Millennial atmospheric CO2 changes linked to ocean ventilation modes over past 150,000 years

Ice core measurements show diverse atmospheric CO2 variations—increasing, decreasing or remaining stable—during millennial-scale North Atlantic cold periods called stadials. The reasons for these contrasting trends remain elusive. Ventilation of carbon-rich deep oceans can profoundly affect atmospheric CO2, but its millennial-scale history is poorly constrained. Here we present a well-dated high-resolution deep Atlantic acidity record over the past 150,000 years, which reveals five hitherto undetected modes of stadial ocean ventilation with different consequences for deep-sea carbon storage and associated atmospheric CO2 changes. Our data provide observational evidence to show that strong and often volumetrically extensive Southern Ocean ventilation released substantial amounts of deep-sea carbon during stadials when atmospheric CO2 rose prominently. By contrast, other stadials were characterized by weak ventilation via both Southern Ocean and North Atlantic, which promoted respired carbon accumulation and thus curtailed or reversed deep-sea carbon losses, resulting in diminished rises or even declines in atmospheric CO2. Our findings demonstrate that millennial-scale changes in deep-sea carbon storage and atmospheric CO2 are modulated by multiple ocean ventilation modes through the interplay of the two polar regions, rather than by the Southern Ocean alone, which is critical for comprehensive understanding of past and future carbon cycle adjustments to climate change

What Role Do Annelid Neoblasts Play? A Comparison of the Regeneration Patterns in a Neoblast-Bearing and a Neoblast-Lacking Enchytraeid Oligochaete

The term ‘neoblast’ was originally coined for a particular type of cell that had been observed during annelid regeneration, but is now used to describe the pluripotent/totipotent stem cells that are indispensable for planarian regeneration. Despite having the same name, however, planarian and annelid neoblasts are morphologically and functionally distinct, and many annelid species that lack neoblasts can nonetheless substantially regenerate. To further elucidate the functions of the annelid neoblasts, a comparison was made between the regeneration patterns of two enchytraeid oligochaetes, Enchytraeus japonensis and Enchytraeus buchholzi, which possess and lack neoblasts, respectively. In E. japonensis, which can reproduce asexually by fragmentation and subsequent regeneration, neoblasts are present in all segments except for the eight anterior-most segments including the seven head-specific segments, and all body fragments containing neoblasts can regenerate a complete head and a complete tail, irrespective of the region of the body from which they were originally derived. In E. japonensis, therefore, no antero-posterior gradient of regeneration ability exists in the trunk region. However, when amputation was carried out within the head region, where neoblasts are absent, the number of regenerated segments was found to be dependent on the level of amputation along the body axis. In E. buchholzi, which reproduces only sexually and lacks neoblasts in all segments, complete heads were never regenerated and incomplete (hypomeric) heads could be regenerated only from the anterior region of the body. Such an antero-posterior gradient of regeneration ability was observed for both the anterior and posterior regeneration in the whole body of E. buchholzi. These results indicate that the presence of neoblasts correlates with the absence of an antero-posterior gradient of regeneration ability along the body axis, and suggest that the annelid neoblasts are more essential for efficient asexual reproduction than for the regeneration of missing body parts

Glutamine synthetase gene expression during the regeneration of the annelid Enchytraeus japonensis

Enchytraeus japonensis is a highly regenerative oligochaete annelid that can regenerate a complete individual from a small body fragment in 4–5 days. In our previous study, we performed complementary deoxyribonucleic acid subtraction cloning to isolate genes that are upregulated during E. japonensis regeneration and identified glutamine synthetase (gs) as one of the most abundantly expressed genes during this process. In the present study, we show that the full-length sequence of E. japonensis glutamine synthetase (EjGS), which is the first reported annelid glutamine synthetase, is highly similar to other known class II glutamine synthetases. EjGS shows a 61–71% overall amino acid sequence identity with its counterparts in various other animal species, including Drosophila and mouse. We performed detailed expression analysis by in situ hybridization and reveal that strong gs expression occurs in the blastemal regions of regenerating E. japonensis soon after amputation. gs expression was detectable at the cell layer covering the wound and was found to persist in the epidermal cells during the formation and elongation of the blastema. Furthermore, in the elongated blastema, gs expression was detectable also in the presumptive regions of the brain, ventral nerve cord, and stomodeum. In the fully formed intact head, gs expression was also evident in the prostomium, brain, the anterior end of the ventral nerve cord, the epithelium of buccal and pharyngeal cavities, the pharyngeal pad, and in the esophageal appendages. In intact E. japonensis tails, gs expression was found in the growth zone in actively growing worms but not in full-grown individuals. In the nonblastemal regions of regenerating fragments and in intact worms, gs expression was also detected in the nephridia, chloragocytes, gut epithelium, epidermis, spermatids, and oocytes. These results suggest that EjGS may play roles in regeneration, nerve function, cell proliferation, nitrogenous waste excretion, macromolecule synthesis, and gametogenesis

