A note on the adjacent vertex distinguishing total chromatic number of graphs

AbstractAn adjacent vertex distinguishing total coloring of a graph G is a proper total coloring of G such that any pair of adjacent vertices have different sets of colors. The minimum number of colors needed for such a total coloring of G is denoted by χa″(G). In this note, we show that χa″(G)≤2Δ for any graph G with maximum degree Δ≥3

Genetically engineered magnetic nanocages for cancer magneto-catalytic theranostics

磁热疗法是一种利用磁热敏剂在磁场中把磁能转换为热能以杀死肿瘤的新型癌症治疗方法，并已成功应用于临床。但是，目前临床所用磁热敏剂的磁-热转换效率低而使得治疗剂量过大，从而给病人带来潜在的副作用，因此大大限制了磁热疗法的广泛应用。该研究利用基因工程和仿生矿化技术制备出具有优异磁-热转化能力及纳米酶催化性能的磁性蛋白纳米笼（eMIONs），成功克服了临床磁热疗法中磁热敏剂低效的瓶颈，为新一代磁热敏剂的研发提供新的思路。该研究工作在刘刚教授指导下完成，博士生张阳为文章第一作者。【Abstract】The clinical applications of magnetic hyperthermia therapy (MHT) have been largely hindered by the poor magnetic-to-thermal conversion efficiency of MHT agents. Herein, we develop a facile and efficient strategy for engineering encapsulin-produced magnetic iron oxide nanocomposites (eMIONs) via a green biomineralization procedure. We demonstrate that eMIONs have excellent magnetic saturation and remnant magnetization properties, featuring superior magnetic-to-thermal conversion efficiency with an ultrahigh specific absorption rate of 2390 W/g to overcome the critical issues of MHT. We also show that eMIONs act as a nanozyme and have enhanced catalase-like activity in the presence of an alternative magnetic field, leading to tumor angiogenesis inhibition with a corresponding sharp decrease in the expression of HIF-1α. The inherent excellent magnetic-heat capability, coupled with catalysis-triggered tumor suppression, allows eMIONs to provide an MRI-guided magneto-catalytic combination therapy, which may open up a new avenue for bench-to-bed translational research of MHT.This work was supported by the Major State Basic Research Development Program of China (2017YFA0205201), the National Natural Science Foundation of China (81925019, 81422023, 81603015, 81871404, and U1705281), the Fundamental Research Funds for the Central Universities (20720190088 and 20720200019), and the Program for New Century Excellent Talents in University, China (NCET-13-0502). We acknowledge Jingru Huang and Baoying Xie from Central Laboratory in School of Medicine, Xiamen University, for assistance with inductively coupled plasma experiments and data analysis. 研究工作得到了科技部重大专项课题、973课题、国家自然科学基金委杰出青年基金等项目的支持

PwHAP5, a CCAAT-binding transcription factor, interacts with PwFKBP12 and plays a role in pollen tube growth orientation in Picea wilsonii

The HAP complex occurs in many eukaryotic organisms and is involved in multiple physiological processes. Here it was found that in Picea wilsonii, HAP5 (PwHAP5), a putative CCAAT-binding transcription factor gene, is involved in pollen tube development and control of tube orientation. Quantitative real-time reverse transcription-PCR showed that PwHAP5 transcripts were expressed strongly in germinating pollen and could be induced by Ca2+. Overexpression of PwHAP5 in pollen altered pollen tube orientation, whereas the tube with PwHAP5RNAi showed normal growth without diminishing pollen tube growth. Furthermore, PwFKBP12, which encodes an FK506-binding protein (FKBP) was screened and a bimolecular fluorescence complementation assay performed to confirm the interaction of PwHAP5 and PwFKBP12 in vivo. Transient expression of PwFKBP12 in pollen showed normal pollen tube growth, whereas the tube with PwFKBP12RNAi bent. The phenotype of overexpression of HAP5 on pollen tube was restored by FKBP12. Altogether, our study supported the role of HAP5 in pollen tube development and orientation regulation and identified FKBP12 as a novel partner to interact with HAP5 involved in the process

Searching and Tracking an Unknown Number of Targets: A Learning-Based Method Enhanced with Maps Merging

Unmanned aerial vehicles (UAVs) have been widely used in search and rescue (SAR) missions due to their high flexibility. A key problem in SAR missions is to search and track moving targets in an area of interest. In this paper, we focus on the problem of Cooperative Multi-UAV Observation of Multiple Moving Targets (CMUOMMT). In contrast to the existing literature, we not only optimize the average observation rate of the discovered targets, but we also emphasize the fairness of the observation of the discovered targets and the continuous exploration of the undiscovered targets, under the assumption that the total number of targets is unknown. To achieve this objective, a deep reinforcement learning (DRL)-based method is proposed under the Partially Observable Markov Decision Process (POMDP) framework, where each UAV maintains four observation history maps, and maps from different UAVs within a communication range can be merged to enhance UAVs’ awareness of the environment. A deep convolutional neural network (CNN) is used to process the merged maps and generate the control commands to UAVs. The simulation results show that our policy can enable UAVs to balance between giving the discovered targets a fair observation and exploring the search region compared with other methods

