Effects of artificial substrates on the growth, survival and spatial distribution of Litopenaeus vannamei in the intensive culture condition

In order to investigate the effects of artificial substrates (vertical surface of polypropylene fabrics) on cultured shrimp, we reared 28-day old Litopenaeus vannamei post-larvae (PL28) intensively for 90 days at a density of 510 shrimp/m^2 in each of 8 tanks. Two tanks containing no artificial substrate were a control group, and 1, 3 and 5 artificial substrates were present in other 6 tanks. The volume of each tank was 100 L. In the tanks with artificial substrates, the percentage of shrimp distribution on the bottom was less significant (P<0.05) than that in the control tanks. The percentage of shrimps attached to the artificial substrates increased and fewer shrimp occupied the tank bottom as more artificial substrates were added to the tanks. Moreover the trends were more significant as rearing days increased. These results showed that artificial substrates could disperse the shrimp from the tank bottom onto the artificial substrates and thus alleviate the negative effect of high stocking density on shrimp growth in the tanks. Both the average weight and survival in the tanks with artificial substrates were significantly higher (P<0.05) than those in the control tanks. Furthermore, weight and survival increased when more artificial substrates were added. Because the shrimps in all tanks were supplied with suitable water quality and adequate nutritional food, we suggest that the differences of growth and survival were affected mainly by living space added with the addition of artificial substrates

Effect of vitamin K2 on bone mineral density and serum cathepsin K in female osteoporosis patients

Purpose: To investigate the influence of vitamin K2 on bone mineral density, bone metabolism and serum tissue protease K (cathepsin K) in female patients with osteoporosis.Method: A total of 210 osteoporosis patients in Affiliated Hospital of Nanjing University of Chinese Medicine who met the inclusion criteria were selected from January 2017 to January 2018. The patients were randomly divided into vitamin K2 group, strontium renate group and blank control group (70 patients/group). Strontium ranelate group was orally given 2 g of strontium ranelate daily, while vitamin K2 group received 15 mg of Gulikang capsule 3 times a day. Bone mineral density (BMD) and serum osteocalcin (BGP), β-collagen degradation product (β-crosslaps), type I procollagen amino terminal propeptide (PINP), cathepsin K (cathe K) and TRAP were measured prior to drug treatment, and six months after surgery, using standard procedures.Results: Relative to the blank control, hip and lumbar spine density of vitamin K2 and strontium ranelate groups increased to varying degrees. Strontium ranelate group had significantly higher bone mineral density (BMD) than any other groups (p &lt; 0.05), and also had the lowest osteoclast activity (β-crosslaps and TRAP) and the highest osteogenic activity (BGP and PINP). On the other hand, osteoclast and osteogenic activities increased significantly (p &lt; 0.05) in the vitamin K2 group.Conclusion: Appropriate vitamin K2 treatment improves BMD in the hip and waist of women with osteoporosis by promoting osteogenic activity, and by reducing osteoclast activity and cathepsin K expression.Keywords: Osteoporosis, Bone mineral density, Strontium ranelate, Vitamin K

Mitigating Nitrous Oxide Emissions from Tea Field Soil Using Bioaugmentation with a Trichoderma viride

Land-use conversion from woodlands to tea fields in subtropical areas of central China leads to increased nitrous oxide (N2O) emissions, partly due to increased nitrogen fertilizer use. A field investigation of N2O using a static closed chamber-gas chromatography revealed that the average N2O fluxes in tea fields with 225 kg N ha−1 yr−1 fertilizer application were 9.4 ± 6.2 times higher than those of woodlands. Accordingly, it is urgent to develop practices for mitigating N2O emissions from tea fields. By liquid-state fermentation of sweet potato starch wastewater and solid-state fermentation of paddy straw with application of Trichoderma viride, we provided the tea plantation with biofertilizer containing 2.4 t C ha−1 and 58.7 kg N ha−1. Compared to use of synthetic N fertilizer, use of biofertilizer at 225 kg N ha−1 yr−1 significantly reduced N2O emissions by 33.3%–71.8% and increased the tea yield by 16.2%–62.2%. Therefore, the process of bioconversion/bioaugmentation tested in this study was found to be a cost-effective and feasible approach to reducing N2O emissions and can be considered the best management practice for tea fields

Association between plasma trimethylamine N -oxide and neoatherosclerosis in patients with very late stent thrombosis

Abstract(#br)Background(#br)Trimethylamine N -oxide (TMAO) has been shown to promote the development of atherosclerosis. However, the relationship between plasma TMAO and neoatherosclerosis, an important underlying mechanism of very late stent thrombosis (VLST), is unknown.(#br)Methods(#br)This post hoc study investigated the association between TMAO and neoatherosclerosis in two independent cohorts. These included a control group of 50 healthy volunteers and a study cohort of 50 patients with VLST who presented with ST-segment elevation myocardial infarction and underwent optical coherence tomography examination. Of the 50 patients with VLST, 23 had neoatherosclerosis and 27 did not have neoatherosclerosis. Patients with neoatherosclerosis were further divided into two subgroups, including 14 patients with plaque rupture and 9 without plaque rupture.(#br)Results(#br)The plasma TMAO levels, detected using mass spectrometry, were significantly higher in patients with VLST than in healthy individuals (median [interquartile range]: 2.50 [1.67-3.84] vs. 1.32 [0.86-2.44] μM; P < 0.001). Among the VLST patients, the plasma TMAO levels were significantly higher in patients with neoatherosclerosis than in those without neoatherosclerosis (3.69 [2.46-5.29] vs. 1.96 [1.39-2.80] μM; P<0.001). In addition, in patients with neoatherosclerosis, patients with plaque rupture had significantly higher plasma TMAO concentrations than those without plaque rupture (4.51 [3.41-5.85] vs. 2.46 [2.05-3.55] μM; P=0.005). Multivariate analysis indicated that TMAO was an independent predictor of neoatherosclerosis (odds ratio 3.41; 95% confidence interval: 1.59-7.30; P=0.002). Moreover, the area under the receiver operating characteristic curve for TMAO, differentiated by neoatherosclerosis, was 0.85.(#br)Conclusions(#br)Plasma TMAO was significantly correlated with neoatherosclerosis and plaque rupture in patients with VLST