The Lake CHAd Deep DRILLing project (CHADRILL) - targeting ~ 10 million years of environmental and climate change in Africa

At present, Lake Chad ( ~13°0 N, ~14° E) is a shallow freshwater lake located in the Sahel/Sahara region of central northern Africa. The lake is primarily fed by the Chari-Logone river system draining a ~600 000 km2 watershed in tropical Africa. Discharge is strongly controlled by the annual passage of the intertropical convergence zone (ITCZ) and monsoon circulation leading to a peak in rainfall during boreal summer. During recent decades, a large number of studies have been carried out in the Lake Chad Basin (LCB). They have mostly focused on a patchwork of exposed lake sediments and outcrops once inhabited by early hominids. A dataset generated from a 673m long geotechnical borehole drilled in 1973, along with outcrop and seismic reflection studies, reveal several hundred metres of Miocene-Pleistocene lacustrine deposits. CHADRILL aims to recover a sedimentary core spanning the Miocene-Pleistocene sediment succession of Lake Chad through deep drilling. This record will provide significant insights into the modulation of orbitally forced changes in northern African hydroclimate under different climate boundary conditions such as high CO2 and absence of Northern Hemisphere ice sheets. These investigations will also help unravel both the age and the origin of the lake and its current desert surrounding. The LCB is very rich in early hominid fossils (Australopithecus bahrelghazali; Sahelanthropus tchadensis) of Late Miocene age. Thus, retrieving a sediment core from this basin will provide the most continuous climatic and environmental record with which to compare hominid migrations across northern Africa and has major implications for understanding human evolution. Furthermore, due to its dramatic and episodically changing water levels and associated depositional modes, Lake Chad's sediments resemble maybe an analogue for lake systems that were once present on Mars. Consequently, the study of the subsurface biosphere contained in these sediments has the potential to shed light on microbial biodiversity present in this type of depositional environment. We propose to drill a total of ~1800m of poorly to semi-consolidated lacustrine, fluvial, and eolian sediments down to bedrock at a single on-shore site close to the shoreline of present-day Lake Chad. We propose to locate our drilling operations on-shore close to the site where the geotechnical Bol borehole (13°280 N, 14°440 E) was drilled in 1973. This is for two main reasons: (1) nowhere else in the Chad Basin do we have such detailed information about the lithologies to be drilled; and (2) the Bol site is close to the depocentre of the Chad Basin and therefore likely to provide the stratigraphically most continuous sequence

Rock magnetic detection of distal ice-rafted debries: clue for the identification of Heinrich layers on the Portuguese margin (vol 180, pg 61, 2000)

High resolution measurements of concentration and grain size dependent magnetic parameters along a sedimentary sequence deposited on the Portuguese margin (38 degrees N) during the last 120 ka allowed the detection of multiple thin layers slightly enriched by a coarse lithogenic fraction. Sizes, mineralogy, shapes and mode of deposition identify the material as ice-rafted detritus (IRD). The four most recent events dated by calibrated radiocarbon ages are contemporaneous with Heinrich events HE1-HE4. Older events can be identified as HE5, HE6 or with minor IRD that occurred during stage 5, The resolution and sensitivity obtained with rock magnetic methods enable the detection of multiple secondary events showing that iceberg delivery was a frequent phenomenon directly linked to temperature variations over ice caps; Heinrich events representing the extreme cases. (C) 2000 Elsevier Science B.V. All rights reserved

Early Pleistocene presence of Acheulian hominins in South India

South Asia is rich in Lower Paleolithic Acheulian sites. These have been attributed to the Middle Pleistocene on the basis of a small number of dates, with a few older but disputed age estimates. Here, we report new ages from the excavated site of Attirampakkam, where paleomagnetic measurements and direct Al-26/Be-10 burial dating of stone artifacts now position the earliest Acheulian levels as no younger than 1.07 million years ago (Ma), with a pooled average age of 1.51 +/- 0.07 Ma. These results reveal that, during the Early Pleistocene, India was already occupied by hominins fully conversant with an Acheulian technology including handaxes and cleavers among other artifacts. This implies that a spread of bifacial technologies across Asia occurred earlier than previously accepted