Biogenic gas nanostructures as ultrasonic molecular reporters.

Ultrasound is among the most widely used non-invasive imaging modalities in biomedicine, but plays a surprisingly small role in molecular imaging due to a lack of suitable molecular reporters on the nanoscale. Here, we introduce a new class of reporters for ultrasound based on genetically encoded gas nanostructures from microorganisms, including bacteria and archaea. Gas vesicles are gas-filled protein-shelled compartments with typical widths of 45-250 nm and lengths of 100-600 nm that exclude water and are permeable to gas. We show that gas vesicles produce stable ultrasound contrast that is readily detected in vitro and in vivo, that their genetically encoded physical properties enable multiple modes of imaging, and that contrast enhancement through aggregation permits their use as molecular biosensors

A state-of-the-art overview of job-crafting research:Current trends and future research directions

Purpose: In celebration of the 25th anniversary of the founding of Career Development International, a state-of-the-art overview of recent trends in job-crafting research was conducted. Since job crafting was introduced twenty years ago as a type of proactive work behavior that employees engage in to adjust their jobs to their needs, skills, and preferences, research has evolved tremendously. Design/methodology/approach: To take stock of recent developments and to unravel the latest trends in the field, this overview encompasses job-crafting research published in the years 2016–2021. The overview portrays that recent contributions have matured the theoretical and empirical advancement of job-crafting research from three perspectives (i.e. individual, team and social). Findings: When looking at the job-crafting literature through these three perspectives, a total of six trends were uncovered that show that job-crafting research has moved to a more in-depth theory-testing approach; broadened its scope; examined team-level job crafting and social relationships; and focused on the impact of job crafting on others in the work environment and their evaluations and reactions to it. Originality/value: The overview of recent trends within the job-crafting literature ends with a set of recommendations for how future research on job crafting could progress and create scientific impact for the coming years

The effects of glycyrrhizic acid and glabridin in the regulation of CXCL5 inflammation gene on acceleration of wound healing

AbstractObjectiveTo evaluate the anti-inflammatory property of both glycyrrhizic acid (GA) and glabridin in reducing inflammation to accelerate wound regeneration on 3T3-L1 and NIH-3T3 fibroblast cell lines.MethodsCell proliferation and viability assay (MTT assay), scratch wound healing assays, and quantitative real-time PCR were conducted to investigate the effects on cell proliferation, cell migration, and expression of CXC chemokine ligand 5 inflammation gene respectively.ResultsResults showed that at a low concentration of 1 × 10−8 mol/L, glabridin down regulated cell proliferation in NIH-3T3 significantly, suggesting its involvement in ERK1/2 signaling pathway. GA and glabridin significantly accelerated cell migration through wound healing in both 3T3-L1 and NIH-3T3 and significantly down regulated the expression of CXC chemokine ligand 5 in 3T3-L1 at concentration 1 × 10−8 mol/L, indicating the possible involvement of nuclear factor-κB and cyclooxygenase 2 transcriptions modulation.ConclusionsBoth GA and glabridin can serve as potential treatment for chronic inflammatory disease, and glabridin as an oncogenic inhibitor due to its anti-proliferative property

Cultural Adaptation of a Substance Abuse Prevention Program as a Catalyst for Community Change

The aim of the current paper is to discuss the use of Outcome Mapping as a tool for evaluating community and stakeholder changes that occurred when a prevention program was culturally adapted and delivered through a community-university partnership. To the authors’ knowledge, this paper represents the first account of using Outcome Mapping as an evaluation tool in a Canadian Indigenous context. Changes in the behavior, actions, activities, and relationships of five boundary partners were retrospectively documented using the tool. Data demonstrated positive impact on Elders and students; growing community investment in and support for the Maskwacis Life Skills Training program’s cultural components; progressive increases in community ownership of the program; and growth in the community-university partnership. Overall, Outcome Mapping provided a systematic method for understanding peripheral changes that are often overlooked in conventional research and evaluation, but that nonetheless indicate progress toward community changes and long-term impact

Old Ways for New Days

This Open Access book provides a critical reflection into how indigenous cultures are attempting to adapt to climate change. Through detailed first-hand accounts, the book describes the unique challenges facing indigenous peoples in the context of climate change adaptation, governance, communication strategies, and institutional pressures. The book shows how current climate change terminologies and communication strategies often perpetuate the marginalisation of indigenous peoples and suggests that new approaches that prioritise Indigenous voices, agency and survival are required. The book first introduces readers to Indigenous peoples and their struggles related to climate change, describing the impacts of climate change on their everyday lives and the adaptation strategies currently undertaken to address them. These strategies are then detailed through case studies which focus on how Indigenous knowledge and practices have been used to respond to and cope with climate change in a variety of environments, including urban settings. The book discusses specific governance challenges facing Indigenous peoples, and presents new methods for engagement that will bridge existing communication gaps to ensure Indigenous peoples are central to the implementation of climate change adaptation measures. This book is intended for an audience of Indigenous peoples, adaptation practitioners, academics, students, policy makers and government workers. ; Documents for the first time how some of the world's oldest living indigenous cultures are attempting to adapt to climate change Summarizes key issues facing indigenous peoples in the context of adaptation to climate change impacts Offers critical reflection on specific governance challenges faced by indigenous people

OMMA enables population-scale analysis of complex genomic features and phylogenomic relationships from nanochannel-based optical maps.

