PEMERIKSAAN KOLESTROL PADA PENDERITA DIABETES MELITUS USIA 30-50 TAHUN DI RSUD GAYO LUES ACEH

Kolestrol merupakan komponen lemak yang terdapat pada pembuluh darah semua binatang dan juga manusia. Kolestrol sebenarnya berguna sebagai sumber energi, membentuk dinding sel-sel dalam tubuh dan sebagai bahan dasar pembentukan hormon-hormon steroid. Diabetes melitus adalah suatu sekumpulan gejala yang timbul pada seseorang yang disebabkan adanya peningkatan kadar glukosa darah akibat kekurangan insulin baik secra absolute maupun relative. Metabolism terganggu karena protein yang dipecah dalam proses glukogenesis berlebihan. Kolestrol dapat meningkat pada penderita Diabetes Melitus akibat adanya penumpukan komponen lemak atau sisa makanan yang berlebihan yang melewati ambang normal kolestrol yaitu lebih dari 200 mg/dl. Peneliti lakukan berjudul  Pemeriksaan Kolestrol Pada Penderita Diabetes Melitus Usia 30-50 Di RSUD Gayo Lues Aceh bertujuan untuk mengetahui Kadar Kolestrol Pada Penderita Diabtes Melitu. Desain penelitian ini bersifat Deskriptif Analitik dengan mengunakan metode Colorimetri di RSUD Gayo Lues Aceh. Berdasarkan penelitian yang dilakukan terhadap 20 sampel pasien yang menderita Diabetes Melitus yang melakukan pemeriksaan kadar Kolestrol mengalami peningkatan 50% (6 orang perempuan dan 4 orang laki-laki ) dan 50% normal (4 orang perempuan dan 6 orang laki-laki). Ditinjau dari hasil penelitian kadar kolestrol, maka pada penderita diabetes melitus disarankan tetap mengontrol kadar kolestrolnya dengan melakukan pemeriksaan laboratorium untuk menghindar berbagai penyakit lainnya dan menjaga pola kesehatan seperti menjaga pola makan dan olahraga teratur

PEMERIKSAAN JUMLAH SEL LEUKOSIT PADA PENDERITA TUBERKULOSIS PARU DI UPT KESEHATAN PARU MASYARAKAT DINAS KESEHATAN PROVINSI SUMATERA UTARA

Tuberkulosis paru atau sering disebut TB paru merupakan suatu penyakit infeksi paru yang disebabkan oleh kuman mycobacterium tuberculosis yang ditularkan melalui udara. Salah satu pemeriksaan penunjang pada penderita TB paru adalah pemeriksaan jumlah sel Lekosit. Lekosit berfungsi dalam memerangi infeksi yang disebabkan oleh virus, bakteri, ataupun proses metabolik toksin. Pada penderita TB paru ditemukan sedikit peningkatan jumlah sel Lekosit yang disebut dengan istilah Leukositosis. Tujuan peneltiian ini untuk mengetahui jumlah sel Lekosit pada penderita Tuberkulosis paru di UPT Kesehatan Paru Masyarakat Dinas Kesehatan Provinsi Sumatera Utara. Disain penelitian bersifat deskriftif cross sectional. Penulis ingin melakukan pemeriksaan jumlah sel Lekosit dengan menggunakan alat Auto Hematology Analyzer Mindray BC-300 Plus dengan sampel sebanyak 50 orang, bahan yang digunakan adalah darah dengan antikoagulan EDTA. Penelitian dilakukan di laboratorium UPT Kesehatan Paru Masyarakat Dinas Kesehatan Provinsi Sumatera Utara. Dari hasil penelitian diperoleh Lekosit yang normal sebanyak 30 orang (60%), meningkat sebanyak 20 orang (40%)

PEMERIKSAAN JUMLAH SEL LEUKOSIT PADA PENDERITA TUBERKULOSIS PARU DI UPT KESEHATAN PARU MASYARAKAT DINAS KESEHATAN PROVINSI SUMATERA UTARA

