Studies of interface damping Summary technical report, 25 Aug. 1967 - 25 Mar. 1968

Air flow and vacuum effects on damping between solid-solid sliding interfaces in aluminum space structures in relation to surface oxide layer

Matrix and Tensor Factorization Methods for Toxicogenomic Modeling and Prediction

Peer reviewe

Negative Regulation of Active Zone Assembly by a Newly Identified SR Protein Kinase

A neuronal serine-arginine protein kinase that localizes to the presynaptic active zone is required for kinase-dependent repression of active zone assembly

A Global View of Cancer-Specific Transcript Variants by Subtractive Transcriptome-Wide Analysis

BACKGROUND: Alternative pre-mRNA splicing (AS) plays a central role in generating complex proteomes and influences development and disease. However, the regulation and etiology of AS in human tumorigenesis is not well understood. METHODOLOGY/PRINCIPAL FINDINGS: A Basic Local Alignment Search Tool database was constructed for the expressed sequence tags (ESTs) from all available databases of human cancer and normal tissues. An insertion or deletion in the alignment of EST/EST was used to identify alternatively spliced transcripts. Alignment of the ESTs with the genomic sequence was further used to confirm AS. Alternatively spliced transcripts in each tissue were then subtractively cross-screened to obtain tissue-specific variants. We systematically identified and characterized cancer/tissue-specific and alternatively spliced variants in the human genome based on a global view. We identified 15,093 cancer-specific variants of 9,989 genes from 27 types of human cancers and 14,376 normal tissue-specific variants of 7,240 genes from 35 normal tissues, which cover the main types of human tumors and normal tissues. Approximately 70% of these transcripts are novel. These data were integrated into a database HCSAS (http://202.114.72.39/database/human.html, pass:68756253). Moreover, we observed that the cancer-specific AS of both oncogenes and tumor suppressor genes are associated with specific cancer types. Cancer shows a preference in the selection of alternative splice-sites and utilization of alternative splicing types. CONCLUSIONS/SIGNIFICANCE: These features of human cancer, together with the discovery of huge numbers of novel splice forms for cancer-associated genes, suggest an important and global role of cancer-specific AS during human tumorigenesis. We advise the use of cancer-specific alternative splicing as a potential source of new diagnostic, prognostic, predictive, and therapeutic tools for human cancer. The global view of cancer-specific AS is not only useful for exploring the complexity of the cancer transcriptome but also widens the eyeshot of clinical research

Soil Moisture Gradients and Controls on a Southern Appalachian Hillslope from Drought Through Recharge

Soil moisture gradients along hillslopes in humid watersheds, although indicated by vegetation gradients and by studies using models, have been difficult to confirm empirically. While soil properties and topographic features are the two general physio-graphic factors controlling soil moisture on hillslopes, studies have shown conflicting results regarding which factor is more important. The relative importance of topographic and soil property controls was examined in an upland forested watershed at the Coweeta Hydrologic Laboratory in the southern Appalachian mountains. Soil moisture was measured along a hillslope transect with a mesic-to-xeric forest vegetation gradient over a period spanning precipitation extremes. The hillslope was transect instrumented with a time domain reflectometry (TDR) network at two depths. Soil moisture was measured during a severe autumn drought and subsequent winter precipitation recharge. In the upper soil depth (0-30 cm), moisture gradients persisted throughout the measurement period, and topography exerted dominant control. For the entire root zone (0-90 cm), soil moisture gradients were found only during drought. Control on soil moisture was due to both topography and storage before drought. During and after recharge, variations in soil texture and horizon distribution exerted dominant control on soil moisture content in the root zone (0-90 cm). These results indicate that topographic factors assert more control over hillslope soil moisture during drier periods as drainage progresses, while variations in soil water storage properties are more important during wetter periods. Hillslope soil moisture gradients in southern Appalachian watersheds appear to be restricted to upper soil layers, with deeper hillslope soil moisture gradients occurring only with sufficient drought

Hillslope Nutrient Dynamics Following Upland Riparian Vegetation Disturbance

We investigated the effects of removing near-stream Rhododendron and of the natural blowdown of canopy trees on nutrient export to streams in the southern Appalachians. Transects were instrumented on adjacent hillslopes in a first-order watershed at the Coweeta Hydrologic Laboratory (35°03′N, 83°25′W). Dissolved organic carbon (DOC), K+, Na+, Ca2+, Mg2+, NO3-- -N, NH4+ -N, PO43---P, and SO42- were measured for 2 years prior to disturbance. In August 1995, riparian Rhododendron on one hillslope was cut, removing 30% of total woody biomass. In October 1995, Hurricane Opal uprooted nine canopy trees on the other hillslope, downing 81% of the total woody biomass. Over the 3 years following the disturbance, soilwater concentrations of NO3-- -N tripled on the cut hillslope. There were also small changes in soilwater DOC, SO42-, Ca2+, and Mg2+. However, no significant changes occurred in groundwater nutrient concentrations following Rhododendron removal. In contrast, soilwater NO3-- -N on the storm-affected hillslope showed persistent 500-fold increases, groundwater NO3-- -N increased four fold, and streamwater NO3-- -N doubled. Significant changes also occurred in soilwater pH, DOC, SO42-, Ca2+, and Mg2+. There were no significant changes in microbial immobilization of soil nutrients or water outflow on the storm-affected hillslope. Our results suggest that Rhododendron thickets play a relatively minor role in controlling nutrient export to headwater streams. They further suggest that nutrient uptake by canopy trees is a key control on NO3-- -N export in upland riparian zones, and that disruption of the root–soil connection in canopy trees via uprooting promotes significant nutrient loss to streams