Groundwater Vulnerability Assessment of the Tarkwa Mining Area Using SINTACS Approach and GIS

Groundwater vulnerability assessment to delineate areas that are susceptible to contamination from mining and anthropogenic activities has become an important element for resource management and landuse planning. In view of the extensive mining in the Tarkwa area, quality of groundwater has become an important issue. This study estimates aquifer vulnerability by applying the SINTACS model which uses seven environmental parameters to evaluate aquifer vulnerability and geographical information system (GIS) in the Tarkwa mining area. Sensitivity analysis has also been carried out to evaluate the relative importance of the model parameters for aquifer vulnerability. The SINTACS model results show that the intrusive rocks within the Tarkwaian and the Birimian rocks are dominated by very high vulnerability classes while the Banket Series is characterised by high vulnerability class. The Huni Sandstones have moderately high vulnerability. In addition, the Kawere Group and the Tarkwa Phyllites displayed medium vulnerability. Analysis from the variogram model shows that all parameters used in the SINTACS model have a strong spatial structure. From statistical analysis, depth to water parameter inflicted the highest impact on the vulnerability of the aquifer followed by effective infiltration, vadose zone media, soil media, aquifer media, topography and hydraulic conductivity in the order of decreasing impact. Sensitivity analysis indicated that the aquifer media, hydraulic characteristics and topography cause large variation in vulnerability index. Depth to water and effective infiltration were found to be more effective in assessing aquifer vulnerability. Keywords: Groundwater, Vulnerability, Tarkwa, SINTACS, GI

Combined Electrical Resistivity Imaging and Electromagnetic Survey for Groundwater Studies in the Tarkwa Mining Area, Ghana

The major source of potable water in Tarkwa is the Bonsa Treatment Plant sourced from the Bonsa River. The activities of illegal miners along the banks of the Bonsa River has resulted in pollution of the river. This has resulted in high treatment cost and irregular supply of water to the Tarkwa Township and surrounding communities that are fed by the Bonsa Treatment Plant. In view of the difficulty in getting frequent and regular potable supply of water, people have resorted to construction of boreholes and hand-dug wells. However, the success rates and borehole yields are low especially in the hydrogeologically difficult terrains in the Tarkwa area. The aim of this paper is to investigate the hydrogeological conditions of the Tarkwa area using both the Electrical Resistivity Imaging (ERI) and Electromagnetic (EM) geophysical techniques to determine the electrical resistivity and conductivity values that are related to groundwater accumulation, so that potential water-bearing zones can be identified. Results from electrical resistivity show that the general resistivity distribution in the Tarkwa area is between 32 Ωm and 100 000 Ωm. Water-bearing zones in the Huni Sandstone occur to a depth of 35 m with an average resistivity value of 400 Ωm, at a depth of 60 m and a resistivity of 600 Ωm in the Tarkwa Phyllite, at a depth of 55 m and a resistivity of 600 Ωm in the Banket Series and 50 m depth with resistivity value of 500 Ωm in the Kawere Conglomerate respectively. The electromagnetic conductivity values also show that the general conductivity distribution in the Tarkwa area is 3 – 32 S/m. The application of electrical resistivity and electromagnetic techniques separately gives success rate of 80 % and 65 % respectively. An improved success rate of 86 % is achieved combining the two techniques. Keywords: Electrical Resistivity Imaging, Electromagnetic Method, Groundwate

Product Marking and Conformity Assessment of Portland Cements on the Ghanaian Market

Cement bound concrete materials and complementary fittings are requisite ingredients for all civil engineering works. In all these, Portland cement, a basic binding ingredient for the concrete work is the dominant binder. In Ghana, there are various brands of cement on the market. Five major brand products currently in circulation include the Ghana Cement (GHACEM), Western DIAMOND Cement (DIAMOND), CIMAF Cement, DANGOTE Cement and SUPACEM Cement. Increased infrastructural development has placed high demand on cement consumption. Consequently, new products keep emerging in the market. Indeed, a standard measure to provide product marking and evaluations of conformity to standard Class thresholds are required for the desired specification, properties and the performance quality of the cement products. This research therefore sets to ascertain the strength quality of the five cement brands on the Ghanaian market by checking their conformity to C-30 and C-40 standard compressive tests, using their 32.5-R and 42.5-R flagship brands. To achieve this, concrete cubes were moulded with fixed mix ratio of 1:1⅟2:3 and 1:1:2 for C-30 and C-40 respectively. To achieve the desired strength conformity, the slump as well as the coarse and fine aggregate constituents were standardised. The results indicated that the cement brands despite parading same strength thresholds in the market, do not exhibit same strength build-up. There are significant variations in growth of compressive strength over time. It was observed also that conformance threshold within 28 days was not attained for a number of the brands. Indeed, not until 56 days or more some of the brands could not achieve their desired compressive strength thresholds

