SAR Ship Target Recognition Via Multi-Scale Feature Attention and Adaptive-Weighed Classifier

Maritime surveillance is indispensable for civilian fields, including national maritime safeguarding, channel monitoring, and so on, in which synthetic aperture radar (SAR) ship target recognition is a crucial research field. The core problem to realizing accurate SAR ship target recognition is the large inner-class variance and inter-class overlap of SAR ship features, which limits the recognition performance. Most existing methods plainly extract multi-scale features of the network and utilize equally each feature scale in the classification stage. However, the shallow multi-scale features are not discriminative enough, and each scale feature is not equally effective for recognition. These factors lead to the limitation of recognition performance. Therefore, we proposed a SAR ship recognition method via multi-scale feature attention and adaptive-weighted classifier to enhance features in each scale, and adaptively choose the effective feature scale for accurate recognition. We first construct an in-network feature pyramid to extract multi-scale features from SAR ship images. Then, the multi-scale feature attention can extract and enhance the principal components from the multi-scale features with more inner-class compactness and inter-class separability. Finally, the adaptive weighted classifier chooses the effective feature scales in the feature pyramid to achieve the final precise recognition. Through experiments and comparisons under OpenSARship data set, the proposed method is validated to achieve state-of-the-art performance for SAR ship recognition

Boosting Multi-Modal E-commerce Attribute Value Extraction via Unified Learning Scheme and Dynamic Range Minimization

With the prosperity of e-commerce industry, various modalities, e.g., vision and language, are utilized to describe product items. It is an enormous challenge to understand such diversified data, especially via extracting the attribute-value pairs in text sequences with the aid of helpful image regions. Although a series of previous works have been dedicated to this task, there remain seldomly investigated obstacles that hinder further improvements: 1) Parameters from up-stream single-modal pretraining are inadequately applied, without proper jointly fine-tuning in a down-stream multi-modal task. 2) To select descriptive parts of images, a simple late fusion is widely applied, regardless of priori knowledge that language-related information should be encoded into a common linguistic embedding space by stronger encoders. 3) Due to diversity across products, their attribute sets tend to vary greatly, but current approaches predict with an unnecessary maximal range and lead to more potential false positives. To address these issues, we propose in this paper a novel approach to boost multi-modal e-commerce attribute value extraction via unified learning scheme and dynamic range minimization: 1) Firstly, a unified scheme is designed to jointly train a multi-modal task with pretrained single-modal parameters. 2) Secondly, a text-guided information range minimization method is proposed to adaptively encode descriptive parts of each modality into an identical space with a powerful pretrained linguistic model. 3) Moreover, a prototype-guided attribute range minimization method is proposed to first determine the proper attribute set of the current product, and then select prototypes to guide the prediction of the chosen attributes. Experiments on the popular multi-modal e-commerce benchmarks show that our approach achieves superior performance over the other state-of-the-art techniques

Identification and Characterization of an Efficient acyl-CoA:Diacylglycerol Acyltransferase 1 (\u3cem\u3eDGAT1\u3c/em\u3e) Gene from the Microalga \u3cem\u3eChlorella ellipsoidea\u3c/em\u3e