Tuberkulosis paru atau sering disebut TB paru merupakan suatu penyakit infeksi paru yang disebabkan oleh kuman mycobacterium tuberculosis yang ditularkan melalui udara. Salah satu pemeriksaan penunjang pada penderita TB paru adalah pemeriksaan jumlah sel Lekosit. Lekosit berfungsi dalam memerangi infeksi yang disebabkan oleh virus, bakteri, ataupun proses metabolik toksin. Pada penderita TB paru ditemukan sedikit peningkatan jumlah sel Lekosit yang disebut dengan istilah Leukositosis. Tujuan peneltiian ini untuk mengetahui jumlah sel Lekosit pada penderita Tuberkulosis paru di UPT Kesehatan Paru Masyarakat Dinas Kesehatan Provinsi Sumatera Utara. Disain penelitian bersifat deskriftif cross sectional. Penulis ingin melakukan pemeriksaan jumlah sel Lekosit dengan menggunakan alat Auto Hematology Analyzer Mindray BC-300 Plus dengan sampel sebanyak 50 orang, bahan yang digunakan adalah darah dengan antikoagulan EDTA. Penelitian dilakukan di laboratorium UPT Kesehatan Paru Masyarakat Dinas Kesehatan Provinsi Sumatera Utara. Dari hasil penelitian diperoleh Lekosit yang normal sebanyak 30 orang (60%), meningkat sebanyak 20 orang (40%)

Analysis of Biomass Briquettes Made from Bagasse Using Tapioca Starch Adhesive with Drying Temperature Variations

Dependency on fossil fuels is making their availability increasingly scarce. Renewable energy is considered the best solution to replace fossil fuels, and at present, the primary focus should be on optimizing the utilization of renewable energy sources. As an example, biomass briquettes are being manufactured using bagasse and tapioca flour as adhesive in a 2:3 ratio. This study utilized a compression force of 50 kgf/cm2 and variations in drying temperatures (100°C, 150°C, and 200°C) for 1 hour. The main objective of this research is to compare the quality of bagasse briquettes with the Indonesian National Standard (SNI) No. 01/6235/2000. The test results indicate that the best conditions were achieved at a drying temperature of 200°C, with a moisture content of 4.47%, a calorific value of 5077.32 cal/g, a combustion rate of 0.1386 g/min, and carbon, volatile matter, and ash content percentages of 22.8333%, 26.845%, and 2.8323%, respectively

Combined cardiological and neurological abnormalities due to filamin A gene mutation

Background: Cardiac defects can be the presenting symptom in patients with mutations in the X-linked gene FLNA. Dysfunction of this gene is associated with cardiac abnormalities, especially in the left ventricular outflow tract, but can also cause a congenital malformation of the cerebral cortex. We noticed that some patients diagnosed at the neurogenetics clinic had first presented to a cardiologist, suggesting that earlier recognition may be possible if the diagnosis is suspected. Methods and results: From the Erasmus MC cerebral malformations database 24 patients were identified with cerebral bilateral periventricular nodular heterotopia (PNH) without other cerebral cortical malformations. In six of these patients, a pathogenic mutation in FLNA was present. In five a cardiac defect was also found in the outflow tract. Four had presented to a cardiologist before the cerebral abnormalities were diagnosed. Conclusions: The cardiological phenotype typically consists of aortic or mitral regurgitation, coarctation of the aorta or other left-sided cardiac malformations. Most patients in this category will not have a FLNA mutation, but the presence of neurological complaints, hyperlaxity of the skin or joints and/or a family history with similar cardiac or neurological problems in a possibly X-linked pattern may alert the clinician to the possibility of a FLNA mutation

Maintenance treatment with quetiapine versus discontinuation after one year of treatment in patients with remitted first episode psychosis: randomised controlled trial

Objective To study rates of relapse in remitted patients with first episode psychosis who either continued or discontinued antipsychotic drugs after at least one year of maintenance treatment

Identifying house price diffusion patterns among Australian state capital cities

Prior research supports the proposition that house price diffusion shows a ripple effect along the spatial dimension. That is, house price changes in one region would reflect in subsequent house price changes in other regions, showing certain linkages among regions. Using the vector autoregression model and the impulse response function, this study investigates house price diffusion among Australia\u27s state capital cities, examining the response of one market to the innovation of other markets and determining the lagged terms for the maximum absolute value of the other markets\u27 responses. The results show that the most important subnational markets in Australia do not point to Sydney, rather towards Canberra and Hobart, while the Darwin market plays a role of buffer. The safest markets are Sydney and Melbourne. This study helps to predict house price movement trends in eight capital cities.<br /

