Effect of iron loading on isolated rat myocardium

2002-2003 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Hydrogen peroxide augments the injury effect of iron on the isolated rat heart and cardiomyocytes

2000-2001 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma

Aim: To reveal new tumor markers and target genes from differentially expressed genes of primary tumor samples using cDNA microarray. Methods: The 33P labeled cDNAs were synthesized by reverse transcription of message RNA from the liver cancerous tissue and adjacent non-cancerous liver tissue from the same patient and used to hybridize to LifeGrid 1.0 cDNA microarray blot containing 8400 known and unique human cDNA gene targets, and an expression profile of genes was produced in one paired human liver tumor tissue. After a global analysis of gene expression of 8400 genes, we selected some genes to confirm the differential expression using Northern blot and RT-PCR. Results: Parallel analysis of the hybridized signals enabled us to get an expression profile of genes in which about 500 genes were differentially expressed in the paired liver tumor tissues. We identified 4 genes, the expression of three (Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues. In addition, the expression of these genes in 6 hepatoma cell lines was also showed by RT-PCR analysis. Conclusion: cDNA microarray permits a high throughput identification of changes in gene expression. The genes encoding Beclin 1, RbAp48, Pir51 and aldolase b are first reported that may be related with hepatocarcinoma. Copyright © 2004 by The WJG Press ISSN 1007-9327.published_or_final_versio

Influence of interleukin-2 on Ca2+ handling in rat ventricular myocytes

In the present study, we examined the effect of interleukin-2 (IL-2) on cardiomyocyte Ca2+ handling. The effects of steady-state and transient changes in stimulation frequency on the intracellular Ca2+ transient were investigated in isolated ventricular myocytes by spectrofluorometry. In the steady state (0.2 Hz) IL-2 (200 U/ml) decreased the amplitude of Ca2+ transients induced by electrical stimulation and caffeine. At 1.25 mM extracellular Ca2+ concentration ([Ca 2+]o), when the stimulation frequency increased from 0.2 to 1.0 Hz, diastolic Ca2+ level and peak intracellular Ca 2+ concentration ([Ca2+]i), as well as the amplitude of the transient, increased. The positive frequency relationships of the peak and amplitude of [Ca2+]i transients were blunted in the IL-2-treated myocytes. The effect of IL-2 on the electrically induced [Ca2+]i transient was not normalized by increasing [Ca2+]o to 2.5 mM. IL-2 inhibited the frequency relationship of caffeine-induced Ca2+ release. Blockade of sarcoplasmic reticulum (SR) Ca2+-ATPase with thapsigargin resulted in a significant reduction of the amplitude-frequency relationship of the transient similar to that induced by IL-2. The restitutions were not different between control and IL-2 groups at 1.25 mM [Ca2+]o, which was slowed in IL-2-treated myocytes when [Ca2+]o was increased to 2.5 mM. There was no difference in the recirculation fraction (RF) between control and IL-2-treated myocytes at both 1.25 and 2.5 mM [Ca 2+]o. The effects of IL-2 on frequency relationship, restitution, and RF may be due to depressed SR functions and an increased Na+-Ca2+ exchange activity, but not to any change in L-type Ca2+ channels. © 2003 Elsevier Ltd. All rights reserved.postprin

A new statistical moment-based structural damage detection method

2008-2009 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Generalization of the statistical moment-based damage detection method

2010-2011 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

An iterative substructuring approach to the calculation of eigensolution and eigensensitivity

Author name used in this manuscript: You-Lin Xu2010-2011 > Academic research: refereed > Publication in refereed journalAccepted ManuscriptPublishe

Substructure based approach to finite element model updating

Author name used in this manuscript: You-Lin Xu2010-2011 > Academic research: refereed > Publication in refereed journalAccepted ManuscriptPublishe

Verification of a cable element for cable parametric vibration of one-cable-beam system subject to harmonic excitation and random excitation

Author name used in this publication: You-lin Xu2010-2011 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Improved substructuring method for eigensolutions of large-scale structures

Author name used in this manuscript: You-Lin Xu2008-2009 > Academic research: refereed > Publication in refereed journalAccepted ManuscriptPublishe