Antimicrobial Activity of Phomopsis SP. ED2, an Endophytic Fungus Isolated From Orthosiphon Stamineus Benth

This study aimed to investigate the antimicrobial activity of the endophytic fungus Phomopsis sp. ED2, which was previously isolated from the flower of the medicinal plant Orthosiphon stamineus Benth, in Penang, Malaysia. The 14 days-old fungal cultures were extracted with different organic solvents, include hexane, dichloromethane, ethyl acetate and butanol. The antimicrobial activities of the extracts were tested by disc diffusion assay. Based on the results, most antimicrobial compounds were present in the ethyl acetate fraction, and this extract could significantly inhibit the activity of bacteria, yeasts and fungi. The results also indicate that the antimicrobial compounds were mainly associated with the fungal biomass and all the Gram-positive test bacteria were inhibited by the ethyl acetate extract of the fungal biomass. Notably, methicillin-resistant Staphylococcus aereus exhibited a high sensitivity to the extract, with a low minimal inhibitory concentration and low minimal lethal concentration. The isolate also effectively inhibited the growth of the dermatophytes Microsporum gypseum and Trichophyton rubrum. Preliminary phytochemical screening also demonstrated that the extract contains phenols and steroids which possess antimicrobial activity. These results reveal that the endophytic fungus Phomopsis sp. is potential source of novel chemotherapeutic agent

Anti-MRSA Activity of Penicillium Minioluteum ED24, an Endophytic Fungus Isolated From Orthosiphon Stamineus Benth

Nowadays, the medical concerns with Methicillin-resistant Staphylococcus aereus (MRSA) arised when in 2009, the proportion of S. aureus isolates that are resistant to methicilin has increased to 59.5%- 64.4% in South East Asia. Hence a new antibacterial agent from natural source is necessary to combat the infectious diseases. This study aimed to investigate the anti-MRSA activity of the endophytic fungus Penicillium minioluteum ED24, which was previously isolated from the leaf of the medicinal plant Orthosiphon stamineus Benth, in Penang, Malaysia. Methanol was used to extract the freeze-dried fungal biomass of the 14-days old fungal culture. The extract showed very significant anti-MRSA activity of disc diffusion assay with the minimal inhibitory concentration of 31.3 mg/mL and minimal lethality concentration of 250 mg/mL. Besides, 50% growth reduction of MRSA was observed at 33.2 h at the concentration of extract at MIC and 26.7 h at concentration of 2MIC. The structural degeneration of MRSA was observed by using scanning electron microscope (SEM). The SEM micrographs showed that the formation of cavities were observe on the extract treated cells and the cell wall structure of the MRSA was collapsed after treated with the fungal extract. The results suggesting that the bacterial cell wall is the target of the antibiotic compound(s) present in the extract. These results reveal that the endophytic fungus P. minioluteum ED24 a is potential source of anti-MRSA compound

Aerodynamic control of NASP-type vehicles through Vortex manipulation. Volume 1: Static water tunnel tests

Water tunnel tests were conducted on a NASP-type configuration to evaluate different pneumatic Forebody Vortex Control (FVC) methods. Flow visualization and yawing moment measurements were performed at angles of attack from 0 deg to 30 deg. The pneumatic techniques tested included jet and slot blowing. In general, blowing can be used efficiently to manipulate the forebody vortices at angles of attack greater than 20 deg. These vortices are naturally symmetric up to alpha = 25 deg and asymmetric between 25 deg and 30 deg angle of attack. Results indicate that tangential aft jet blowing is the most promising method for this configuration. Aft jet blowing produces a yawing moment towards the blowing side and the trends with blowing rate are well behaved. The size of the nozzle is not the dominant factor in the blowing process; the change in the blowing 'momentum,' i.e., the product of the mass flow rate and the velocity of the jet, appears to be the important parameter in the water tunnel (incompressible and unchoked flow at the nozzle exit). Forward jet blowing is very unpredictable and sensitive to mass flow rate changes. Slot blowing (with the exception of very low blowing rates) acts as a flow 'separator'; it promotes early separation on the blow side, producing a yawing moment toward the non-blowing side for the C(sub mu) range investigated

Aerodynamic control of NASP-type vehicles through vortex manipulation. Volume 3: Wing rock experiments