Mucopolysaccharidosis type IIIB may predominantly present with an attenuated clinical phenotype

Mucopolysaccharidosis type IIIB (MPS IIIB, Sanfilippo syndrome type B) is a lysosomal storage disorder caused by deficiency of the enzyme N-acetyl-α-D-glucosaminidase (NAGLU). Information on the natural course of MPS IIIB is scarce but much needed in view of emerging therapies. To improve knowledge on the natural course, data on all 52 MPS IIIB patients ever identified by enzymatic studies in the Netherlands were gathered. Clinical data on 44 patients could be retrieved. Only a small number (n = 9; 21%) presented with a classical MPS III phenotype; all other patients showed a much more attenuated course of the disease characterized by a significantly slower regression of intellectual and motor abilities. The majority of patients lived well into adulthood. First signs of the disease, usually mild developmental delay, were observed at a median age of 4 years. Subsequently, patients showed a slowing and eventually a stagnation of development. Patients with the attenuated phenotype had a stable intellectual disability for many years. Molecular analysis was performed in 24 index patients. The missense changes p.R643C, p.S612G, p.E634K, and p.L497V were exclusively found in patients with the attenuated phenotype. MPS IIIB comprises a remarkably wide spectrum of disease severity, and an unselected cohort including all Dutch patients showed a large proportion (79%) with an attenuated phenotype. MPS IIIB must be considered in patients with a developmental delay, even in the absence of a progressive decline in intellectual abilities. A key feature, necessitating metabolic studies, is the coexistence of behavioral problems

Disassembly and reassembly of human papillomavirus virus-like particles produces more virion-like antibody reactivity

<p>Abstract</p> <p>Background</p> <p>Human papillomavirus (HPV) vaccines based on major capsid protein L1 are licensed in over 100 countries to prevent HPV infections. The yeast-derived recombinant quadrivalent HPV L1 vaccine, GARDASIL(R), has played an important role in reducing cancer and genital warts since its introduction in 2006. The L1 proteins self-assemble into virus-like particles (VLPs).</p> <p>Results</p> <p>VLPs were subjected to post-purification disassembly and reassembly (D/R) treatment during bioprocessing to improve VLP immunoreactivity and stability. The post-D/R HPV16 VLPs and their complex with H16.V5 neutralizing antibody Fab fragments were visualized by cryo electron microscopy, showing VLPs densely decorated with antibody. Along with structural improvements, post-D/R VLPs showed markedly higher antigenicity to conformational and neutralizing monoclonal antibodies (mAbs) H16.V5, H16.E70 and H263.A2, whereas binding to mAbs recognizing linear epitopes (H16.J4, H16.O7, and H16.H5) was greatly reduced.</p> <p>Strikingly, post-D/R VLPs showed no detectable binding to H16.H5, indicating that the H16.H5 epitope is not accessible in fully assembled VLPs. An atomic homology model of the entire</p> <p>HPV16 VLP was generated based on previously determined high-resolution structures of bovine papillomavirus and HPV16 L1 pentameric capsomeres.</p> <p>Conclusions</p> <p>D/R treatment of HPV16 L1 VLPs produces more homogeneous VLPs with more virion-like antibody reactivity. These effects can be attributed to a combination of more complete and regular assembly of the VLPs, better folding of L1, reduced non-specific disulfide-mediated aggregation and increased stability of the VLPs. Markedly different antigenicity of HPV16 VLPs was observed upon D/R treatment with a panel of monoclonal antibodies targeting neutralization sensitive epitopes. Multiple epitope-specific assays with a panel of mAbs with different properties and epitopes are required to gain a better understanding of the immunochemical properties of VLPs and to correlate the observed changes at the molecular level. Mapping of known antibody epitopes to the homology model explains the changes in antibody reactivity upon D/R. In particular, the H16.H5 epitope is partially occluded by intercapsomeric interactions involving the L1 C-terminal arm. The homology model allows a more precise mapping of antibody epitopes. This work provides a better understanding of VLPs in current vaccines and could guide the design of improved vaccines or therapeutics.</p