Smrzavanje i otapanje uzoraka mlijeka prije nacjepljivanja na hranjivu podlogu poboljšava dijagnosticiranje stafilokoknog mastitisa u krava.

Diagnosis of staphylococcal mastitis cases may sometimes prove problematic due to the nature of the bacterium. Thus, in the present study, the effect of freezing and thawing bovine milk samples on the performance of standard cultures of staphylococcal mastitis was investigated to improve the diagnosis. Each of a total of 228 quarter milk samples from clinical and subclinical mastitis cases was plated on 7 % sheep blood agar directly, and after first being subjected to a process of freezing and thawing. The culture results from two methods were compared on the basis of Staphylococcus spp. positive udder counts and alteration in colony counts of the strains. In the first method, Staphylococcus spp. were isolated from 91 milk samples. In the second method, Staphylococcus spp. were isolated from an additional 11 milk samples that were negative in the first method. Staphylococcus spp. positive udder counts were found to be significantly different between the two methods. The changes in colony counts of the strains between the two methods were also found to be statistically significant in both clinical and subclinical mastitis cases, with the second method performing better. Consequently, these results indicate that a simple preculture step consisting of freezing and thawing milk samples has advantages for more sensitive diagnosis of staphylococcal mastitis in cattle milk.Zbog osobina uzročnika dijagnostika stafi lokoknog mastitisa ponekad može biti otežana. Radi poboljšanja dijagnostike u ovom je istraživanju istražen učinak smrzavanja i otapanja pretraživanih uzoraka mlijeka prije provođenja standardnog postupka uzgoja uzročnika. Svaki od 228 uzetih uzoraka mlijeka kod kliničkog i supkliničkog mastitisa bio je nacijepljen na krvni agar sa 7 % ovčje krvi izravno te nakon postupka smrzavanja i otapanja. Rezultati uzgoja obaju postupaka bili su uspoređeni na osnovi broja vimena pozitivnih na stafilokoke i broja poraslih bakterijskih kolonija. Kod izravnog nacjepljivanja Staphylococcus spp. bio je izdvojen iz 91 uzorka mlijeka. Nakon smrzavanja i otapanja Staphylococcus spp. bio je izdvojen iz još 11 uzoraka, koji su bili negativni kod izravnog nacjepljivanje bez prethodnoga smrzavanja i otapanja. Broj vimena pozitivnih na Staphylococcus spp. bio je statistički značajno različit između tih dvaju pretraživanih postupaka. Razlike u broju kolonija izolata bile su također statistički značajne između rabljenih postupaka kod dijagnosticiranja kliničkog i supkliničkog mastitisa. Boljim se pokazao postupak prethodnog smrzavanja i otapanja uzoraka mlijeka. Rezultati upućuju na zaključak da smrzavanje i otapanje uzoraka mlijeka prije nacjepljivanja na hranjivu podlogu ima prednosti s obzirom da se taj postupak pokazao osjetljivijim za dokazivanje stafilokoka kod pojave mastitisa u mliječnih krava

Measuring the reproducibility and quality of Hi-C data

BACKGROUND: Hi-C is currently the most widely used assay to investigate the 3D organization of the genome and to study its role in gene regulation, DNA replication, and disease. However, Hi-C experiments are costly to perform and involve multiple complex experimental steps; thus, accurate methods for measuring the quality and reproducibility of Hi-C data are essential to determine whether the output should be used further in a study. RESULTS: Using real and simulated data, we profile the performance of several recently proposed methods for assessing reproducibility of population Hi-C data, including HiCRep, GenomeDISCO, HiC-Spector, and QuASAR-Rep. By explicitly controlling noise and sparsity through simulations, we demonstrate the deficiencies of performing simple correlation analysis on pairs of matrices, and we show that methods developed specifically for Hi-C data produce better measures of reproducibility. We also show how to use established measures, such as the ratio of intra- to interchromosomal interactions, and novel ones, such as QuASAR-QC, to identify low-quality experiments. CONCLUSIONS: In this work, we assess reproducibility and quality measures by varying sequencing depth, resolution and noise levels in Hi-C data from 13 cell lines, with two biological replicates each, as well as 176 simulated matrices. Through this extensive validation and benchmarking of Hi-C data, we describe best practices for reproducibility and quality assessment of Hi-C experiments. We make all software publicly available at http://github.com/kundajelab/3DChromatin_ReplicateQC to facilitate adoption in the community

Cutaneous candidiasis in two guinea pigs

WOS: 000297776300013Two approximately 6-month-old guinea pigs were admitted to the Department of Internal Medicine, Faculty of Veterinary (DIMFV), University of Ankara because of lethargy of several weeks' duration, anorexia, bilateral allopecia, and itching. Candida albicans, was isolated from the skin lesions. Both patients were treated with ketoconazole (10 mg/kg PO every 12 h) and topical clotrimazole. The female guinea pig died during the seventh day of the treatment period

On Detection of Edges and Interest Points for Omnidirectional Images in Spherial Domain

In this study, the principle that any single-viewpoint catadioptric projection is equivalent to a spherical projection followed by a point projection is explained. Applicability of Gaussian smoothing, line detection and interest point detection using this principle and stereographic projection is investigated. Particularly, Scale Invariant Feature Transform (SIFT) is emphasized

The effect of sarcopenia and sarcopenic obesity on survival in gastric cancer

Abstract Background Sarcopenic obesity arises from increased muscle catabolism triggered by inflammation and inactivity. Its significance lies in its role in contributing to morbidity and mortality in gastric cancer. This study aims to explore the potential correlation between sarcopenia, sarcopenic obesity, and gastric cancer, as well as their effect on survival. Materials and methods This retrospective study included 162 patients aged ≥ 18 years who were diagnosed with stomach cancer. Patient age, gender, diagnostic laboratory results, and cancer characteristics were documented. Sarcopenia was assessed using the skeletal muscle index (SMI) (cm2/m2), calculated by measuring muscle mass area from a cross-sectional image at the L3 vertebra level of computed tomography (CT). Results Among the 162 patients, 52.5% exhibited sarcopenia (with cut-off limits of 52.4 cm2/m2 for males and 38.5 cm2/m2 for females), and 4.9% showed sarcopenic obesity. Average skeletal muscle area (SMA) was 146.8 cm2; SMI was 50.6 cm2/m2 in men and 96.9 cm2 and 40.6 cm2/m2 in women, respectively. Sarcopenia significantly reduced mean survival (p = 0.033). There was no association between sarcopenic obesity and mortality (p > 0.05), but mortality was higher in sarcopenic obesity patients (p = 0.041). Patient weight acted as a protective factor against mortality, supporting the obesity paradox. Tumor characteristics, metabolic parameters, and concurrent comorbidities did not significantly impact sarcopenia or mortality. Conclusion Sarcopenia is more prevalent in the elderly population and is linked to increased mortality in gastric cancer patients. Paradoxically, higher body mass index (BMI) was associated with improved survival. Computed tomography offers a practical and reliable method for measuring muscle mass and distinguishing these distinctions. Trial registration This study was approved by Istanbul Training and Research Hospital Clinical Research Ethics Committee of the University of Health Sciences (29.05.2020/2383)

Prevalence, Underlying Factors And Damage Associated With Chronic Persistent Inflammation In Patients With Fmf

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Application Of Auto-Inflammatory Disease Damage Index (Addi) To Patients With Fmf And Related Factors With Damage

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