Esthetic Management of Midline Diastema by Combined Surgical and Prosthetic Approach

Maxillary midline diastemas are a common esthetic problem that dentists must treat. Many innovative therapies have been used, varying from restorative procedures to surgery (frenectomies) and orthodontics. The need for treatment is mainly attributed to esthetic and psychological reasons, rather than functional ones. Present case report is about 34 year old female patient whose midline diastema was managed by surgical and prosthetic approach

Inhibition of Mycobacterium tuberculosis MtrA response regulator by anticancer drugs via computational methods

Mycobacterium tuberculosis (MTB) causes TB disease and millions of deaths are reported every year. Drug resistance TB and its complex treatment is a big problem worldwide. The present  study aimed to design new and safer antitubercular compounds to tackle this serious threat. The unique drug target is the MtrAB Two-component regulatory system (2CRS) of mycobacteria. MtrAB system consists of MtrB sensor kinase (SK) and MtrA response regulator (RR). This system is essential in MTB and is involved in mycobacteria's proliferation. This important physiological process is operated by the phosphorylation of MtrB and then to MtrA. The phosphorylation mechanism triggers modulation in the expression of MtrA targets genes and helps perform appropriate function. This phenomenon depends on the active and inactive confirmation of MtrA, which involves a ligand (Metal ion complex e.g. Mg2+). In this study, anti-cancerous compounds were selected for the inhibition of MtrA. However, molecular docking exhibited binding affinity ranging from −10.8 to −4.7 kcal/mol, targeting the binding pocket of the selected Tuberculosis–MtrA protein (PDB ID: 5L8X). This energy difference between the native ligand and docked compounds showed that the six molecules: (Risperidone, 2-(benzofuran-2-yl)-6,7-dimethyl-4H-chromen-4-one, (2E)-1-(4-hydroxyphenyl)-3-(quinolin-4-yl)prop-2-en-1-one, Estradiol Cypionate, (2Z)-6-hydroxy-2-(3,4,5-trimethoxybenzylidene)-1-benzofuran-3(2H)-one, (2E)-3-(2,3-dihydro-1,4-benzodioxin-6-yl)-1-(3-hydroxyphenyl)prop-2-en-1-one) mentioned are more potent than the native ligand.These six molecules were first time reported as the inhibitor for MtrA of MtrAB Two-component regulatory system and can be utelize for further study

Microfluidic Platforms to Unravel Mysteries of Alzheimer's Disease: How Far Have We Come?

Alzheimer’s disease (AD) is a significant health concern with enormous social and economic impact globally. The gradual deterioration of cognitive functions and irreversible neuronal losses are primary features of the disease. Even after decades of research, most therapeutic options are merely symptomatic, and drugs in clinical practice present numerous side effects. Lack of effective diagnostic techniques prevents the early prognosis of disease, resulting in a gradual deterioration in the quality of life. Furthermore, the mechanism of cognitive impairment and AD pathophysiology is poorly understood. Microfluidics exploits different microscale properties of fluids to mimic environments on microfluidic chip-like devices. These miniature multichambered devices can be used to grow cells and 3D tissues in vitro, analyze cell-to-cell communication, decipher the roles of neural cells such as microglia, and gain insights into AD pathophysiology. This review focuses on the applications and impact of microfluidics on AD research. We discuss the technical challenges and possible solutions provided by this new cutting-edge technique to understand disease-associated pathways and mechanisms

Effect of antioxidants supplementation on the quality of Beetal buck semen stored at 4°C

Aim: An experiment was designed to evaluate the role of Vitamin E and glutathione in improving the seminal parameters during hypothermic storage of liquid semen at 4°C for 72 h. Materials and Methods: Thirty-six semen ejaculates were collected by artificial vagina from 6 bucks (Beetal) during the normal reproduction season (September to November) at weekly interval. The samples were centrifuged, and the seminal plasma was removed. The sperm pellet was diluted with Tris-based extender and divided into three groups. Group T1: Control samples without antioxidants, Group T2: Samples supplemented with tocopherol at 3 mM, and Group T3: Samples supplemented with glutathione at 1 mM. The samples were evaluated for progressive motility, percent liveability, percent abnormal spermatozoa, and acrosome integrity after liquid preservation for 0, 24, 48, and 72 h. The level of lipid peroxidation and antioxidant enzymes, namely, glutathione peroxidase (GPx) and superoxide dismutase (SOD) were estimated after liquid preservation for 0 and 72 h. Results: It was observed that, after storage of semen at 4°C up to 72 h, the progressive sperm motility, percent liveability, percent abnormal spermatozoa, and percent intact acrosomes were significantly (p<0.05) higher in group T2 and T3 samples as compared to control. However, the level of lipid peroxidation in T2 and T3 groups was significantly (p<0.05) lower after 72 h of incubation at 4°C. Similarly, GPx and SOD values were significantly (p<0.05) increased in T2 and T3 groups after 72 h of storage at 4°C. Conclusion: Thus, it can be concluded that Vitamin E and glutathione supplementation at 3 mM and 1 mM, respectively, while preserving the semen samples at 4°C helped in maintaining the seminal parameters up to 72 h and protected the spermatozoa from oxidative damage

