23 research outputs found
Targeted Gene Delivery Therapies for Cervical Cancer
Despite being largely preventable through early vaccination and screening strategies,
cervical cancer is the most common type of gynecological malignancy worldwide and constitutes one
of the leading causes of cancer deaths in women. Patients with advanced or recurrent disease have a
very poor prognosis; hence, novel therapeutic modalities to improve clinical outcomes in cervical
malignancy are needed. In this regard, targeted gene delivery therapy is presented as a promising
approach, which leads to the development of multiple strategies focused on different aspects. These
range from altered gene restoration, immune system potentiation, and oncolytic virotherapy to the
use of nanotechnology and the design of improved and enhanced gene delivery systems, among
others. In the present manuscript, we review the current progress made in targeted gene delivery
therapy for cervical cancer, the advantages and drawbacks and their clinical application. At present,
multiple targeted gene delivery systems have been reported with encouraging preclinical results.
However, the translation to humans has not yet shown a significant clinical benefit due principally to
the lack of efficient vectors. Real efforts are being made to develop new gene delivery systems, to
improve tumor targeting and to minimize toxicity in normal tissues.This research was supported by the Fundación Mutua Madrileña (project FMM-AP16683-2017) and
Consejería de Salud Junta de Andalucía (PI-0089-2017)
Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture
There is a global need to discover effective anti-cancerous compounds from natural sources.
Cultivated wheat cells can be a valuable source of non-toxic or low toxic plant-derived polysaccharides.
In this study, we evaluated the anti-cancer ability of seven fractions of wheat cell culture
polysaccharides (WCCPSs) in the HCT-116 colon cancer cell line. Almost all (6/7) fractions had
an inhibitory effect on the proliferation of colon cancer cells, and two fractions (A-b and A-f) had
considerable therapeutic indexes. The WCCPS fractions induced cell cycle arrest in the G1 phase
and induced different rates of apoptosis ( 48%). Transmission and scanning electron microscopy
revealed that WCCPS fractions caused apoptotic changes in the nucleus and cytoplasm, including
damage to mitochondria and external morphological signs of apoptosis. In addition, the WCCPSs
induced an increase in the levels of Bax, cytochrome c, and caspases 8 and 3, indicating that cell
death progressed through intrinsic and extrinsic pathways of apoptosis. Furthermore, some fractions
caused a significant decrease of c-Myc, b-catenin, NFkB2, and HCAM (CD 44) levels, indicating
enhanced cell differentiation. Thus, for the first time, our results provide a proof of concept of the
anti-cancer capacity of WCCPS fractions in colorectal cancer.FEDER Operational Program 2020/Junta de AndaluciaConsejeria de Economia y Conocimiento/Project B-CTS-562-UGR20Chair "Doctors GaleraRequena in cancer stem cell research" CMC-CTS963Erasmus+ Mobility ProgramRepublic of KazakhstanScottish Government's Rural and Environment Science and Analytical Services (RESAS) divisio
Silver Nanoparticles from Annona muricata Peel and Leaf Extracts as a Potential Potent, Biocompatible and Low Cost Antitumor Tool
Cancer is one of the most prevalent diseases in the world and requires new therapies for
its treatment. In this context, the biosynthesis of silver nanoparticles (AgNPs) has been developed
to treat different types of tumors. The Annona muricata plant is known for having anticancer activity.
Its main compounds present in the leaves, stems and skin, allowing for its use as reducing agents.
In this manuscript, AgNPs with leaf extract (AgNPs-LE) and fruit peel extract (AgNPs-PE) of A.
muricata were biosynthesized obtaining an average nanoparticle diameter sizes smaller than 50 nm,
being 19.63 ± 3.7 nm and 16.56 ± 4.1 nm, and with a surface plasmonic resonance (SPR) at 447 and
448 nm, respectively. The lactone functional group present in the LE and PE extracts was identified
by the FTIR technique. The behavior and antiproliferation activity of AgNPs-LE and AgNPs-PE
were evaluated in breast, colon and melanoma cancer cell lines. Our results showed that Annona
muricata fruit peel, which is a waste product, has an antitumor effect more potent than leaf extract.