Free-to-roll tests were conducted in water and wind tunnels in an effort to investigate the mechanisms of wing rock on a NASP-type vehicle. The configuration tested consisted of a highly-slender forebody and a 78 deg swept delta wing. In the water tunnel test, extensive flow visualization was performed and roll angle histories were obtained. In the wind tunnel test, the roll angle, forces and moments, and limited forebody and wing surface pressures were measured during the wing rock motion. A limit cycle oscillation was observed for angles of attack between 22 deg and 30 deg. In general, the experiments confirmed that the main flow phenomena responsible for the wing-body-tail wing rock are the interactions between the forebody and the wing vortices. The variation of roll acceleration (determined from the second derivative of the roll angle time history) with roll angle clearly slowed the energy balance necessary to sustain the limit cycle oscillation. Different means of suppressing wing rock by controlling the forebody vortices using small blowing jets were also explored. Steady blowing was found to be capable of suppressing wing rock, but significant vortex asymmetrices are created, causing the model to stop at a non-zero roll angle. On the other hand, alternating pulsed blowing on the left and right sides of the fore body was demonstrated to be a potentially effective means of suppressing wing rock and eliminating large asymmetric moments at high angles of attack

European Heart Rhythm Association (EHRA)/Heart Rhythm Society (HRS)/Asia Pacific Heart Rhythm Society (APHRS)/Latin American Heart Rhythm Society (LAHRS) expert consensus on risk assessment in cardiac arrhythmias: use the right tool for the right outcome, in the right population.

In clinical practice and for scientific purposes, cardiologists and primary care physicians perform risk assessment in patients with cardiac diseases or conditions with high risk of developing such. The European Heart Rhythm Association (EHRA), Heart Rhythm Society (HRS), Asia Pacific Heart Rhythm Society (APHRS), and the Latin American Heart Rhythm Society (LAHRS) set down this expert consensus statement task force to summarize the consensus regarding risk assessment in cardiac arrhythmias. Objectives were to raise awareness of using the right risk assessment tool for a given outcome in a given population, and to provide physicians with practical proposals that may lead to rational and evidence-based risk assessment and improvement of patient care in this regard. A large variety of methods are used for risk assessment and choosing the best methods and tools hereof in a given situation is not simple. Even though parameters and test results found associated with increased risk of one outcome (e.g. death) may also be associated with higher risk of other adverse outcomes, specific risk assessment strategies should be used only for the purposes for which they are validated. The work of this task force is summarized in a row of consensus statement tables

The Engineering Design of Man-Machine Interface for RTS

The purpose of this paper is to present the engineering design of the advanced Man-Machine Interface (MMI) of the Integrated system for Radwaste Treatment and Storage (RTS) facility in Institute of Nuclear Energy Research (INER) Taiwan, ROC. To build the RTS, a multi-function radwaste facility with a total storage of about 10,000 drums, is a five-year project starting in 2000 including intermediate activity waste treatment and combustible waste storage. The completed engineering design of the MMI is based on proven technologies and digital control systems, enhancing the radwaste management efficiency and reliability of operator's performance as well as assuring the dose exposure of personnel meeting the regulation standard. Over past few years, INER has accumulated extensive experience in the area of radwaste treatment and storage. Therefore, we are confident that we will complete this project with fulfillment of the requirements of RTS

The Assessment of Climate Change Impacts and Land-use Changes on Flood Characteristics: The Case Study of the Kelani River Basin, Sri Lanka

Understanding the changes in climate and land use/land cover (LULC) over time is important for developing policies for minimizing the socio-economic impacts of riverine floods. The present study evaluates the influence of hydro-climatic factors and anthropogenic practices related to LULC on floods in the Kelani River Basin (KRB) in Sri Lanka. The gauge-based daily precipitation, monthly mean temperature, daily discharges, and water levels at sub-basin/basin outlets, and both surveyed and remotely sensed inundation areas were used for this analysis. Flood characteristics in terms of mean, maximum, and number of peaks were estimated by applying the peak over threshold (POT) method. Nonparametric tests were also used to identify the climatic trends. In addition, LULC maps were generated over the years 1988–2017 using Landsat images. It is observed that the flood intensities and frequencies in the KRB have increased over the years. However, Deraniyagala and Norwood sub-basins have converted to dry due to the decrease in precipitation, whereas Kithulgala, Holombuwa, Glencourse, and Hanwella showed an increase in precipitation. A significant variation in atmospheric temperature was not observed. Furthermore, the LULC has mostly changed from vegetation/barren land to built-up in many parts of the basin. Simple correlation and partial correlation analysis showed that flood frequency and inundation areas have a significant correlation with LULC and hydro-climatic factors, especially precipitation over time. The results of this research will therefore be useful for policy makers and environmental specialists to understand the relationship of flood frequencies with the anthropogenic influences on LULC and climatic factors.publishedVersio