Uber die Welch-Fraenkelschen Bazillen und ihre Verwandten im Darmkanal

Der Welch-Fraenkelsche Bazillus, der sich als ein Bodenbakterium weit im Boden verbreitet, ist obligater Anaerobier, und bekannt als Erreger des Gasoedems, welches eine chirurgisch und insbesonders kriegschirurgisch wichtige Wundinfektionskrankheit ist. Die Tatsache, dass dieses Stabchen nicht nur aus dem Boden, sondern auch aus menschlichem Darm gezuchtet wird, hat viele Autoren schon bisweilen beschaftigt. Dieser Bazillus ist Gram-positiv, gross und kraftig, abgerundet, unbeweglich und sporuliert nicht im gewohnlichen Nahrboden. Die Milch wurde sturmisch mit Gasbildung vergoren. Schon nach 20 Stunden schwamm das Kasein deutlich geschieden in truber, immer klarer werdender Molke. Die Kaseingerinnsel wurden ferner nicht wieder durch Peptonisierung gelost, noch kam es nach dem leicht sauerlich bleibenden Geruch zu Eiweissfaulniss. Die oben beschriebene Eigenschaft, die sog. Sturmische Gerinnung , ist charakteristisches Merkmal zur Diagnose-stellung des Welch-Fraenkelschen Bazillus. Der Verfasser hat aus 172 Faeces der Menschen 244 Stamme der Welch-Fraenkelschen Bazillen und 39 ahnliche Stamme, die von erstem Bazillus deutlich voneinander in Eigenstumlichkeit abweicht gezuchtet; d. h. dem Letzteren fehlt die sturmische Gerinnung. Diesen Bazillus nennt der Verfasser einen Verwandten der Welch-Fraenkelschen Bazillen , und vergleicht dieses Stabchen morphologisch und biologisch mit den Welch-Fraenkelschen Bazillen, welche er aus menschlichen Faeces und klassischem Gasoedem isoliert hat. Morphologie: Die Verwandten sind ebenso mit abgerundetem Ende 0.8-1.0μ breit, 4.0-1.0μ, nicht haufig 15μ in der Lange, zuweilen leicht gekrummt, und werden zarter als Welch-Fraenkelche Bazillen beobachtet. Sporen und Kapseln: Nicht nur in gewohnlichen Nahrboden, sondern auch in den alkalischen-, natriumphosphathaltigen Nahrsubstraten und im Hirnbrei nahm der Verfasser keine Sporen oder Kapseln wahr. Aber nur bei aus Gasoedem gezuchtetem Stamm beobachtete er beide im spezifischen antiserumhaltigen Nahrboden. Kolonientypen: Auf d

Preparation and Characterization of Biodegradable Composited Films Based on Potato Starch/Glycerol/Gelatin

The use of plastics is resisted worldwide. Therefore, finding alternatives to plastic packaging products is an urgent issue. This work was dedicated to the preparation of biodegradable composited films with potato starch, glycerol, and gelatin. The formulation of the biodegradable film was first optimized via response surface methodology combined with the multi-index comprehensive evaluation method that considered physical properties (thickness, water solution (WS), tensile strength (TS) and elongation at break (E%)) and barrier property (light transmittance (T%)). Results indicated that the optimal conditions were 2.5% starch, 2.0% glycerol, and 1.5% gelatin (based on water). The optimized film presented excellent properties with TS of 4.47 MPa, E% of 109.91%, WS of 43.64%, and T% of 41.21% at 500 nm, and the comprehensive evaluation score of the composite film was 28.68. Moreover, a model verification experiment was further conducted, which proved that the predicted value highly matched experimental values, indicting the credibility and accuracy of the model. The resulting films were further characterized on the basis of rheological measurements, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The rheological measurements proved that the film-forming solution exhibited low shear viscosity and non-Newtonian fluid behavior. FTIR and SEM revealed excellent compatibility among starch, glycerol, and gelatin. Hence, the resulting optimized film may be expected to provide theoretical basis and technical support for the food packing industry

