63 research outputs found
Effects of Delayed Finishing/Polishing on Surface Roughness, Hardness and Gloss of Tooth-Coloured Restorative Materials
Objectives: The aim of this study was to investigate the effect of delayed finishing/polishing on the surface roughness, hardness and gloss of tooth-coloured restorative materials. Methods: Four different tooth-coloured restoratives: a flowable resin composite- Tetric Flow, a hybrid resin composite- Venus, a nanohybrid resin composite- Grandio, and a polyacid modified resin composite- Dyract Extra were used. 30 specimens were made for each material and randomly assigned into three groups. The first group was finished/polished immediately and the second group was finished/polished after 24 hours. The remaining 10 specimens served as control. The surface roughness of each sample was recorded using a laser profilometer. Gloss measurements were performed using a small-area glossmeter. Vickers microhardness measurements were performed from three locations on each specimen surface under 100g load and 10s dwell time. Data for surface roughness and hardness were analyzed by Kruskal Wallis test and data for gloss were subjected to one-way ANOVA and Tukey test (P .05). The lowest hardness values were found under Mylar strip. Delayed finishing/polishing significantly increased the hardness of all materials. Conclusions: The effect of delayed finishing/polishing on surface roughness, gloss and hardness appears to be material dependent.PubMe
Nature of the spin resonance mode in CeCoIn
Spin-fluctuation-mediated unconventional superconductivity can emerge at the
border of magnetism, featuring a superconducting order parameter that changes
sign in momentum space. Detection of such a sign-change is experimentally
challenging, since most probes are not phase-sensitive. The observation of a
spin resonance mode (SRM) from inelastic neutron scattering is often seen as
strong phase-sensitive evidence for a sign-changing superconducting order
parameter, by assuming the SRM is a spin-excitonic bound state. Here, we show
that for the heavy fermion superconductor CeCoIn, its SRM defies
expectations for a spin-excitonic bound state, and is not a manifestation of
sign-changing superconductivity. Instead, the SRM in CeCoIn likely arises
from a reduction of damping to a magnon-like mode in the superconducting state,
due to its proximity to magnetic quantum criticality. Our findings emphasize
the need for more stringent tests of whether SRMs are spin-excitonic, when
using their presence to evidence sign-changing superconductivity.Comment: accepted for publication in Communications Physic
Robust Upward Dispersion of the Neutron Spin Resonance in the Heavy Fermion Superconductor CeYbCoIn
The neutron spin resonance is a collective magnetic excitation that appears
in copper oxide, iron pnictide, and heavy fermion unconventional
superconductors. Although the resonance is commonly associated with a
spin-exciton due to the ()-wave symmetry of the superconducting
order parameter, it has also been proposed to be a magnon-like excitation
appearing in the superconducting state. Here we use inelastic neutron
scattering to demonstrate that the resonance in the heavy fermion
superconductor CeYbCoIn with has a ring-like
upward dispersion that is robust against Yb-doping. By comparing our
experimental data with random phase approximation calculation using the
electronic structure and the momentum dependence of the -wave
superconducting gap determined from scanning tunneling microscopy for
CeCoIn, we conclude the robust upward dispersing resonance mode in
CeYbCoIn is inconsistent with the downward dispersion
predicted within the spin-exciton scenario.Comment: Supplementary Information available upon reques
Differentiation of bronchial epithelial spheroids in the presence of IL-13 recapitulates characteristic features of asthmatic airway epithelia
Quadrupolar Susceptibility and Magnetic Phase Diagram of PrNiCd with Non-Kramers Doublet Ground State
In this study, ultrasonic measurements were performed on a single crystal of
cubic PrNiCd, down to a temperature of 0.02 K, to investigate the
crystalline electric field ground state and search for possible phase
transitions at low temperatures. The elastic constant ,
which is related to the -symmetry quadrupolar response, exhibits the
Curie-type softening at temperatures below 30 K, which indicates that the
present system has a non-Kramers doublet ground state. A
leveling-off of the elastic response appears below 0.1 K toward the
lowest temperatures, which implies the presence of level splitting owing to a
long-range order in a finite-volume fraction associated with
-symmetry multipoles. A magnetic field-temperature phase diagram of
the present compound is constructed up to 28 T for [110]. A clear
acoustic de Haas-van Alphen signal and a possible magnetic-field-induced phase
transition at 26 T are also detected by high-magnetic-field
measurements.Comment: 13 pages, 7 figure
Recent advances in the epithelial barrier theory
The epithelial barrier theory links the recent rise in chronic non-communicable diseases, notably autoimmune and allergic disorders, to environmental agents disrupting the epithelial barrier. Global pollution and environmental toxic agent exposure have worsened over six decades because of uncontrolled growth, modernisation, and industrialisation, affecting human health. Introducing new chemicals without any reasonable control of their health effects through these years has led to documented adverse effects, especially on the skin and mucosal epithelial barriers. These substances, such as particulate matter, detergents, surfactants, food emulsifiers, micro- and nano-plastics, diesel exhaust, cigarette smoke and ozone, have been shown to compromise the epithelial barrier integrity. This disruption is linked to the opening of the tight junction barriers, inflammation, cell death, oxidative stress and metabolic regulation. Consideration must be given to the interplay of toxic substances, underlying inflammatory diseases, and medications, especially in affected tissues. This review article discusses the detrimental effect of environmental barrier-damaging compounds on human health and involves cellular and molecular mechanisms
Mechanisms of gut epithelial barrier impairment caused by food emulsifiers polysorbate 20 and polysorbate 80
Background
The rising prevalence of many chronic diseases related to gut barrier dysfunction coincides with the increased global usage of dietary emulsifiers in recent decades. We therefore investigated the effect of the frequently used food emulsifiers on cytotoxicity, barrier function, transcriptome alterations, and protein expression in gastrointestinal epithelial cells.
