Application of biotechnology in fish breeding. II: production of highly immune genetically modified redbelly tilapia, Tilapia zillii

The present study aims to produce a highly immune, genetically modified redbelly tilapia, Tilapia zillii with accelerated growth as a result of direct injection of shark (Squalus acanthias L.) DNA into skeletal muscles of fish fingerlings at concentrations of 10, 20, 40 and 80 μg/fish. The results showed that the fish injected with 40 μg/fish had significant (P&le0.05) superiority of growth performance. Also, the body composition of these fish was improved. The fish injected with 20 and 40 μg/fish had significant (P&le0.05) higher means of total antibody activity (total IgM), serum total protein and globulin compared with other injected groups. Cytological examination for all injected fish and their control revealed the same diploid number (2n=44) of chromosomes, and no chromosomal abberations were detected. Moreover, DNA fingerprinting showed high polymorphism among injected fish. Therefore, variable fragments of shark DNA may randomly integrated into T. zillii muscle genomes. The present investigation revealed also that egg diameters of injected females and their control were divided into nine groups, which varied between 0.2 and 1.7 mm. In addition, ovary of T. zillii females injected with 10; 20; 40 and 80 μg/fish of shark DNA showed 10, 38, 65 and 18% normal oocytes, respectively. Also, test of males injected with various amounts of DNA showed large number of abnormalities. Moreover, the comparison between all injected fish revealed that the testes and ovaries of fish injected with 80 μg/fish were more deformed and atretic. This means that the effects of intramuscular direct injection of foreign DNA into T. zillii could be limited to germ cells of fish. Therefore, further studies about the establishment of these effects on the following generations are needed. The result indicates a possible easy and rapid way for improving fish characteristics.African Journal of Biotechnology Vol. 4 (5), pp. 449-459, 200

Cycle-finite module categories

We describe the structure of module categories of finite dimensional algebras over an algebraically closed field for which the cycles of nonzero nonisomorphisms between indecomposable finite dimensional modules are finite (do not belong to the infinite Jacobson radical of the module category). Moreover, geometric and homological properties of these module categories are exhibited

Structure of the Deformed 7075 Aircraft Al - Alloy with Material Analysis Using Diffraction (MAUD)

This work aims to study the effect of plastic deformation on the 7075 Al - alloy using Material Analyses Using Diffraction (MAUD). Plastic deformation produces dislocation defects. Using a hydraulic press, samples were deformed up to 25%. The XRD was measured for each degree of deformation. The MAUD program was used to analyze the data, and mathematical methods were deployed to understand the various behaviors observed. The lattice parameter, crystallite size, average internal stress, micro-strain, and dislocation density of the 7075Al-alloy were calculated. As the deformation degree increased, there was a progressive decline in the crystallite size as an increase in the micro-strain and dislocation density. The flow stress changes from 5.8 to 49 MPa and the stored dislocation energy varies from 2.62 to 185 kPa

Tilted algebras and short chains of modules

We provide an affirmative answer for the question raised almost twenty years ago concerning the characterization of tilted artin algebras by the existence of a sincere finitely generated module which is not the middle of a short chain

Microwave-Assisted Solvothermal Synthesis of Mo-Doped TiO2 with Exceptional Textural Properties and Superior Adsorption Kinetics

Assigned to their outstanding physicochemical properties, TiO2-based materials have been studied in various applications. Herein, TiO2 doped with different Mo contents (Mo-TiO2) was synthesized via a microwave-assisted solvothermal approach. This was achieved using titanium (IV) butoxide and molybdenum (III) chloride as a precursor and dodecylamine as a surface directing agent. The uniform effective heating delivered by microwave heating reduced the reaction time to less than 30 min, representing several orders of magnitude lower than conventional heating methods. The average particle size ranged between 9.7 and 27.5 nm and it decreased with increasing the Mo content. Furthermore, Mo-TiO2 revealed mesoporous architectures with a high surface area ranging between 170 and 260 m2 g−1, which is superior compared to previously reported Mo-doped TiO2. The performance of Mo-TiO2 was evaluated towards the adsorption of Rhodamine B (RhB). In contrast to TiO2, which revealed negligible adsorption for RhB, Mo-doped samples depicted rapid adsorption for RhB, with a rate that increased with the increase in Mo content. Additionally, Mo-TiO2 expressed enhanced adsorption kinetics for RhB compared to state-of-the-art adsorbents. The introduced synthesis procedure holds a grand promise for the versatile synthesis of metal-doped TiO2 nanostructures with outstanding physicochemical properties.NPRP Grant no. NPRP 12S-0304-190218 from the Qatar National Research Fund (a member of the Qatar Foundation). The statements made herein are solely the responsibility of the authors

High gamma activity distinguishes frontal cognitive control regions from adjacent cortical networks.

