Minder mineralenverlies in de substraatteelt : een economische analyse van opties

De overheid wil stapsgewijs de lozingen van mineralen door glastuinbouwbedrijven met substraatteelt naar de omgeving terugdringen. In 2027 moeten deze lozingen vrijwel nul zijn. Onderzocht is welke maatregelen daartoe door de bedrijven genomen kunnen worden en welke kosten die met zich mee zullen brengen. The Dutch government wants to reduce the release (leaching) of nutrients from greenhouse horticultural firms with substrate-grown crops into the environment. These releases must be practically nil in 2027. This study examined which measures could be taken by the firms to comply with this objective and the associated costs

Fuel remains in archaeological context: expirimental and archaeological evidence for recognizing remains in hearts used by Iron Age farmers who lived in peatlands

Plant science

Purification and Ex Vivo Expansion of Fully Functional Salivary Gland Stem Cells

Hyposalivation often leads to irreversible and untreatable xerostomia. Salivary gland (SG) stem cell therapy is an attractive putative option to salvage these patients but is impeded by the limited availability of adult human tissue. Here, using murine SG cells, we demonstrate single-cell self-renewal, differentiation, enrichment of SG stem cells, and robust in vitro expansion. Dependent on stem cell marker expression, SG sphere-derived single cells could be differentiated in vitro into distinct lobular or ductal/lobular organoids, suggestive of progenitor or stem cell potency. Expanded cells were able to form miniglands/organoids containing multiple SG cell lineages. Expansion of these multipotent cells through serial passaging resulted in selection of a cell population, homogenous for stem cell marker expression (CD24hi/CD29hi). Cells highly expressing CD24 and CD29 could be prospectively isolated and were able to efficiently restore radiation-damaged SG function. Our approach will facilitate the use of adult SG stem cells for a variety of scientific and therapeutic purposes

Implementation of an anonymisation tool for clinical trials using a clinical trial processor integrated with an existing trial patient data information system

To present an adapted Clinical Trial Processor (CTP) test set-up for receiving, anonymising and saving Digital Imaging and Communications in Medicine (DICOM) data using external input from the original database of an existing clinical study information system to guide the anonymisation process. Two methods are presented for an adapted CTP test set-up. In the first method, images are pushed from the Picture Archiving and Communication System (PACS) using the DICOM protocol through a local network. In the second method, images are transferred through the internet using the HTTPS protocol. In total 25,000 images from 50 patients were moved from the PACS, anonymised and stored within roughly 2 h using the first method. In the second method, an average of 10 images per minute were transferred and processed over a residential connection. In both methods, no duplicated images were stored when previous images were retransferred. The anonymised images are stored in appropriate directories. The CTP can transfer and process DICOM images correctly in a very easy set-up providing a fast, secure and stable environment. The adapted CTP allows easy integration into an environment in which patient data are already included in an existing information system. Store DICOM images correctly in a very easy set-up in a fast, secure and stable environment Allows adaptation of the software to perform a certain task based on specific needs Allows easy integration into an existing environment Reduce the possibility of inappropriate anonymisation

Adolescent self-control predicts midlife hallucinatory experiences:40-year follow-up of a national birth cohort

Associations between self-control in adolescence and adult mental health are unclear in the general population; to our knowledge, no study has investigated self-control in relation to psychotic-like symptoms

GpaXItarl originating from Solanum tarijense is a major resistance locus to Globodera pallida and is localised on chromosome 11 of potato

Resistance to Globodera pallida Rookmaker (Pa3), originating from wild species Solanum tarijense was identified by QTL analysis and can be largely ascribed to one major QTL. GpaXItarl explained 81.3% of the phenotypic variance in the disease test. GpaXItarl is mapped to the long arm of chromosome 11. Another minor QTL explained 5.3% of the phenotypic variance and mapped to the long arm of chromosome 9. Clones containing both QTL showed no lower cyst counts than clones with only GpaXItarl. After Mendelising the phenotypic data, GpaXItarl could be more precisely mapped near markers GP163 and FEN427, thus anchoring GpaXItarl to a region with a known R-gene cluster containing virus and nematode resistance genes

Distinct evolution of colistin resistance associated with experimental resistance evolution models in Klebsiella pneumoniae

Pharmacolog