Evaluation and Design of Colored Silicon Nanoparticle Systems Using a Bidirectional Deep Neural Network

Silicon nanoparticles (SiNPs) with lowest-order Mie resonance produce non-iridescent and non-fading vivid structural colors in the visible range. However, the strong wavelength dependence of the radiation pattern and dielectric function makes it very difficult to design nanoparticle systems with the desired colors. Most existing studies focus on monodisperse nanoparticle systems, which are unsuitable for practical applications. This study combined the Lorentz–Mie theory, Monte Carlo, and deep neural networks to evaluate and design colored SiNP systems. The effects of the host medium and particle size distribution on the optical and color properties of the SiNP systems were investigated. A bidirectional deep neural network achieved accurate prediction and inverse design of structural colors. The results demonstrated that the particle size distribution flattened the Mie resonance peak and influenced the reflectance and brightness of the SiNP system. The SiNPs generated vivid colors in all three of the host media. Meanwhile, our proposed neural network model achieved a near-perfect prediction of colors with high accuracy of the designed geometric parameters. This work accurately and efficiently evaluates and designs the optical and color properties of SiNP systems, thus accelerating the design process and contributing to the practical production design of color inks, decoration, and printing

Modification of Human Umbilical Cord Blood Stem Cells Using Polyethylenimine Combined with Modified TAT Peptide to Enhance BMP-2 Production

With the emerging role of umbilical cord blood-derived mesenchymal stem cells (hUCB-MSC) for bone regeneration and delivery of therapeutic proteins, there is an increasing need for effective gene delivery systems to modify such cells. mTAT, a TAT peptide sequence bearing histidine and cysteine residues, has been successfully used for intracellular gene delivery. Using a gWiz-GFP plasmid, we demonstrated that polyethylenimine combined with mTAT (mTAT/PEI) displayed good transfection efficacy in hUCB-MSC. hUCB-MSC transfected with mTAT/PEI were shown to express more BMP-2 protein and mRNA, indicating the feasibility of using the cells as a BMP-2 delivery system. Importantly, compared to PEI25, a “gold standard” nonviral transfection polymer, mTAT/PEI had limited toxicity to the cells. Furthermore, we demonstrated enhanced osteogenic activity in vitro for BMP-2 expressing hUCB-MSC. These results provide encouraging evidence for the potential use of mTAT/PEI to genetically modify hUCB-MSC as an approach to enhance tissue regeneration

Prevalence and Risk Factors for Pterygium in Rural Older Adults in Shandong Province of China: A Cross-Sectional Study

To investigate the prevalence and risk factors for pterygium in rural older adults in Shandong Province, eastern China, a population-based, cross-sectional study was performed from April to July 2008. By means of cluster random sampling methods, a total of 19,583 people aged 50 years or above were randomly selected from four rural counties. Out of 19,583 people, 1,767 residents were excluded mainly because they were migrant workers when this study was performed. Finally, 17,816 (90.98%) people were included as eligible subjects. They received a comprehensive eye examination and a structured questionnaire voluntarily. Patients with pterygium were defined as having pterygium at the time of survey or pterygium surgery had been performed. 1,876 people were diagnosed as pterygium, either unilateral (1,083) or bilateral (793), which is equivalent to a prevalence of 10.53% (95% CI, 10.08–10.98). The multivariate logistic regression analysis showed that pterygium was independently associated with older age, areas, outdoor time, educational level, and use of hat and/or sunglasses. The prevalence of pterygium increased with age and hours spent under sunshine per day. Meanwhile, the higher the educational level and the more use of hat and/or sunglasses, the lower the pterygium prevalence

miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

Osteoporosis (OP) is a common bone metabolic disease, the process of which is fundamentally irreversible. Therefore, the investigation into osteoblastic differentiation of bone marrow mesenchymal stem cells (BMSCs) will provide more clues for OP treatment. In the present study, we found that microRNA-187-5p (miR-187-5p) played a key role on osteoblastic differentiation, which was significantly upregulated during osteogenic differentiation of BMSCs in mice. Moreover, overexpression of miR-187-5p suppressed osteoblastic differentiation of BMSCs through increasing alkaline phosphatase (ALP), matrix mineralization, and levels of Osterix (OSX), and osteopontin (OPN) as well as runt-related transcription factor 2 (Runx2) in vitro. The results in vivo indicated that the upregulation of miR-187-5p enhanced the efficacy of new bone formation in the heterotopic bone formation assay. Luciferase reporter assay and western blot analysis revealed that miR-187-5p was involved in osteogenesis by targeting intracellular adhesion molecule 1 (ICAM-1). Furthermore, ICAM-1 silence inhibited osteoblastic differentiation of BMSCs. Taken together, our results suggested for the first time that miR-187-5p may promote osteogenesis by targeting ICAM-1, and provided a possible therapeutic target for bone metabolic diseases