Estimation of the Quantity of Water in the Abandoned Underground Mine of Gold Fields Ghana Limited Tarkwa: A Potential Source to Augment Water Supply to Tarkwa Municipality

The Tarkwa district is an important gold mining area in the Southwestern part of Ghana. The main source of potable water supply to the Tarkwa Nsuaem Municipality is from the Bonsa River treatment plant managed by the Ghana Water Company Limited (GWCL). The River is under threat from serious contamination by illegal mining ("galamsey") activities within its catchment area. Consequently, the amount of water supplied to the Municipality has not kept pace with its growing population due to increasing treatment cost and supply difficulties. The need to find alternative and sustainable sources of potable water supply to augment that from GWCL to the Municipality has become imperative. A large void volume created as a result of the abandoned underground mine operated by Gold Fields Ghana Limited (GFGL), after its closure in 1999 has flooded. This potential water resource is being pumped out daily, and wasted, sometimes spilling-over to low lying areas around the mine when allowed to reach its decant level. This study estimated the quantity of water in the Abontiakoon Vertical Shaft (AVS) which is part of the large underground void using survey production figures and post-closure void filling parameters resulting in 2.8 x 106 m3 and 2.9 x 106 m3 respectively. The rate of recharge to the underground water was also estimated to ascertain the sustainability of the void water should it be considered for use by employing the model of predicting rebound on “void filling” basis and average dewatering rate before closure at 2 535 m3/day and 2 618 m3/day respectively; indicating that recharge to the AVS reservoir is about 6 x 106 gal/day or 30% of current daily water supply deficit in the TNM. The estimated potential volume of mine water in storage in the entire Tarkwa underground void is 32 x106 m3. Two samples of the mine water were taken in November 2011 and February 2015 for quality analysis, in order to have a fair knowledge of the water quality parameters. The quality of the underground water was found to be potentially good, and not likely to cause any health threats, or water quality problems. Depth sampling is recommended to determine the chemical profile of the reservoir. Keywords: Reservoir, Municipality, Bonsa River, Contamination, Tarkw

The Geometry and Structural Analysis of the Gold Deposits of Chirano Mine

The Chirano Mine gold deposit is a typical example of a structurally controlled deposit developed along the Kumasi Basin and the Sefwi Belt margin structure. The area has undergone various regimes of structural deformations. Consequently, all the Chirano deposits are intimately associated with shears and faults along a single continuous structural corridor known as the Chirano Shear Zone (CSZ). The CSZ geometry has been categorised into three major zones namely: (i) Laminated veins in shears, (ii) Breccia and (iii) Ductile to brittle ductile zones. The shear veins trend NE-SW and N-S, are laminated and occur in the sheared fabric close to the footwall. Penetrative foliated zones varying from a few centimeters to several meters constitute the ductile to brittle-ductile structures. Gold grades are much higher within this zone. Analysis of cataclasis intensity recorded in drill core confirms a semi brittle form of deformation within the mineralised domain. The CSZ has different orientations in dip and strike from the south of the mining lease to the north. The subtle changes in orientation are deposit dependent. The structure has a sinuous shape and tends to pinch and swell. The current geometry and the distribution of stratigraphy and orebodies at Chirano is a manifestation of the complex interplay of magmatic and hydrothermal events in the area.  Keywords: Ductile, Brittle-Ductile, Breccia, Chirano Shear Zone, Chirano Lode Horizo

Typhoid fever outbreak in the Democratic Republic of Congo: Case control and ecological study