BackgroundOptical mapping is an emerging technology that complements sequencing-based methods in genome analysis. It is widely used in improving genome assemblies and detecting structural variations by providing information over much longer (up to 1 Mb) reads. Current standards in optical mapping analysis involve assembling optical maps into contigs and aligning them to a reference, which is limited to pairwise comparison and becomes bias-prone when analyzing multiple samples.FindingsWe present a new method, OMMA, that extends optical mapping to the study of complex genomic features by simultaneously interrogating optical maps across many samples in a reference-independent manner. OMMA captures and characterizes complex genomic features, e.g., multiple haplotypes, copy number variations, and subtelomeric structures when applied to 154 human samples across the 26 populations sequenced in the 1000 Genomes Project. For small genomes such as pathogenic bacteria, OMMA accurately reconstructs the phylogenomic relationships and identifies functional elements across 21 Acinetobacter baumannii strains.ConclusionsWith the increasing data throughput of optical mapping system, the use of this technology in comparative genome analysis across many samples will become feasible. OMMA is a timely solution that can address such computational need. The OMMA software is available at https://github.com/TF-Chan-Lab/OMTools

A Numerical Investigation of Hurricane Florence-Induced Compound Flooding in the Cape Fear Estuary Using a Dynamically Coupled Hydrological-Ocean Model

Hurricane-induced compound flooding is a combined result of multiple processes, including overland runoff, precipitation, and storm surge. This study presents a dynamical coupling method applied at the boundary of a processes-based hydrological model (the hydrological modeling extension package of the Weather Research and Forecasting model) and the two-dimensional Regional Ocean Modeling System on the platform of the Coupled-Ocean-Atmosphere-Wave-Sediment Transport Modeling System. The coupled model was adapted to the Cape Fear River Basin and adjacent coastal ocean in North Carolina, United States, which suffered severe losses due to the compound flood induced by Hurricane Florence in 2018. The model\u27s robustness was evaluated via comparison against observed water levels in the watershed, estuary, and along the coast. With a series of sensitivity experiments, the contributions from different processes to the water level variations in the estuary were untangled and quantified. Based on the temporal evolution of wind, water flux, water level, and water-level gradient, compound flooding in the estuary was categorized into four stages: (I) swelling, (II) local-wind-dominated, (III) transition, and (IV) overland-runoff-dominated. A nonlinear effect was identified between overland runoff and water level in the estuary, which indicated the estuary could serve as a buffer for surges from the ocean side by reducing the maximum surge height. Water budget analysis indicated that water in the estuary was flushed 10 times by overland runoff within 23 days after Florence\u27s landfall

Old Ways for New Days

This Open Access book provides a critical reflection into how indigenous cultures are attempting to adapt to climate change. Through detailed first-hand accounts, the book describes the unique challenges facing indigenous peoples in the context of climate change adaptation, governance, communication strategies, and institutional pressures. The book shows how current climate change terminologies and communication strategies often perpetuate the marginalisation of indigenous peoples and suggests that new approaches that prioritise Indigenous voices, agency and survival are required. The book first introduces readers to Indigenous peoples and their struggles related to climate change, describing the impacts of climate change on their everyday lives and the adaptation strategies currently undertaken to address them. These strategies are then detailed through case studies which focus on how Indigenous knowledge and practices have been used to respond to and cope with climate change in a variety of environments, including urban settings. The book discusses specific governance challenges facing Indigenous peoples, and presents new methods for engagement that will bridge existing communication gaps to ensure Indigenous peoples are central to the implementation of climate change adaptation measures. This book is intended for an audience of Indigenous peoples, adaptation practitioners, academics, students, policy makers and government workers. ; Documents for the first time how some of the world's oldest living indigenous cultures are attempting to adapt to climate change Summarizes key issues facing indigenous peoples in the context of adaptation to climate change impacts Offers critical reflection on specific governance challenges faced by indigenous people

A Community-University Approach to Substance Abuse Prevention

In response to high rates of substance abuse in their communities, members of the Maskwacis four Nations invited university researchers to partner in culturally adapting, implementing, and evaluating an evidence-based substance abuse and violence prevention program, the Life Skills Training program (Botvin & Griffin, 2014). This project used a community-based participatory research (Israel, Schulz, Parker, & Becker, 1998; Minkler & Wallerstein, 2003) approach, and was carried out by university and First Nation community partners. To evaluate the impact of the adapted program, students completed pre and post questionnaires, and community members participated in focus groups. The adapted Maskwacis Life Skills Training program was delivered in schools for three years. Students’ knowledge increased significantly during program delivery, and strong support was documented from community members. This project demonstrates the impact that can be facilitated by culturally adapting and delivering a prevention program, and by forming a community-university partnership