Peningkatan Minat Dan Penguasaan Konsep Melalui Pembelajaran Learning Cycle 5e

This study aims to obtain an effective instructional model increase interest and concept’s mastery of XI science in acid-base of Arrhenius material. The population are the class of XI Science SMA Al Azhar 3 Bandar Lampung with XI IPA 2 as experiment class and XI 4 as control. This research method is a quasi-experimental pretest posttest control group design. The research’s results showed the average value of the index gain interest in the control and experimental classes respectively 0,34 and 0,49; and the mean index gain mastery of concepts for the control and experimental classes respectively 0,33 and 0,55. Based on hypothesis testing, it was concluded that learning through the class with LC 5E has an interest and mastery of concepts that is higher than learning conventional class. This suggests that the learning material through LC 5E is more effective in increasing students' interest and mastery of concepts.Penelitian ini bertujuan untuk memperoleh model pembelajaran yang afektif dalam meningkatkan minat dan penguasaan konsep asam-basa Arrhenius. Populasi dalam penelitian ini adalah selurush siswa kelas XI IPA SMA Al Azhar 3 Bandar Lampung dengan kelas XI IPA 2 sebagai kelas eksperimen dan XI IPA 4 kelas control. Metode penelitan ni adalah kuasi eksperimen dengan non equivalent control group design. Hasil penelitian menunjukkan nilai rerata indeks gain minat untuk kelas control dan eksperimen masing-masing 0,34 dan 0,49; dan rerata indeks gain penguasaan konsep untuk kelas kontrol dan eksperimen masing-masing 0,33 dan 0,55. Berdasarkan uji hipotesis menunjukkan bahwa pembelajaran menggunakan LC 5E lebih tinggi daripada pembelajaran kon-vensional. Hal ini menunjukkan bahwa pembelajaran dengan LC 5E lebih efektif dalam me-ningkatkan minat dan penguasaaan konsep siswa

Biología y ecología de cuatro especies medicinales de Gentianella recolectadas para el mercado en la Región Cajamarca, Perú

Se describió la biología y ecología de cuatro especies medicinales de Gentianella (Gentianaceae), con énfasis en aspectos relacionados con la producción, incluyendo el análisis de flavonoides totales. Todas las especies evaluadas fueron hierbas pequeñas. Gentianella graminea y G. crassicaulis son perennes y G. dianthoides y G. bicolor son anuales. Mientras que G. dianthoides produce abundantes semillas, las otras tienen muy escasa a moderada producción de semillas; todas con bajo poder germinativo. Gentianella crassicaulis produce hasta 90 g de materia seca/ planta, mientras que las restantes menos de 10 g de materia seca/planta. Gentianella graminea presentó niveles significativamente mayores de flavonoides totales en hoja y tallo comparado con G. dianthoides y G. crassicaulis (p < 0,05). Tres especies (G. graminea, G. crassicaulis y G. dianthoides) habitan en la región Jalca (2700 a 2850 m s.n.m.), asociadas a 30 especies (16 también medicinales); mientras que G. bicolor habita en la región Quechua (2600 a 2750 m s.n.m.), asociada a 17 especies (9 medicinales). Las cuatro especies tuvieron poblaciones escasas (≤ 2,5 plantas/m2 ) y su regeneración natural fue baja (1-4 plantas/m2 ). Gentianella dianthoides y G. crassicaulis se encuentran en estado vulnerable de conservación, por lo que se recomienda suspender su recolección

Antagonism of Tumoral Prolactin Receptor Promotes Autophagy-Related Cell Death

Therapeutic upregulation of macroautophagy in cancer cells provides an alternative mechanism forcell death. Prolactin (PRL) and its receptor (PRLR) are considered attractive therapeutic targets because of their roles as growth factors in tumor growth and progression. We utilized G129R, an antagonist peptide of PRL, to block activity of the tumoral PRL/PRLR axis, which resulted in inhibition of tumor growth in orthotopic models of human ovarian cancer. Prolonged treatment with G129R induced the accumulation of redundant autolysosomes in 3D cancer spheroids, leading to a type II programmed cell death. This inducible autophagy was a noncanonical beclin-1-independent pathway and was sustained by an astrocytic phosphoprotein (PEA-15) and protein kinase C zeta interactome. Lower levels of tumoral PRL/PRLR inclinical samples were associated with longer patient survival. Our findings provide an understanding of the mechanisms of tumor growth inhibition through targeting PRL/PRLR and may have clinical implications. © 2014 The Authors

Efficacy of futibatinib, an irreversible fibroblast growth factor receptor inhibitor, in FGFR-altered breast cancer.

