Cancer is one of the most prevalent diseases in the world and requires new therapies for
its treatment. In this context, the biosynthesis of silver nanoparticles (AgNPs) has been developed
to treat different types of tumors. The Annona muricata plant is known for having anticancer activity.
Its main compounds present in the leaves, stems and skin, allowing for its use as reducing agents.
In this manuscript, AgNPs with leaf extract (AgNPs-LE) and fruit peel extract (AgNPs-PE) of A.
muricata were biosynthesized obtaining an average nanoparticle diameter sizes smaller than 50 nm,
being 19.63 ± 3.7 nm and 16.56 ± 4.1 nm, and with a surface plasmonic resonance (SPR) at 447 and
448 nm, respectively. The lactone functional group present in the LE and PE extracts was identified
by the FTIR technique. The behavior and antiproliferation activity of AgNPs-LE and AgNPs-PE
were evaluated in breast, colon and melanoma cancer cell lines. Our results showed that Annona
muricata fruit peel, which is a waste product, has an antitumor effect more potent than leaf extract.
This difference is maintained with AgNPs where the destruction of cancer cells was, for the first
time, achieved using concentrations that do not exceed 3 μg/mL with a better therapeutic index in
the different tumor strains. In conclusion, we present a low-cost one-step experimental setup to
generate AgNPs-PE whose in-vitro biocompatibility and powerful therapeutic effect make it a very
attractive tool worth exploiting.Fundacion Empresa Universidad de Granada (Project
PR/18/001)Fundación Mutua Madrileña (Project FMM-AP16683-2017)Consejería de Salud Junta
de Andalucía (PI-0089-2017)Instituto de Salud Carlos III (RTI2018-101309-B-C22