Improvements for Tissue-Chopping-Based Immunofluorescence Staining Method of Chloroplast Proteins

Immunofluorescence staining is a very common method for the subcellular localization study of proteins. A tissue-chopping-based immunofluorescence staining method for chloroplast proteins overcomes the restriction of plant cell wall, makes the operation simpler, and uses less experimental materials. Here we provide some improvements for this method. We found that the stained tissues can be directly observed with a confocal microscope without tissue lysis. Samples maintained at a low temperature (0–4 °C) throughout the process can reduce the intensity of chlorophyll autofluorescence and the background signal. A low temperature is also good for the storage of the sample. Fluorescence signal of the stained samples can be kept for several weeks if they are stored at −20 °C. FtsZ is an essential component of the chloroplast division apparatus. We demonstrated this method with the immunofluorescence staining of FtsZ1 in wildtype Arabidopsis and some chloroplast division mutants. We also successfully tested this method by the immunofluorescence staining of FtsZ1 in many other plants, including woody plants. With these procedures, the performance of tissue-chopping-based immunofluorescence staining method are further improved

Effect of Dicarboxylate Ligands on the Construction of Cd(II) Complexes Based on a Flexible Bis-Triazole Ligand

<div><p>In this work, two dicarboxylate ligands, 4,4′-oxybis(benzoic acid) (H₂oba) and 4,4′-biphenyldicarboxylic acid (H₂bpdc) were employed as coligands to perform a comparison on the structures of Cd(II)-btmx complexes (btmx = 1,4-bis(1,2,4-triazol-1-ylmethyl)-2,3,5,6-tetramethylbenzene) motifs. Two new complexes, {[Cd(btmx)(oba)]·H₂O}_n (1) and [Cd(btmx)(bpdc)]_n (2), have been synthesized and characterized. Structural analyses show that 1 shows a 1D double-chain, while 2 displays an undulating 2D 4⁴-<i>sql</i> network with 2-fold interpenetration. The structural differences of the two complexes suggest that the dicarboxylate ligands have great impact on the formation of such coordination architectures. Furthermore, the photoluminescence properties of 1 and 2 have also been investigated.</p></div

Combining gray matter volume in the cuneus and the cuneus-prefrontal connectivity may predict early relapse in abstinent alcohol-dependent patients

<div><p>Background</p><p>Developing more effective strategies to prevent relapse remains one of the major challenges of treating substance dependence. Previous studies have identified brain abnormalities in abstinent alcoholics. However, whether these persistent brain deficits in abstinence could predict early relapse to alcohol use has not been well established. This study aimed to identify biomarkers of relapse vulnerability by investigating persistent brain abnormalities in abstinent alcohol-dependent patients.</p><p>Methods</p><p>Brain imaging and impulsive behavior data were collected from 56 abstinent alcohol-dependent male inpatients and 33 age-matched male healthy controls. Voxel-based morphometry was used to investigate the differences of grey matter volume between the groups. The resting-state functional connectivity was examined using brain areas with gray matter deficits as seed regions. A preliminary prospective study design was used to classify patients into abstainers and relapsers after a 62-day average abstinence period.</p><p>Results</p><p>Compared with healthy controls, both relapsers and abstainers exhibited significantly reduced gray matter volume in the cuneus. Functional connectivity analysis revealed that relapsers relative to abstainers demonstrated increased cuneus-centered negative functional connectivity within a network of brain regions which are involved in executive control and salience. Abnormal gray matter volume in the left cuneus and the functional connectivity between the right cuneus and bilateral dorsolateral prefrontal cortex could successfully predict relapse during the 3-month follow-up period.</p><p>Conclusions</p><p>Findings suggest that the abnormal gray matter volume in the cuneus and resting-state cuneus-prefrontal functional connectivity may play an important role in poor treatment outcomes in alcoholics and serve as useful neural markers of relapse vulnerability.</p></div