Several alterations in fibroblast growth factor receptor (FGFR) genes have been found in breast cancer; however, they have not been well characterized as therapeutic targets. Futibatinib (TAS-120; Taiho) is a novel, selective, pan-FGFR inhibitor that inhibits FGFR1-4 at nanomolar concentrations. We sought to determine futibatinib\u27s efficacy in breast cancer models. Nine breast cancer patient-derived xenografts (PDXs) with various FGFR1-4 alterations and expression levels were treated with futibatinib. Antitumor efficacy was evaluated by change in tumor volume and time to tumor doubling. Alterations indicating sensitization to futibatinib in vivo were further characterized in vitro. FGFR gene expression between patient tumors and matching PDXs was significantly correlated; however, overall PDXs had higher FGFR3-4 expression. Futibatinib inhibited tumor growth in 3 of 9 PDXs, with tumor stabilization in an FGFR2-amplified model and prolonged regression (\u3e 110 days) in an FGFR2 Y375C mutant/amplified model. FGFR2 overexpression and, to a greater extent, FGFR2 Y375C expression in MCF10A cells enhanced cell growth and sensitivity to futibatinib. Per institutional and public databases, FGFR2 mutations and amplifications had a population frequency of 1.1%-2.6% and 1.5%-2.5%, respectively, in breast cancer patients. FGFR2 alterations in breast cancer may represent infrequent but highly promising targets for futibatinib

Autonomous Synthesis of Fluorescent Silica Biodots Using Engineered Fusion Proteins

Formation of biological materials is a well-controlled process that is orchestrated by biomolecules such as proteins. Proteins can control the nucleation and mineralization of biomaterials, thereby forming the hard tissues of biological organisms, such as bones, teeth, and shells. In this study, the design and implementation of multifunctional designer proteins are demonstrated for fluorescent silica micro/nanoparticle synthesis. The R5 motif of silaffin polypeptide, which is known for its silicification capability, was fused genetically into three spectrally distinct fluorescent proteins with the intention of forming modified fluorescent proteins. The bifunctional R5 peptide domain served as a tag to provide silica synthesis at ambient conditions. Three functional fusion constructs have been prepared, including GFPmut3-R5, Venus YFP-R5, and mCherry-R5. Recombinant fluorescent proteins were purified using silica-binding peptide tag through silica gel resin. Purified proteins were tested for their binding affinity to silica using quartz crystal microbalance with dissipation monitoring to make sure they can interact strong enough with the silica surfaces. Later, engineered fluorescent proteins were used to synthesize silica nano/microparticles using silica precursor materials. Synthesized silica particles were investigated for their fluorescence properties, including time-resolved fluorescence. Additionally, elemental analysis of the particles was carried out using electron energy loss spectroscopy and energy-filtered transmission electron microscopy. Last, they were tested for their biocompatibility. In this study, we aimed to provide a biomimetic route to synthesize fluorescent silica nanoparticles. Recombinant fluorescent proteins-directed silica nanoparticles synthesis offers a one-step, reliable method to produce fluorescent particles both for biomaterial applications and other nanotechnology applications

Multifunctional Protein-Enabled Patterning on Arrayed Ferroelectric Materials

This study demonstrates a biological route to programming well-defined protein-inorganic interfaces with an arrayed geometry via modular peptide tag technology. To illustrate this concept, we designed a model multifunctional fusion protein, which simultaneously displays a maltose-binding protein (MBP), a green fluorescence protein (GFPuv) and an inorganic-binding peptide (AgBP2C). The fused combinatorially selected AgBP2C tag controls and site-directs the multifunctional fusion protein to immobilize on silver nanoparticle arrays that are fabricated on specific domain surfaces of ferroelectric LiNbO₃ via photochemical deposition and in situ synthesis. Our combined peptide-assisted biological and ferroelectric lithography approach offers modular design and versatility in tailoring surface reactivity for fabrication of nanoscale devices in environmentally benign conditions