MicroRNA-146a deficiency delays wound healing in normal and diabetic mice

OBJECTIVE: MiRNAs are important regulators of inflammation and wound healing. However, the mechanisms through which miRNAs regulate wound healing under normal and diabetic conditions are poorly understood. We aimed to determine the effects of miR-146a on the pathogenesis of wound healing in normal and streptozotocin (STZ)-induced diabetic mice. APPROACH: Wild-type (WT) and miR-146a knockout (KO) mice were induced to develop diabetes with STZ. Next, skin and corneal wounds were produced and measured. Percent wound closure and histology were evaluated. Inflammation at wound sites was analyzed using flow cytometry, RT-PCR, and Western blot. RESULTS: Healing of wounded skin was significantly delayed in miR-146a KO compared to WT mice. However, corneal epithelial wound healing did not differ significantly in the mice with normal blood glucose, whereas corneal and skin wound healing was significantly delayed in KO mice with diabetes. Neutrophil infiltration increased in skin wounds of KO compared with normal mice. The potential mechanisms were associated with dysregulated IL-1β, TNF-α, IRAK1, TRAF6, and NF-κB signaling induced by miR-146a KO. INNOVATION: Skin wound healing was delayed in miR-146a KO mice and enhanced inflammatory responses were mediated by the NF-κB signaling pathway. CONCLUSIONS: Deficiency in miR-146a delayed skin wound healing by enhancing inflammatory responses in normal and diabetic mice. Therefore, miR-146a may be a potential target for modulation to accelerate skin wound healing

MOESM1 of Illumina-based transcriptomic profiling of Panax notoginseng in response to arsenic stress

Additional file 1: Figure S1. Gene Ontology classification of matched unigenes. 55,291 GO annotated unigenes were classified into 3 functional categories: biological process, cellular component and molecular function. Figure S2. KOG functional classification of matched unigenes. 32,507 KOG annotated unigenes were clustered into 26 categories. Figure S3. KEGG classification of assembled unigenes. 26,245 unigenes were assigned to 5 KEGG biochemical pathways: metabolism, genetic information processing, organism system, cellular processes and environmental information processing. Figure S4. Enriched GO terms of up-regulated genes. Genes up-regulated with As exposure were subjected to GO analysis. Figure S5. Enriched GO terms of down-regulated genes. Genes down-regulated with As exposure were subjected to GO analysis

Dietary Patterns Associated with Cognitive Function among the Older People in Underdeveloped Regions: Finding from the NCDFaC Study

Although dietary patterns are crucial to cognitive function, associations of dietary patterns with cognitive function have not yet been fully understood. This cross-sectional study explored dietary patterns associated with cognitive function among the older adults in underdeveloped regions, using 1504 community-dwelling older adults aged 60 and over. Diet was assessed using a food frequency questionnaire and 24-h dietary recall. Factor analysis was used to extract dietary patterns. Global cognitive function was assessed using the Mini-Mental State Examination (MMSE). Two dietary patterns, a “mushroom, vegetable, and fruits” (MVF) pattern and a “meat and soybean products” (MS) pattern, were identified. The MVF pattern, characterized by high consumption of mushrooms, vegetables, and fruits was significantly positively associated with cognitive function (p < 0.05), with an odds ratio of (95% CIs) 0.60 (0.38, 0.94) for cognitive impairment and β (95% CIs) 0.15 (0.02, 0.29) for –log (31-MMSE score). The MS pattern, characterized by high consumption of soybean products and meat, was also associated with better cognitive function, with an odds ratio of 0.47 (95% CIs 0.30, 0.74) for cognitive impairment and β (95% CIs) 0.34 (0.21, 0.47) for –log (31-MMSE score). Our results suggested that both the MVF and MS patterns were positively associated with better cognitive function among older adults in underdeveloped regions