3D simulations of an injection test done into an unsaturated porous and fractured limestone

We have developed a numerical model to represent the effect of injection test in unsaturated porous and fractured rock mass. The test was conducted at the LSBB (Laboratoire Souterrain à Bas Bruit) site close to Rustrel, Vaucluse, France in the field of the French ANR project called 'HPPP-CO2'. The results underline the impact of fractures on the hydro-mechanical response of the rock mass. Indeed fractures allow a faster dissipation of the water pressures and stress variations induced by the water injection. Back analysis lead us to also estimate the rock mass intrinsic permeability and compressibility of the injected laye

Hydraulic interactions between fractures and bedding planes in a carbonate aquifer studied by means of experimentally induced water-table fluctuations (Coaraze experimental site, southeastern France)

International audienceIn aquifers with variable permeability, the water exchanges between high and low permeability regions are controlled by the hydraulic head gradient. Past studies have addressed this problem mainly considering steadystate hydraulic conditions. To study such exchanges during water-table fluctuations, a spring was equipped with a water-gate that creates 10-meter artificial fluctuations of the water table. The water exchanges are discussed with respect to hydrochemical and pressure measurements in the groundwater. With successive water-table fluctuations the mineralization and pH decrease, but the bicarbonate content increases in response to carbon dioxide dissolution . At this scale of single fractures and surroundings, the hydrochemistry allows water flows from low or high permeability discontinuities to be discriminated. During hydraulic head fluctuations, the waters from low and high permeability discontinuities become mixed. During water-table rise, the low-permeability matrix contributed to the refilling of the permeable faults and to the mixing of the waters. Dynamic flows in the opposite direction to the hydraulic gradient can expel mineralized water, pushing it towards permeable discontinuities. This mechanism could be the cause of the peak in the mineralisation recorded in some karst springs at the start of flood events

Stabilization of fault slip by fluid injection in the laboratory and in situ

Faults can slip seismically or aseismically depending on their hydromechanical properties, which can be measured in the laboratory. Here, we demonstrate that fault slip induced by fluid injection in a natural fault at the decametric scale is quantitatively consistent with fault slip and frictional properties measured in the laboratory. The increase in fluid pressure first induces accelerating aseismic creep and fault opening. As the fluid pressure increases further, friction becomes mainly rate strengthening, favoring aseismic slip. Our study reveals how coupling between fault slip and fluid flow promotes stable fault creep during fluid injection. Seismicity is most probably triggered indirectly by the fluid injection due to loading of nonpressurized fault patches by aseismic creep

Extension de la méthode de cholesky pour la synthèse d'un signal 2-D à incréments autosimilaires 1

- Dans un contexte de synthèse de profil routier, cet article présente l'extension d'une méthode de synthèse monodimensionnelle à la génération d'un terrain fractal auto-similaire 2-D. L'objectif est de fournir un terrain dont la dimension fractale est isotrope en terme d'évolution spatiale. La méthode de synthèse s'appuie sur une décomposition de Cholesky. Nous montrons en outre le lien qu'il existe entre les matrices issues de la décomposition selon l'élongation longitudinale et transversale

First Year of Physics at CNGS

The CNGS facility (CERN Neutrinos to Gran Sasso) aims at directly detecting νμ→ντ neutrino oscillations [1]. An intense νμ beam (1017 νμ per day) is generated at CERN and directed over 732 km towards the Gran Sasso National Laboratory, LNGS, in Italy, where two large and complex detectors, OPERA and ICARUS, are located. Having resolved successfully some initial issues that occurred since its commissioning in 2006, that will be briefly summarized here, the facility had its first complete year of physics with 1.78×1019 protons extracted towards CNGS. The experiences gained in operating this 500 kW neutrino beam facility along with highlights of the beam performance in 2008 are discussed

First Year Physics at CNGS, presented at PAC09, Vancouver, Canada, 4-8 May 2009

The CNGS facility (CERN Neutrinos to Gran Sasso) aims at directly detecting νμ→ντ neutrino oscillations [1]. An intense νμ beam (1017 νμ per day) is generated at CERN and directed over 732 km towards the Gran Sasso National Laboratory, LNGS, in Italy, where two large and complex detectors, OPERA and ICARUS, are located. Having resolved successfully some initial issues that occurred since its commissioning in 2006, that will be briefly summarized here, the facility had its first complete year of physics with 1.78×10^19 protons extracted towards CNGS. The experiences gained in operating this 500 kW neutrino beam facility along with highlights of the beamperformance in 2008 are discussed

Hydromechanical modeling of pulse tests that measure fluid pressure and fracture normal displacement at the coaraze laboratory site

