7 research outputs found
Signal transduction in light-oxygen-voltage receptors lacking the adduct- forming cysteine residue
Light–oxygen–voltage (LOV) receptors sense blue light through the
photochemical generation of a covalent adduct between a flavin-nucleotide
chromophore and a strictly conserved cysteine residue. Here we show that,
after cysteine removal, the circadian-clock LOV-protein Vivid still undergoes
light-induced dimerization and signalling because of flavin photoreduction to
the neutral semiquinone (NSQ). Similarly, photoreduction of the engineered LOV
histidine kinase YF1 to the NSQ modulates activity and downstream effects on
gene expression. Signal transduction in both proteins hence hinges on flavin
protonation, which is common to both the cysteinyl adduct and the NSQ. This
general mechanism is also conserved by natural cysteine-less, LOV-like
regulators that respond to chemical or photoreduction of their flavin
cofactors. As LOV proteins can react to light even when devoid of the adduct-
forming cysteine, modern LOV photoreceptors may have arisen from ancestral
redox-active flavoproteins. The ability to tune LOV reactivity through
photoreduction may have important implications for LOV mechanism and
optogenetic applications
Peripheral Methionine Residues Impact Flavin Photoreduction and Protonation in an Engineered LOV Domain Light Sensor
Yee EF, Oldemeyer S, Böhm E, et al. Peripheral Methionine Residues Impact Flavin Photoreduction and Protonation in an Engineered LOV Domain Light Sensor. Biochemistry. 2021;60(15):1148-1164
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Zng1 is a GTP-dependent zinc transferase needed for activation of methionine aminopeptidase
The evolution of zinc (Zn) as a protein cofactor altered the functional landscape of biology, but dependency on Zn also created an Achilles' heel, necessitating adaptive mechanisms to ensure Zn availability to proteins. A debated strategy is whether metallochaperones exist to prioritize essential Zn-dependent proteins. Here, we present evidence for a conserved family of putative metal transferases in human and fungi, which interact with Zn-dependent methionine aminopeptidase type I (MetAP1/Map1p/Fma1). Deletion of the putative metal transferase in Saccharomyces cerevisiae (ZNG1; formerly YNR029c) leads to defective Map1p function and a Zn-deficiency growth defect. In vitro, Zng1p can transfer Zn2+ or Co2+ to apo-Map1p, but unlike characterized copper chaperones, transfer is dependent on GTP hydrolysis. Proteomics reveal mis-regulation of the Zap1p transcription factor regulon because of loss of ZNG1 and Map1p activity, suggesting that Zng1p is required to avoid a compounding effect of Map1p dysfunction on survival during Zn limitation
Patching a leak in an R1 university gateway STEM course.
A cognitively intensive companion service course has been introduced to the main fall general chemistry class at Cornell University. For years 2015 and 2016, priority students (those from groups under-represented and economically disadvantaged) show respectively improvement of +0.67 and +0.51 standard deviations in final course grade compared to priority students not in the program. Non-priority students show respectively a +0.66 and +0.62 standard deviation improvement. Progressive improvement (as measured by higher than expected Final Exam scores than what would have been expected solely from a given student's earlier Exam 1 score) demonstrates conclusively the service course's role in the enhanced outcomes. Progressive retention (as measured by the following year fall semester's organic chemistry exam scores compared to what would have been expected based on a given student's general chemistry final exam score) demonstrates that, on the average, the earlier observed progressive improvement is significantly retained in a chemistry course one year later. Preliminary retention statistics suggest a significant increase in first year to second year retention. A meta analysis of results from previously reported chemistry service courses indicate that such performance gains are difficult to achieve and hence common elements of the few effective programs may be of high value to the STEM education community
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A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria
Heme has a critical role in the chemical framework of the cell as an essential protein cofactor and signaling molecule that controls diverse processes and molecular interactions. Using a phylogenomics-based approach and complementary structural techniques, we identify a family of dimeric hemoproteins comprising a domain of unknown function DUF2470. The heme iron is axially coordinated by two zinc-bound histidine residues, forming a distinct two-fold symmetric zinc-histidine-iron-histidine-zinc site. Together with structure-guided in vitro and in vivo experiments, we further demonstrate the existence of a functional link between heme binding by Dri1 (Domain related to iron 1, formerly ssr1698) and post-translational regulation of succinate dehydrogenase in the cyanobacterium Synechocystis, suggesting an iron-dependent regulatory link between photosynthesis and respiration. Given the ubiquity of proteins containing homologous domains and connections to heme metabolism across eukaryotes and prokaryotes, we propose that DRI (Domain Related to Iron; formerly DUF2470) functions at the molecular level as a heme-dependent regulatory domain