on proprietary technologies, combined with the varied objectives of diverse research groups, has resulted in a fragmented tomography-imaging landscape, one that is functional at the individual lab level yet lacks the standardization needed to support efficient and equitable exchange and reuse of data. Developing standards and pipelines for the creation of new and future data, which can also be applied to existing datasets is a challenge that becomes increasingly difficult as the amount and diversity of legacy data grows. Global networks of CT users have proved an effective approach to addressing this kind of multifaceted challenge across a range of fields. Here we describe ongoing efforts to address barriers to recently proposed FAIR (Findability, Accessibility, Interoperability, Reuse) and open science principles by assembling interested parties from research and education communities, industry, publishers, and data repositories to approach these issues jointly in a focused, efficient, and practical way. By outlining the benefits of networks, generally, and drawing on examples from efforts by the Non-Clinical Tomography Users Research Network (NoCTURN), specifically, we illustrate how standardization of data and metadata for reuse can foster interdisciplinary collaborations and create new opportunities for future-looking, large-scale data initiatives

Phototoxicity of near-UV irradiated L-tryptophan for recombinationless mutants of Salmonella typhimurium

Digitized by Kansas Correctional Industrie

San Jacinto battle-ground

From: History of Texas from its first settlement in 1685 to its joining the United States on December 29, 1845 / H. Yoakum

History of Texas: From Its First Settlement in 1685 to Its Annexation to the United States in 1846, Volume 1

Book describing Texas history up to the time of annexation to the United States of America. This first volume is broken into 22 chapters covering the start of European immigration (roughly 1685) through the establishment of the Republic of Texas in 1835, with a number of appendices containing supplementary information

History of Texas: From Its First Settlement in 1685 to Its Annexation to the United States in 1846, Volume 2

Book describing Texas history; this second volume is broken into 14 chapters covering the start of the Republic of Texas in 1835 through annexation by the U.S. in 1846, with a number of appendices containing supplementary information