SHON expression predicts response and relapse risk of breast cancer patients after anthracycline-based combination chemotherapy or tamoxifen treatment

BACKGROUND: SHON nuclear expression (SHON-Nuc+) was previously reported to predict clinical outcomes to tamoxifen therapy in ERα+ breast cancer (BC). Herein we determined if SHON expression detected by specific monoclonal antibodies could provide a more accurate prediction and serve as a biomarker for anthracycline-based combination chemotherapy (ACT).METHODS: SHON expression was determined by immunohistochemistry in the Nottingham early-stage-BC cohort (n=1,650) who, if eligible, received adjuvant tamoxifen; the Nottingham ERα- early-stage-BC (n=697) patients who received adjuvant ACT; and the Nottingham locally advanced-BC cohort who received pre- operative ACT with/without taxanes (Neo-ACT, n=120) and if eligible, 5-year adjuvant tamoxifen treatment. Prognostic significance of SHON and its relationship with the clinical outcome of treatments were analysed.RESULTS: As previously reported, SHON-Nuc+ in high risk/ERα+ patients was significantly associated with a 48% death risk reduction after exclusive adjuvant tamoxifen treatment compared with SHON-Nuc- [HR(95%CI)=0.52(0.34-0.78), p=0.002]. Meanwhile, in ERα- patients treated with adjuvant ACT, SHON cytoplasmic expression (SHON-Cyto+) was significantly associated with a 50% death risk reduction compared with SHON-Cyto- [HR(95%CI)=0.50(0.34-0.73), p=0.0003]. Moreover, in patients received Neo-ACT, SHON-Nuc- or SHON-Cyto+ was associated with an increased pathological complete response (pCR) compared with SHON-Nuc+ [21% vs 4%; OR(95%CI)=5.88(1.28-27.03), p=0.012], or SHON-Cyto- [20.5% vs 4.5%; OR(95%CI)=5.43(1.18-25.03), p=0.017], respectively. After receiving Neo-ACT, patients with SHON-Nuc+ had a significantly lower distant relapse risk compared to those with SHON-Nuc- [HR(95%CI)=0.41(0.19-0.87), p=0.038], whereas SHON-Cyto+ patients had a significantly higher distant relapse risk compared to SHON-Cyto- patients [HR(95%CI)=4.63(1.05-20.39), p=0.043]. Furthermore, multivariate Cox regression analyses revealed that SHON-Cyto+ was independently associated with a higher risk of distant relapse after Neo-ACT and 5- year tamoxifen treatment [HR(95%CI)=5.08(1.13-44.52), p=0.037]. The interaction term between ERα status and SHON-Nuc+ (p=0.005), and between SHON-Nuc+ and tamoxifen therapy (p=0.007), were both statistically significant.CONCLUSION: SHON-Nuc+ in tumours predicts response to tamoxifen in ERα+ BC while SHON-Cyto+ predicts response to ACT

Facile Preparation of Loose P84 Copolyimide/GO Composite Membrane with Excellent Selectivity and Solvent Resistance

In this study, multilayer graphene oxide (GO) was used to prepare the functional layer of polyimide/GO composite membrane with polyimide (P84) used as the supporting layer. Chitosan added in the functional layer was utilized to adjust the selectivity of the composite membrane. The effects of GO and chitosan contents on membrane morphology and separation performance were investigated in detail. The composite membrane showed high rejection to Congo red and Methyl orange with high flux but low rejection to Na2SO4 and MgCl2 at 0.2 MPa and ambient temperature. The membrane exhibited excellent solvent resistance in N,N-dimethylacetamide (DMAc) after being crosslinked with 0.5 wt.% triethylene tetramine. The result means that a highly selective and solvent-resistant P84/GO composite membrane was prepared with the facile filtration preparation method

Two new brackish-water species of **Testudinella** (Rotifera: Testudinellidae) from Qiao Island in the Pearl River estuary, China, with a key to marine and brackish-water **Testudinella**

FIGURE 1. Location of the sampling sites on Qi'ao Island in the Pearl River estuary, Guangdong, China. Shadow area stands for mangrove forest; black circles represent the sampling sites, AL: artificial lake site; MS: mangrove swamp site; TR: tidal river sitePublished as part of Wei, Nan, De, Willem H. & Xu, Runlin, 2011, Two new brackish-water species of Testudinella (Rotifera: Testudinellidae) from Qi'ao Island in the Pearl River estuary, China, with a key to marine and brackish-water Testudinella, pp. 41-56 in Zootaxa 3051 on page 42, DOI: 10.5281/zenodo.20778