During 2011 a large outbreak of typhoid fever affected an estimated 1430 people in Kikwit, Democratic Republic of Congo. The outbreak started in military camps in the city but then spread to the general population. This paper reports the results of an ecological analysis and a case-control study undertaken to examine water and other possible transmission pathways. Attack rates were determined for health areas and risk ratios were estimated with respect to spatial exposures. Approximately 15 months after the outbreak, demographic, environmental and exposure data were collected for 320 cases and 640 controls residing in the worst affected areas, using a structured interview questionnaire. Unadjusted and adjusted odds ratios were estimated. Complete data were available for 956 respondents. Residents of areas with water supplied via gravity on the mains network were at much greater risk of disease acquisition (risk ratio = 6.20, 95%CI 3.39-11.35) than residents of areas not supplied by this mains network. In the case control study, typhoid was found to be associated with ever using tap water from the municipal supply (OR = 4.29, 95% CI 2.20-8.38). Visible urine or faeces in the latrine was also associated with increased risk of typhoid and having chosen a water source because it is protected was negatively associated. Knowledge that washing hands can prevent typhoid fever, and stated habit of handwashing habits before cooking or after toileting was associated with increased risk of disease. However, observed associations between handwashing or plate-sharing with disease risk could very likely be due to recall bias. This outbreak of typhoid fever was strongly associated with drinking water from the municipal drinking water supply, based on the descriptive and analytic epidemiology and the finding of high levels of faecal contamination of drinking water. Future outbreaks of potentially waterborne disease need an integrated response that includes epidemiology and environmental microbiology during early stages of the outbreak

Responses of phytoplankton assemblages to iron availability and mixing water masses during the spring bloom in the Oyashio region, NW Pacific

Spring phytoplankton blooms play a major role in the carbon biogeochemical cycle of the Oyashio region, western subarctic Pacific, where the seasonal biological drawdown effect on seawater pCO 2 is one of the greatest among the world\u27s oceans. However, the bloom often terminates before depleting macronutrients, and the initiation and magnitude of the bloom is heterogeneous. We conducted a high resolution taxonomic and physiological assessment of phytoplankton in relation to the different physicochemical water masses of Coastal Oyashio Water (COW), Oyashio water (OYW), and modified Kuroshio water (MKW) in the Oyashio region from April to June 2007. Massive diatom blooms were found in April. Then, chlorophyll a concentration, cell abundance of diatom taxa, and the maximum photosystem II photochemical efficiency (F v /F m ) were positively correlated with the mixing ratios of COW, suggesting that the spring bloom in April was strongly affected by the intrusion of COW. In the OYW, intensive blooms occurred from the middle of May under low dissolved iron (DFe) concentration (\u3c 0.26 nM). Redundancy analysis showed that while diatom blooms accompanied by COW were related to DFe concentration, this was not the case in the OYW. These results indicated that diatoms in the OYW possess different iron adaptation strategies compared with diatoms in the water masses affected by COW. This led to the spatial heterogeneity of the Oyashio spring bloom. The results presented here demonstrate that water mass characterization with detailed assessments of phytoplankton taxonomy and physiological status can improve our understanding of marine ecosystems

DRAM-3 modulates autophagy and promotes cell survival in the absence of glucose

Macroautophagy is a membrane-trafficking process that delivers cytoplasmic constituents to lysosomes for degradation. The process operates under basal conditions as a mechanism to turnover damaged or misfolded proteins and organelles. As a result, it has a major role in preserving cellular integrity and viability. In addition to this basal function, macroautophagy can also be modulated in response to various forms of cellular stress, and the rate and cargoes of macroautophagy can be tailored to facilitate appropriate cellular responses in particular situations. The macroautophagy machinery is regulated by a group of evolutionarily conserved autophagy-related (ATG) proteins and by several other autophagy regulators, which either have tissue-restricted expression or operate in specific contexts. We report here the characterization of a novel autophagy regulator that we have termed DRAM-3 due to its significant homology to damage-regulated autophagy modulator (DRAM-1). DRAM-3 is expressed in a broad spectrum of normal tissues and tumor cells, but different from DRAM-1, DRAM-3 is not induced by p53 or DNA-damaging agents. Immunofluorescence studies revealed that DRAM-3 localizes to lysosomes/autolysosomes, endosomes and the plasma membrane, but not the endoplasmic reticulum, phagophores, autophagosomes or Golgi, indicating significant overlap with DRAM-1 localization and with organelles associated with macroautophagy. In this regard, we further proceed to show that DRAM-3 expression causes accumulation of autophagosomes under basal conditions and enhances autophagic flux. Reciprocally, CRISPR/Cas9-mediated disruption of DRAM-3 impairs autophagic flux confirming that DRAM-3 is a modulator of macroautophagy. As macroautophagy can be cytoprotective under starvation conditions, we also tested whether DRAM-3 could promote survival on nutrient deprivation. This revealed that DRAM-3 can repress cell death and promote long-term clonogenic survival of cells grown in the absence of glucose. Interestingly, however, this effect is macroautophagy-independent. In summary, these findings constitute the primary characterization of DRAM-3 as a modulator of both macroautophagy and cell survival under starvation conditions