Sustainable Management of National Parks and Protected Areas for Conserving Biodiversity in India

Habitat loss due to human activities and climate change is synergistically posing serious threats to the global biodiversity leading to irreversible extinction of several species. In wake of recent extinction, several forests are declared as protected areas where no more human activities are allowed. However, the scope of these protected areas got broadened from mere conservation to poverty alleviation and sustainable development during the past decades. Though these protected areas seem to be supportive of the biodiversity conservation, several challenges and gaps have emerged that need to be addressed for effective conservation and sustainable management in these protected areas. Therefore, the present chapter aims to address the roles, challenges, and approaches for conservation, and sustainable management in protected areas of India. Based on the published literature, we have found that protected areas proved to be a successful strategy for the conservation of wild animals and plants. However, management of poaching, man-wildlife conflicts, funding, extensive resource use, and tourism is still a challenge for some national parks of the country. Although governmental policies have addressed some of these challenges, only limited success has been achieved so far. Therefore, further studies need to assess the efficiency of protected areas for biodiversity conservation and devise the mechanisms for effective sustainable management of these protected areas

Integral measures and framework for green lean six sigma implementation in manufacturing environment

Modern organizations are in quest of initiatives that will facilitate them to be sustainable as well as provide a competitive advantage to remain a continuous stakeholder in the global market. Green Lean Six Sigma (GLSS) is an inclusive approach that not only improves environmental sustainability but also leads to improvement in the financial stability of the organizations. To implement the GLSS strategy, it is essential to integrate Green, Lean, and Six Sigma initiatives under the umbrella of GLSS. In this study, GLSS integration has been proposed based on intangible features like enablers, toolsets, etc. Management dedication, participation, and team effort have been recognized as the most remarkable enablers to implement GLSS in the manufacturing sector. Moreover, this study also proposes a five facet GLSS framework for the manufacturing sector to enhance organizational sustainability. The current work will facilitate managers and practitioners to execute a sustainable GLSS program in manufacturing firms for increased operational excellence as well as environmental sustainability

Water footprint assessment and its importance in Indian context: a meta-review

With the increasing population and per capita demand for freshwater, the burden of natural resources has increased many folds. Indian agriculture is still largely dependent on conventional methods of crop production that are mainly inefficient. Water security is essential for social and economic development, having an indispensable role in enhancing health, well-being, and economic progress, particularly in a developing country like India. Water footprint (WF) is an important indicator that helps ascertain the direct and indirect use of water in any process. WF modeling in agriculture enables us to pinpoint the impacts and limitations of the current crop production system. Assessing vulnerabilities across various regions and time helps us prepare actions to improve water productivity and promote sustainable water use. There is considerable spatial variability in blue, green, and gray WF among the different states of India due to their varied climate, soil, and topographic characteristics. WF assessment is critical for developing water allocation strategies, planning water trade, making policies, and implementing remedial measures. This paper describes the significance of WF and its proper management for sustainable crop production in India. HIGHLIGHTS Water security is essential for social and economic development, especially for countries like India, supporting nearly 17.1% of the world's population.; The average annual per capita water availability has declined over the years while demand from various sectors mounted over the years.; Among the various countries of the world, India has the largest total WF at 1047 billion cubic meters per year (BCM/year), followed by China (967 BCM/year) and the USA (826 BCM/year).