Though the lateral frontal cortex is broadly implicated in cognitive control, functional MRI (fMRI) studies suggest fine-grained distinctions within this region. To examine this question electrophysiologically, we placed electrodes on the lateral frontal cortex in patients undergoing awake craniotomy for tumor resection. Patients performed verbal tasks with a manipulation of attentional switching, a canonical control demand. Power in the high gamma range (70-250 Hz) distinguished electrodes based on their location within a high-resolution fMRI network parcellation of the frontal lobe. Electrodes within the canonical fronto-parietal control network showed increased power in the switching condition, a result absent in electrodes within default mode, language and somato-motor networks. High gamma results contrasted with spatially distributed power decreases in the beta range (12-30 Hz). These results confirm the importance of fine-scale functional distinctions within the human frontal lobe, and pave the way for increased precision of functional mapping in tumor surgeries

4d N=2 Gauge Theories and Quivers: the Non-Simply Laced Case

We construct the BPS quivers with superpotential for the 4d N=2 gauge theories with non-simply laced Lie groups (B_n, C_n, F_4 and G_2). The construction is inspired by the BIKMSV geometric engineering of these gauge groups as non-split singular elliptic fibrations. From the categorical viewpoint of arXiv:1203.6743, the fibration of the light category L(g) over the (degenerate) Gaiotto curve has a monodromy given by the action of the outer automorphism of the corresponding unfolded Lie algebra. In view of the Katz--Vafa `matter from geometry' mechanism, the monodromic idea may be extended to the construction of (Q, W) for SYM coupled to higher matter representations. This is done through a construction we call specialization.Comment: 42 pages, 2 figure

Tumour-infiltrated cortex participates in large-scale cognitive circuits.

The extent to which tumour-infiltrated brain tissue contributes to cognitive function remains unclear. We tested the hypothesis that cortical tissue infiltrated by diffuse gliomas participates in large-scale cognitive circuits using a unique combination of intracranial electrocorticography (ECoG) and resting-state functional magnetic resonance (fMRI) imaging in four patients. We also assessed the relationship between functional connectivity with tumour-infiltrated tissue and long-term cognitive outcomes in a larger, overlapping cohort of 17 patients. We observed significant task-related high gamma (70-250 Hz) power modulations in tumour-infiltrated cortex in response to increased cognitive effort (i.e., switch counting compared to simple counting), implying preserved functionality of neoplastic tissue for complex tasks probing executive function. We found that tumour locations corresponding to task-responsive electrodes exhibited functional connectivity patterns that significantly co-localised with canonical brain networks implicated in executive function. Specifically, we discovered that tumour-infiltrated cortex with larger task-related high gamma power modulations tended to be more functionally connected to the dorsal attention network (DAN). Finally, we demonstrated that tumour-DAN connectivity is evident across a larger cohort of patients with gliomas and that it relates to long-term postsurgical outcomes in goal-directed attention. Overall, this study contributes convergent fMRI-ECoG evidence that tumour-infiltrated cortex participates in large-scale neurocognitive circuits that support executive function in health. These findings underscore the potential clinical utility of mapping large-scale connectivity of tumour-infiltrated tissue in the care of patients with diffuse gliomas

The Role of Transporters in the Pharmacokinetics of Orally Administered Drugs

Drug transporters are recognized as key players in the processes of drug absorption, distribution, metabolism, and elimination. The localization of uptake and efflux transporters in organs responsible for drug biotransformation and excretion gives transporter proteins a unique gatekeeper function in controlling drug access to metabolizing enzymes and excretory pathways. This review seeks to discuss the influence intestinal and hepatic drug transporters have on pharmacokinetic parameters, including bioavailability, exposure, clearance, volume of distribution, and half-life, for orally dosed drugs. This review also describes in detail the Biopharmaceutics Drug Disposition Classification System (BDDCS) and explains how many of the effects drug transporters exert on oral drug pharmacokinetic parameters can be predicted by this classification scheme

Distribution Patterns of E-Cadherin, Type VII Collagen and Fibronectin in Denture-Related Stomatitis: A Preliminary Study

The distribution of epithelial E-cadherin, basement membrane type VII collagen, and underlying connective tissues fibronectin were investigated immunohistochemically and compared in normal palatal mucosa and in denture-related stomatitis (DRS) derivatives using monoclonal antibodies.Biopsies of palatal mucosa were obtained from twelve patients enrolled in this study, 8 with type II DRS and 4 with healthy mucosa