15 research outputs found
LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues
<p>Abstract</p> <p>Background</p> <p>Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues or fungal mycelium.</p> <p>Findings</p> <p>We report on the development of a rapid and relatively inexpensive method for total RNA extraction from plants and fungus-infected tissues, as well as from insects and fungi, based on guanidine hydrochloride buffer and common DNA extraction columns originally used for the extraction and purification of plasmids and cosmids.</p> <p>Conclusions</p> <p>The proposed method can be used reproducibly for RNA isolation from a variety of plant species. It can also be used with infected plant tissue and fungal mycelia, which are typically recalcitrant to standard nucleic acid extraction procedures.</p
Solving loop equations by Hitchin systems via holography in large-N QCD_4
For (planar) closed self-avoiding loops we construct a "holographic" map from
the loop equations of large-N QCD_4 to an effective action defined over
infinite rank Hitchin bundles. The effective action is constructed densely
embedding Hitchin systems into the functional integral of a partially quenched
or twisted Eguchi-Kawai model, by means of the resolution of identity into the
gauge orbits of the microcanonical ensemble and by changing variables from the
moduli fields of Hitchin systems to the moduli of the corresponding holomorphic
de Rham local systems. The key point is that the contour integral that occurs
in the loop equations for the de Rham local systems can be reduced to the
computation of a residue in a certain regularization. The outcome is that, for
self-avoiding loops, the original loop equations are implied by the critical
equation of an effective action computed in terms of the localisation
determinant and of the Jacobian of the change of variables to the de Rham local
systems. We check, at lowest order in powers of the moduli fields, that the
localisation determinant reproduces exactly the first coefficient of the beta
function.Comment: 65 pages, late