9,185 research outputs found
Synthetic biology routes to bio-artificial intelligence
The design of synthetic gene networks (SGNs) has advanced to the extent that novel genetic circuits are now being tested for their ability to recapitulate archetypal learning behaviours first defined in the fields of machine and animal learning. Here, we discuss the biological implementation of a perceptron algorithm for linear classification of input data. An expansion of this biological design that encompasses cellular 'teachers' and 'students' is also examined. We also discuss implementation of Pavlovian associative learning using SGNs and present an example of such a scheme and in silico simulation of its performance. In addition to designed SGNs, we also consider the option to establish conditions in which a population of SGNs can evolve diversity in order to better contend with complex input data. Finally, we compare recent ethical concerns in the field of artificial intelligence (AI) and the future challenges raised by bio-artificial intelligence (BI)
Bioresorbable scaffolds: a new paradigm in percutaneous coronary intervention
Numerous advances and innovative therapies have been introduced in interventional cardiology over the recent years, since the first introduction of balloon angioplasty, but bioresorbable scaffold is certainly one of the most exciting and attracting one. Despite the fact that the metallic drug-eluting stents have significantly diminished the re-stenosis ratio, they have considerable limitations including the hypersensitivity reaction to the polymer that can cause local inflammation, the risk of neo-atherosclerotic lesion formation which can lead to late stent failure as well as the fact that they may preclude surgical revascularization and distort vessel physiology. Bioresorbable scaffolds overcome these limitations as they have the ability to dissolve after providing temporary scaffolding which safeguards vessel patency. In this article we review the recent developments in the field and provide an overview of the devices and the evidence that support their efficacy in the treatment of CAD. Currently 3 devices are CE marked and in clinical use. Additional 24 companies are developing these kind of coronary devices. Most frequently used material is PLLA followed by magnesium
Real-Time Biosynthetic Reaction Monitoring Informs the Mechanism of Action of Antibiotics
\ua9 2024 The Authors. Published by American Chemical SocietyThe rapid spread of drug-resistant pathogens and the declining discovery of new antibiotics have created a global health crisis and heightened interest in the search for novel antibiotics. Beyond their discovery, elucidating mechanisms of action has necessitated new approaches, especially for antibiotics that interact with lipidic substrates and membrane proteins. Here, we develop a methodology for real-time reaction monitoring of the activities of two bacterial membrane phosphatases, UppP and PgpB. We then show how we can inhibit their activities using existing and newly discovered antibiotics such as bacitracin and teixobactin. Additionally, we found that the UppP dimer is stabilized by phosphatidylethanolamine, which, unexpectedly, enhanced the speed of substrate processing. Overall, our results demonstrate the potential of native mass spectrometry for real-time biosynthetic reaction monitoring of membrane enzymes, as well as their in situ inhibition and cofactor binding, to inform the mode of action of emerging antibiotics
Ring-like Oligomers of Synaptotagmins and Related C2 Domain Proteins
We recently reported that the C2AB portion of Synaptotagmin 1 (Syt1) could self-assemble into Ca2+-sensitive ring-like oligomers on membranes, which could potentially regulate neurotransmitter release. Here we report that analogous ring-like oligomers assemble from the C2AB domains of other Syt isoforms (Syt2, Syt7, Syt9) as well as related C2 domain containing protein, Doc2B and extended Synaptotagmins (E-Syts). Evidently, circular oligomerization is a general and conserved structural aspect of many C2 domain proteins, including Synaptotagmins. Further, using electron microscopy combined with targeted mutations, we show that under physiologically relevant conditions, both the Syt1 ring assembly and its rapid disruption by Ca2+ involve the well-established functional surfaces on the C2B domain that are important for synaptic transmission. Our data suggests that ring formation may be triggered at an early step in synaptic vesicle docking and positions Syt1 to synchronize neurotransmitter release to Ca2+ influx
Symbiosis between the TRECVid benchmark and video libraries at the Netherlands Institute for Sound and Vision
Audiovisual archives are investing in large-scale digitisation efforts of their analogue holdings and, in parallel, ingesting an ever-increasing amount of born- digital files in their digital storage facilities. Digitisation opens up new access paradigms and boosted re-use of audiovisual content. Query-log analyses show the shortcomings of manual annotation, therefore archives are complementing these annotations by developing novel search engines that automatically extract information from both audio and the visual tracks. Over the past few years, the TRECVid benchmark has developed a novel relationship with the Netherlands Institute of Sound and Vision (NISV) which goes beyond the NISV just providing data and use cases to TRECVid. Prototype and demonstrator systems developed as part of TRECVid are set to become a key driver in improving the quality of search engines at the NISV and will ultimately help other audiovisual archives to offer more efficient and more fine-grained access to their collections. This paper reports the experiences of NISV in leveraging the activities of the TRECVid benchmark
Computational and Mathematical Modelling of the EGF Receptor System
This chapter gives an overview of computational and mathematical modelling of the EGF receptor system. It begins with a survey of motivations for producing such models, then describes the main approaches that are taken to carrying out such modelling, viz. differential equations and individual-based modelling. Finally, a number of projects that applying modelling and simulation techniques to various aspects of the EGF receptor system are described
To VBAC or Not to VBAC
Catherine Spong discusses new research in PLoS Medicine that sheds more light on the risks of uterine rupture for women attempting a trial of labor following previous cesarean section
The Evolution of Data Fusion Methodologies Developed to Reconstruct Coronary Artery Geometry From Intravascular Imaging and Coronary Angiography Data: A Comprehensive Review
Understanding the mechanisms that regulate atherosclerotic plaque formation and
evolution is a crucial step for developing treatment strategies that will prevent plaque
progression and reduce cardiovascular events. Advances in signal processing and the
miniaturization of medical devices have enabled the design of multimodality intravascular
imaging catheters that allow complete and detailed assessment of plaque morphology
and biology. However, a significant limitation of these novel imaging catheters is that they
provide two-dimensional (2D) visualization of the lumen and vessel wall and thus they
cannot portray vessel geometry and 3D lesion architecture. To address this limitation
computer-based methodologies and user-friendly software have been developed. These
are able to off-line process and fuse intravascular imaging data with X-ray or computed
tomography coronary angiography (CTCA) to reconstruct coronary artery anatomy. The
aim of this review article is to summarize the evolution in the field of coronary artery
modeling; we thus present the first methodologies that were developed to model vessel
geometry, highlight the modifications introduced in revised methods to overcome the
limitations of the first approaches and discuss the challenges that need to be addressed,
so these techniques can have broad application in clinical practice and research
Quark Number Susceptibility with Finite Chemical Potential in Holographic QCD
We study the quark number susceptibility in holographic QCD with a finite
chemical potential or under an external magnetic field at finite temperature.
We first consider the quark number susceptibility with the chemical potential.
We observe that approaching the critical temperature from high temperature
regime, the quark number susceptibility divided by temperature square develops
a peak as we increase the chemical potential, which confirms recent lattice QCD
results. We discuss this behavior in connection with the existence of the
critical end point in the QCD phase diagram. We also consider the quark number
susceptibility under the external magnetic field. We predict that the quark
number susceptibility exhibits a blow-up behavior at low temperature as we
raise the value of the magnetic field. We finally spell out some limitations of
our study.Comment: 25 pages, 3 figures, published versio
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