This difference is maintained with AgNPs where the destruction of cancer cells was, for the first
time, achieved using concentrations that do not exceed 3 μg/mL with a better therapeutic index in
the different tumor strains. In conclusion, we present a low-cost one-step experimental setup to
generate AgNPs-PE whose in-vitro biocompatibility and powerful therapeutic effect make it a very
attractive tool worth exploiting.Fundacion Empresa Universidad de Granada (Project
PR/18/001)Fundación Mutua Madrileña (Project FMM-AP16683-2017)Consejería de Salud Junta
de Andalucía (PI-0089-2017)Instituto de Salud Carlos III (RTI2018-101309-B-C22
Recent Progress in Gene Therapy for Ovarian Cancer
Ovarian cancer is the most lethal gynecological malignancy in developed countries. This is
due to the lack of specific symptoms that hinder early diagnosis and to the high relapse rate after
treatment with radical surgery and chemotherapy. Hence, novel therapeutic modalities to improve
clinical outcomes in ovarian malignancy are needed. Progress in gene therapy has allowed the
development of several strategies against ovarian cancer. Most are focused on the design of improved
vectors to enhance gene delivery on the one hand, and, on the other hand, on the development
of new therapeutic tools based on the restoration or destruction of a deregulated gene, the use of
suicide genes, genetic immunopotentiation, the inhibition of tumour angiogenesis, the alteration
of pharmacological resistance, and oncolytic virotherapy. In the present manuscript, we review
the recent advances made in gene therapy for ovarian cancer, highlighting the latest clinical trials
experience, the current challenges and future perspectives.This research was supported by the Fundación Mutua Madrileña by the proyect FMM-AP16683-2017,
Consejería de Salud Junta de Andalucía (PI-0089-2017) and from the Chair “Doctors Galera-Requena in cancer
stem cell research”
Ldrb toxin with in vitro and in vivo antitumor activity as a potential tool for cancer gene therapy
Due to the high prevalence of cancer in recent years, it is necessary to develop new and more effective therapies that produce fewer side effects. Development of gene therapy for cancer based on the use of suicide genes that can damage the tumor cell, without requiring a prodrug for its lethal effect, is one of the recent foci of gene therapy strategies. We evaluated the cytotoxic impact of the LdrB toxin from Escherichia coli k12 as a possible tool for cancer gene therapy. For that, colorectal and breast cancer cells were transfected under the control of a TRE3G promoter inducible by doxycycline. Our results showed that ldrB gene expression induced a drastic inhibition of proliferation in vitro, in both 2D and 3D experimental models. Moreover, unlike conventional chemotherapy, the ldrB gene induced a severe loss of proliferation in vivo without any side effects in our animal model. This antitumor outcome was modulated by cell cycle arrest in the G0/G1 phase and apoptotic death. Scanning electronic microscopy demonstrates that the LdrB toxin conserves its pore-forming ability in HCT-116 cells as in E. coli k12. Taken together, our results provide, for the first time, a proof of concept of the antitumor capacity of the ldrB gene in colorectal and breast cancer.This research was supported by the Fundación Mutua Madrileña (project FMM-AP16683-2017),
Consejería de Salud Junta de Andalucía (PI-0089-2017), the MNat Scientitc Unit of Excellence (UCE.PP2017.0f),
and from the Chair “Doctors Galera-Requena in cancer stem cell research”
Nutrición parenteral domiciliaria en España, 2019: informe del Grupo de Nutrición Artificial Domiciliaria y Ambulatoria NADYA
RESUMEN Objetivo: comunicar los datos de nutrición parenteral domiciliaria (NPD) obtenidos del registro del grupo NADYA-SENPE (www.nadyasenpe.com) del año 2019. Material y métodos: análisis descriptivo de los datos recogidos de pacientes adultos y pediátricos con NPD en el registro NADYA-SENPE desde el 1 de enero al 31 de diciembre de 2019. Resultados: se registraron 283 pacientes (51,9 %, mujeres), 31 niños y 252 adultos procedentes de 47 hospitales españoles, lo que representa una tasa de prevalencia de 6,01 pacientes/millón de habitantes/año 2019. El diagnóstico más frecuente en los adultos fue “oncológico paliativo” y “otros” (21,0 %). En los niños fue la enfermedad de Hirschsprung junto a la enterocolitis necrotizante, las alteraciones de la motilidad intestinal y la pseudoobstrucción intestinal crónica, con 4 casos cada uno (12,9 %). El primer motivo de indicación fue el síndrome del intestino corto tanto en los niños (51,6 %) como en los adultos (37,3 %). El tipo de catéter más utilizado fue el tunelizado tanto en los niños (75,9 %) como en los adultos (40,8 %). Finalizaron 68 episodios, todos en adultos: la causa más frecuente fue el fallecimiento (54,4 %). Pasaron a la vía oral el 38,2 %. Conclusiones: el número de centros y profesionales colaboradores con el registro NADYA va incrementándose. Se mantienen estables las principales indicaciones y los motivos de finalización de la NPD
Jardins per a la salut