Abstract In situ fracture mechanical deformation and fluid flow interactions are investigated through a series of hydraulic pulse injection tests, using specialized borehole equipment that can simultaneously measure fluid pressure and fracture displacements. The tests were conducted in two horizontal boreholes spaced one meter apart vertically and intersecting a near-vertical highly permeable fault located within a shallow fractured carbonate rock. The field data were evaluated by conducting a series of coupled hydromechanical numerical analyses, using both distinct-element and finite-element modeling techniques and both two-and three-dimensional model representations that can incorporate various complexities in fracture network geometry. One unique feature of these pulse injection experiments is that the entire test cycle, both the initial pressure increase and subsequent pressure fall-off, is carefully monitored and used for the evaluation of the in situ hydromechanical behavior. Field test data are evaluated by plotting fracture normal displacement as a function of fluid pressure, measured at the same borehole. The resulting normal displacement-versus-pressure curves show a characteristic loop, in which the paths for loading (pressure increase) and unloading (pressure decrease) are different. By matching this characteristic loop behavior, the fracture normal stiffness and an equivalent stiffness (Young's modulus) of the surrounding rock mass can be back-calculated. Evaluation of the field tests by coupled numerical hydromechanical modeling shows that initial fracture hydraulic aperture and normal stiffness vary by a factor of 2 to 3 for the two 2 monitoring points within the same fracture plane. Moreover, the analyses show that hydraulic aperture and the normal stiffness of the pulse-tested fracture, the stiffness of surrounding rock matrix, and the properties and geometry of the surrounding fracture network significantly affect coupled hydromechanical responses during the pulse injection test. More specifically, the pressure-increase path of the normal displacement-versus-pressure curve is highly dependent on the hydromechanical parameters of the tested fracture and the stiffness of the matrix near the injection point, whereas the pressure-decrease path is highly influenced by mechanical processes within a larger portion of the surrounding fractured rock

Polycomb-mediated repression of EphrinA5 promotes growth and invasion of glioblastoma

Glioblastoma (GBM) is the most common and most aggressive intrinsic brain tumour in adults. Integrated transcriptomic and epigenomic analyses of glioblastoma initiating cells (GIC) in a mouse model uncovered a novel epigenetic regulation of EfnA5. In this model, Bmi1 enhances H3K27me3 at the EfnA5 locus and reinforces repression of selected target genes in a cellular context-dependent fashion. EfnA5 mediates Bmi1-dependent proliferation and invasion in vitro and tumour formation in an allograft model. Importantly, we show that this novel Polycomb feed-forward loop is also active in human GIC and we provide pre-clinical evidence of druggability of the EFNA5 signalling pathway in GBM xenografts overexpressing Bmi1

A Non-Commutative Extension of MELL

We extend multiplicative exponential linear logic (MELL) by a non-commutative, self-dual logical operator. The extended system, called NEL, is defined in the formalism of the calculus of structures, which is a generalisation of the sequent calculus and provides a more refined analysis of proofs. We should then be able to extend the range of applications of MELL, by modelling a broad notion of sequentiality and providing new properties of proofs. We show some proof theoretical results: decomposition and cut elimination. The new operator represents a significant challenge: to get our results we use here for the first time some novel techniques, which constitute a uniform and modular approach to cut elimination, contrary to what is possible in the sequent calculus

Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimens.

BackgroundTo determine differentially expressed and spliced RNA transcripts in chronic lymphocytic leukemia specimens a high throughput RNA-sequencing (HTS RNA-seq) analysis was performed.MethodsTen CLL specimens and five normal peripheral blood CD19+ B cells were analyzed by HTS RNA-seq. The library preparation was performed with Illumina TrueSeq RNA kit and analyzed by Illumina HiSeq 2000 sequencing system.ResultsAn average of 48.5 million reads for B cells, and 50.6 million reads for CLL specimens were obtained with 10396 and 10448 assembled transcripts for normal B cells and primary CLL specimens respectively. With the Cuffdiff analysis, 2091 differentially expressed genes (DEG) between B cells and CLL specimens based on FPKM (fragments per kilobase of transcript per million reads and false discovery rate, FDR q < 0.05, fold change >2) were identified. Expression of selected DEGs (n = 32) with up regulated and down regulated expression in CLL from RNA-seq data were also analyzed by qRT-PCR in a test cohort of CLL specimens. Even though there was a variation in fold expression of DEG genes between RNA-seq and qRT-PCR; more than 90 % of analyzed genes were validated by qRT-PCR analysis. Analysis of RNA-seq data for splicing alterations in CLL and B cells was performed by Multivariate Analysis of Transcript Splicing (MATS analysis). Skipped exon was the most frequent splicing alteration in CLL specimens with 128 significant events (P-value <0.05, minimum inclusion level difference >0.1).ConclusionThe RNA-seq analysis of CLL specimens identifies novel DEG and alternatively spliced genes that are potential prognostic markers and therapeutic targets. High level of validation by qRT-PCR for a number of DEG genes supports the accuracy of this analysis. Global comparison of transcriptomes of B cells, IGVH non-mutated CLL (U-CLL) and mutated CLL specimens (M-CLL) with multidimensional scaling analysis was able to segregate CLL and B cell transcriptomes but the M-CLL and U-CLL transcriptomes were indistinguishable. The analysis of HTS RNA-seq data to identify alternative splicing events and other genetic abnormalities specific to CLL is an added advantage of RNA-seq that is not feasible with other genome wide analysis