Background: Oil in the form of triacylglycerols (TAGs) is quantitatively the most important storage form of energy for eukaryotic cells. Diacylglycerol acyltransferase (DGAT) is considered the rate-limiting enzyme for TAG accumulation. Chlorella, a unicellular eukaryotic green alga, has attracted much attention as a potential feedstock for renewable energy production. However, the function of DGAT1 in Chlorella has not been reported. Results: A full-length cDNA encoding a putative diacylglycerol acyltransferase 1 (DGAT1, EC 2.3.1.20) was obtained from Chlorella ellipsoidea. The 2,142 bp open reading frame of this cDNA, designated CeDGAT1, encodes a protein of 713 amino acids showing no more than 40% identity with DGAT1s of higher plants. Transcript analysis showed that the expression level of CeDGAT1 markedly increased under nitrogen starvation, which led to significant triacylglycerol (TAG) accumulation. CeDGAT1 activity was confirmed in the yeast quadruple mutant strain H1246 by restoring its ability to produce TAG. Upon expression of CeDGAT1, the total fatty acid content in wild-type yeast (INVSc1) increased by 142%, significantly higher than that transformed with DGAT1s from higher plants, including even the oil crop soybean. The over-expression of CeDGAT1 under the NOS promoter in wild-type Arabidopsis thaliana and Brassica napus var. Westar significantly increased the oil content by 8–37% and 12–18% and the average 1,000-seed weight by 9–15% and 6–29%, respectively, but did not alter the fatty acid composition of the seed oil. The net increase in the 1,000-seed total lipid content was up to 25–50% in both transgenic Arabidopsis and B. napus. Conclusions: We identified a gene encoding DGAT1 in C. ellipsoidea and confirmed that it plays an important role in TAG accumulation. This is the first functional analysis of DGAT1 in Chlorella. This information is important for understanding lipid synthesis and accumulation in Chlorella and for genetic engineering to enhance oil production in microalgae and oil plants

Identification and characterization of an efficient acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) gene from the microalga Chlorella ellipsoidea

RT-PCR detection of DGAT1 genes in transgenic yeast (INVSc1). The yeast actin was used as an internal control. 1, The yeast transformed with pYES2.0; 2–5, the yeast expressing AtDGAT1, GmDGAT1, BnDGAT1 and CeDGAT1, respectively. (DOCX 55 kb

Extraction Optimization of Water-Extracted Mycelial Polysaccharide from Endophytic Fungus Fusarium oxysporum Dzf17 by Response Surface Methodology

Water-extracted mycelial polysaccharide (WPS) from the endophytic fungus Fusarium oxysporum Dzf17 isolated from Dioscorea zingiberensis was found to be an efficient elicitor to enhance diosgenin accumulation in D. zingigerensis cultures, and also demonstrated antioxidant activity. In this study, response surface methodology (RSM) was employed to optimize the extraction process of WPS from F. oxysporum Dzf17 using Box-Behnken design (BBD). The ranges of the factors investigated were 1–3 h for extraction time (X1), 80–100 °C for extraction temperature (X2), and 20–40 (v/w) for ratio of water volume (mL) to raw material weight (g) (X3). The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis. Statistical analysis showed that the polynomial regression model was in good agreement with the experimental results with the determination coefficient (R2) of 0.9978. By solving the regression equation and analyzing the response surface contour plots, the extraction parameters were optimized as 1.7 h for extraction time, 95 °C for extraction temperature, 39 (v/w) for ratio of water volume (mL) to raw material weight (g), and with 2 extractions. The maximum value (10.862%) of WPS yield was obtained when the WPS extraction process was conducted under the optimal conditions

Positron Emission Tomography Imaging of CD105 Expression with a 64Cu-Labeled Monoclonal Antibody: NOTA Is Superior to DOTA

Optimizing the in vivo stability of positron emission tomography (PET) tracers is of critical importance to cancer diagnosis. In the case of 64Cu-labeled monoclonal antibodies (mAb), in vivo behavior and biodistribution is critically dependent on the performance of the bifunctional chelator used to conjugate the mAb to the radiolabel. This study compared the in vivo characteristics of 64Cu-labeled TRC105 (a chimeric mAb that binds to both human and murine CD105), through two commonly used chelators: 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) and 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA). Flow cytometry analysis confirmed that chelator conjugation of TRC105 did not affect its CD105 binding affinity or specificity. PET imaging and biodistribution studies in 4T1 murine breast tumor-bearing mice revealed that 64Cu-NOTA-TRC105 exhibited better stability than 64Cu-DOTA-TRC105 in vivo, which resulted in significantly lower liver uptake without compromising the tumor targeting efficiency. In conclusion, this study confirmed that NOTA is a superior chelator to DOTA for PET imaging with 64Cu-labeled TRC105