Facultat de Farmàcia, Universitat de Barcelona. Ensenyament: Grau de Farmàcia. Assignatura: Botànica farmacèutica. Curs: 2014-2015. Coordinadors: Joan Simon, Cèsar Blanché i Maria Bosch.Els materials que aquí es presenten són el recull de les fitxes botàniques de 128 espècies presents en el Jardí Ferran Soldevila de l’Edifici Històric de la UB. Els treballs han estat realitzats manera individual per part dels estudiants dels grups M-3 i T-1 de l’assignatura Botànica Farmacèutica durant els mesos de febrer a maig del curs 2014-15 com a resultat final del Projecte d’Innovació Docent «Jardins per a la salut: aprenentatge servei a Botànica farmacèutica» (codi 2014PID-UB/054). Tots els treballs s’han dut a terme a través de la plataforma de GoogleDocs i han estat tutoritzats pels professors de l’assignatura. L’objectiu principal de l’activitat ha estat fomentar l’aprenentatge autònom i col·laboratiu en Botànica farmacèutica. També s’ha pretès motivar els estudiants a través del retorn de part del seu esforç a la societat a través d’una experiència d’Aprenentatge-Servei, deixant disponible finalment el treball dels estudiants per a poder ser consultable a través d’una Web pública amb la possibilitat de poder-ho fer in-situ en el propi jardí mitjançant codis QR amb un smartphone
III Jornadas de Jóvenes Investigadores en Arqueología: Libro de Actas
Libro de Actas de las III Jornadas de Jóvenes Investigadores en Arqueologí
Nutrición parenteral domiciliaria en España 2018. Informe del Grupo de Nutrición Artificial Domiciliaria y Ambulatoria NADYA
Aim: To communicate home parenteral nutrition (HPN) data obtained from the HPN registry of the NADYA-SENPE group (www.nadya-senpe. com) for the year 2018. Material and methods: Descriptive analysis of the data collected from adult and pediatric patients with HPN in the NADYA-SENPE group registry from January 1st, 2018 to December 31st, 2018. Results: There were 278 patients from 45 Spanish hospitals (54.7% women), 23 children and 255 adults, which represent a prevalence rate of 5.95 patients/million inhabitants/year 2018. The most frequent diagnosis in adults was “palliative cancer” (22.0%), followed by “others”. In children it was Hirschsprung’s disease together with necrotizing enterocolitis, with four cases (17.4%). The first indication was short bowel syndrome in both children (60.9%) and adults (35.7%). The most frequently used type of catheter was tunneled in both children (81.0%) and adults (41.1%). Ending 75 episodes, the most frequent cause was death (52.0%) and change to oral feeding (33.3%). Conclusions: The number of centers and collaborating professionals in the registry of patients receiving HPN remains stable, as well as the main indications and reasons for termination of HPN.Objetivo: comunicar los datos de nutrición parenteral domiciliaria
(NPD) obtenidos del registro del grupo NADYA-SENPE (www.nadyasenpe.
com) del año 2018.
Material y métodos: análisis descriptivo de los datos recogidos de
pacientes adultos y pediátricos con NPD en el registro NADYA-SENPE
del 1 de enero al 31 de diciembre de 2018.
Resultados: se registraron 278 pacientes (54,7% mujeres), 23 niños
y 255 adultos, procedentes de 45 hospitales españoles, lo que
representa una tasa de prevalencia de 5,95 pacientes/millón de
habitantes/año 2018. El diagnóstico más frecuente en adultos fue
“oncológico paliativo” (22,0%), seguido de “otros”. En niños fue la
enfermedad de Hirschsprung junto con la enterocolitis necrotizante,
con cuatro casos (17,4%). El primer motivo de indicación fue
síndrome de intestino corto tanto en niños (60,9%) como en adultos
(35,7%). El tipo de catéter más utilizado fue el tunelizado tanto en
niños (81,0%) como en adultos (41,1%). Finalizaron 75 episodios, la
causa más frecuente fue el fallecimiento (52,0%) y el paso a vía oral
(33,3%).
Conclusiones: el número de centros y profesionales colaboradores
en el registro de pacientes que reciben NPD se mantiene estable, así
como las principales indicaciones y los motivos de finalización de la
NPD
Nutrición parenteral domiciliaria en España 2018. Informe del Grupo de Nutrición Artificial Domiciliaria y Ambulatoria NADYA
Aim: to communicate home parenteral nutrition (HPN) data obtained from the HPN registry of the NADYA-SENPE group (www.nadya-senpe. com) for the year 2018. Material and methods: descriptive analysis of the data collected from adult and pediatric patients with HPN in the NADYA-SENPE group registry from January 1st, 2018 to December 31st, 2018. Results: there were 278 patients from 45 Spanish hospitals (54.7 % women), 23 children and 255 adults, which represent a prevalence rate of 5.95 patients/million inhabitants/year 2018. The most frequent diagnosis in adults was " palliative cancer" (22.0 %), followed by "others". In children it was Hirschsprung's disease together with necrotizing enterocolitis, with four cases (17.4 %). The first indication was short bowel syndrome in both children (60.9 %) and adults (35.7 %). The most frequently used type of catheter was tunneled in both children (81.0 %) and adults (41.1 %). Ending 75 episodes, the most frequent cause was death (52.0 %) and change to oral feeding (33.3 %). Conclusions: the number of centers and collaborating professionals in the registry of patients receiving HPN remains stable, as well as the main indications and reasons for termination of HPN