Methods
Human intestinal organoids originating from induced pluripotent stem cells, colon organoid organ‐on‐a‐chip, and liquid–liquid interface cells were cultured in the presence of two common emulsifiers: polysorbate 20 (P20) and polysorbate 80 (P80). The cytotoxicity, transepithelial electrical resistance (TEER), and paracellular‐flux were measured. Immunofluorescence staining of epithelial tight‐junctions (TJ), RNA‐seq transcriptome, and targeted proteomics were performed.
Results
Cells showed lysis in response to P20 and P80 exposure starting at a 0.1% (v/v) concentration across all models. Epithelial barrier disruption correlated with decreased TEER, increased paracellular‐flux and irregular TJ immunostaining. RNA‐seq and targeted proteomics analyses demonstrated upregulation of cell development, signaling, proliferation, apoptosis, inflammatory response, and response to stress at 0.05%, a concentration lower than direct cell toxicity. A proinflammatory response was characterized by the secretion of several cytokines and chemokines, interaction with their receptors, and PI3K‐Akt and MAPK signaling pathways. CXCL5, CXCL10, and VEGFA were upregulated in response to P20 and CXCL1, CXCL8 (IL‐8), CXCL10, LIF in response to P80.
Conclusions
The present study provides direct evidence on the detrimental effects of food emulsifiers P20 and P80 on intestinal epithelial integrity. The underlying mechanism of epithelial barrier disruption was cell death at concentrations between 1% and 0.1%. Even at concentrations lower than 0.1%, these polysorbates induced a proinflammatory response suggesting a detrimental effect on gastrointestinal health
Gut epithelial barrier damage caused by dishwasher detergents and rinse aids
Background: The increased prevalence of many chronic inflammatory diseases linked to gut epithelial barrier leakiness has prompted us to investigate the role of extensive use of dishwasher detergents, among other factors.
Objective: We sought to investigate the effects of professional and household dishwashers, and rinse agents, on cytotoxicity, barrier function, transcriptome, and protein expression in gastrointestinal epithelial cells.
Methods: Enterocytic liquid-liquid interfaces were established on permeable supports, and direct cellular cytotoxicity, transepithelial electrical resistance, paracellular flux, immunofluorescence staining, RNA-sequencing transcriptome, and targeted proteomics were performed.
Results: The observed detergent toxicity was attributed to exposure to rinse aid in a dose-dependent manner up to 1:20,000 v/v dilution. A disrupted epithelial barrier, particularly by rinse aid, was observed in liquid-liquid interface cultures, organoids, and gut-on-a-chip, demonstrating decreased transepithelial electrical resistance, increased paracellular flux, and irregular and heterogeneous tight junction immunostaining. When individual components of the rinse aid were investigated separately, alcohol ethoxylates elicited a strong toxic and barrier-damaging effect. RNA-sequencing transcriptome and proteomics data revealed upregulation in cell death, signaling and communication, development, metabolism, proliferation, and immune and inflammatory responses of epithelial cells. Interestingly, detergent residue from professional dishwashers demonstrated the remnant of a significant amount of cytotoxic and epithelial barrier-damaging rinse aid remaining on washed and ready-to-use dishware.
Conclusions: The expression of genes involved in cell survival, epithelial barrier, cytokine signaling, and metabolism was altered by rinse aid in concentrations used in professional dishwashers. The alcohol ethoxylates present in the rinse aid were identified as the culprit component causing the epithelial inflammation and barrier damage.
Keywords: Alcohol ethoxylates; Caco-2; cytotoxicity; dishwasher detergents; epithelial barrier; inflammation; rinse aid
DRD4 genotyping may differentiate symptoms of attention-deficit/hyperactivity disorder and sluggish cognitive tempo
Objective: Studies to reduce the heterogeneity of attention-deficit/hyperactivity disorder (ADHD) have increased interest in the concept of sluggish cognitive tempo (SCT). The aim of this study was to investigate if the prevalence of two variable-number tandem repeats (VNTRs) located within the 30 -untranslated region of the DAT1 gene and in exon 3 of the dopamine D4 receptor (DRD4) gene differ among four groups (31 subjects with SCT but no ADHD, 146 individuals with ADHD but no SCT, 67 subjects with SCT + ADHD, and 92 healthy controls). Methods: We compared the sociodemographic profiles, neurocognitive domains, and prevalence of two VNTRs in SCT and ADHD subjects versus typically developing (TD) controls. Results: The SCT without ADHD group had a higher proportion of females and lower parental educational attainment. Subjects in this group performed worse on neuropsychological tests, except for psychomotor speed and commission errors, compared to controls. However, the ADHD without SCT group performed significantly worse on all neuropsychological domains than controls. We found that 4R homozygosity for the DRD4 gene was most prevalent in the ADHD without SCT group. The SCT without ADHD group had the highest 7R allele frequency, differing significantly from the ADHD without SCT group. Conclusion: The 7R allele of DRD4 gene was found to be significantly more prevalent in SCT cases than in ADHD cases. No substantial neuropsychological differences were found between SCT and ADHD subjects
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