Effect of antioxidants supplementation on the quality of Beetal buck semen stored at 4°C

Aim: An experiment was designed to evaluate the role of Vitamin E and glutathione in improving the seminal parameters during hypothermic storage of liquid semen at 4°C for 72 h. Materials and Methods: Thirty-six semen ejaculates were collected by artificial vagina from 6 bucks (Beetal) during the normal reproduction season (September to November) at weekly interval. The samples were centrifuged, and the seminal plasma was removed. The sperm pellet was diluted with Tris-based extender and divided into three groups. Group T1: Control samples without antioxidants, Group T2: Samples supplemented with tocopherol at 3 mM, and Group T3: Samples supplemented with glutathione at 1 mM. The samples were evaluated for progressive motility, percent liveability, percent abnormal spermatozoa, and acrosome integrity after liquid preservation for 0, 24, 48, and 72 h. The level of lipid peroxidation and antioxidant enzymes, namely, glutathione peroxidase (GPx) and superoxide dismutase (SOD) were estimated after liquid preservation for 0 and 72 h. Results: It was observed that, after storage of semen at 4°C up to 72 h, the progressive sperm motility, percent liveability, percent abnormal spermatozoa, and percent intact acrosomes were significantly (p<0.05) higher in group T2 and T3 samples as compared to control. However, the level of lipid peroxidation in T2 and T3 groups was significantly (p<0.05) lower after 72 h of incubation at 4°C. Similarly, GPx and SOD values were significantly (p<0.05) increased in T2 and T3 groups after 72 h of storage at 4°C. Conclusion: Thus, it can be concluded that Vitamin E and glutathione supplementation at 3 mM and 1 mM, respectively, while preserving the semen samples at 4°C helped in maintaining the seminal parameters up to 72 h and protected the spermatozoa from oxidative damage

Discovery of Small Molecules from Echinacea angustifolia Targeting RNA-Dependent RNA Polymerase of Japanese Encephalitis Virus

The Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that causes viral encephalitis leading to neural damage, is a major threat in most Asian countries. The RNA-dependent RNA polymerase (RdRp) present in the viral genome is the key component for genome replication, making it an attractive target for antiviral drug development. In this study, the natural products from Echinacea angustifolia were retrieved for structure-based virtual screening against JEV&ndash;RdRp. The top six compounds (Echinacoside, Echinacin, Rutin, Cynaroside, Quercetagetin 7-glucoside, and Kaempferol-3-glucoside) were obtained based on the highest negative docking score, ADMET (absorption, distribution, metabolism, excretion, and toxicity), and molecular interaction. The computational analysis of these selected compounds against the co-crystallized ligands, i.e., ATP and GTP, were performed. Further, 100 ns molecular dynamic simulation and post-free binding energy calculation of all the selected compounds complexed with JEV&ndash;RdRP were performed to check the stability of the complexes. The obtained results showed considerable stability and intermolecular interaction with native ligand-binding site residues of JEV&ndash;RdRp. Hence, selected natural compounds are admissible inhibitors of JEV&ndash;RdRp protein and can be considered for future antiviral drug development studies

Implications of Microorganisms in Alzheimer&rsquo;s Disease

Alzheimer&rsquo;s disease (AD) is a deadly brain degenerative disorder that leads to brain shrinkage and dementia. AD is manifested with hyperphosphorylated tau protein levels and amyloid beta (A&beta;) peptide buildup in the hippocampus and cortex regions of the brain. The nervous tissue of AD patients also contains fungal proteins and DNA which are linked to bacterial infections, suggesting that polymicrobial infections also occur in the brains of those with AD. Both immunohistochemistry and next-generation sequencing (NGS) techniques were employed to assess fungal and bacterial infections in the brain tissue of AD patients and non-AD controls, with the most prevalent fungus genera detected in AD patients being Alternaria, Botrytis, Candida, and Malassezia. Interestingly, Fusarium was the most common genus detected in the control group. Both AD patients and controls were also detectable for Proteobacteria, followed by Firmicutes, Actinobacteria, and Bacteroides for bacterial infection. At the family level, Burkholderiaceae and Staphylococcaceae exhibited higher levels in the brains of those with AD than the brains of the control group. Accordingly, there is thought to be a viscous cycle of uncontrolled neuroinflammation and neurodegeneration in the brain, caused by agents such as the herpes simplex virus type 1 (HSV1), Chlamydophilapneumonia, and Spirochetes, and the presence of apolipoprotein E4 (APOE4), which is associated with an increased proinflammatory response in the immune system. Systemic proinflammatory cytokines are produced by microorganisms such as Cytomegalovirus, Helicobacter pylori, and those related to periodontal infections. These can then cross the blood&ndash;brain barrier (BBB) and lead to the onset of dementia. Here, we reviewed the relationship between the etiology of AD and microorganisms (such as bacterial pathogens, Herpesviridae viruses, and periodontal pathogens) according to the evidence available to understand the pathogenesis of AD. These findings might guide a targeted anti-inflammatory therapeutic approach to AD