Coupled steel slag and biochar amendment correlated with higher methanotrophic abundance and lower CH₄ emission in subtropical paddies

Aerobic methanotrophs in paddies serve as methane (CH₄) filters and thereby reduce CH₄ emissions. Amending soil with waste products can mitigate CH₄ emissions in crops, but little is known about the impacts of amendments with steel slag and biochar on the populations and activities of aerobic methanotrophs in rice cropland. We used real-time quantitative PCR detecting system and high-throughput sequencing to determine the effects of slag and biochar amendments on CH₄ emission, abundance, and community structure of methanotrophs, and the relationships between soil properties and the abundance and community composition of methanotrophs during the rice growing season in both early and late paddies. Soil salinity and pH were significantly higher for an amendment with both slag and biochar than the control in both the early and late paddies, and pH was significantly higher for a slag amendment in the late paddy. Cumulative CH₄ emission was lower for the slag and slag + biochar amendments than the control in early paddy by-34.1%. Methanotrophic abundance was three- and sixfold higher for the slag + biochar amendment than the control in the early and late paddies (p < 0.05), respectively. The abundance of different groups of methanotrophs varied among the treatments. The relative abundance of Methylosarcina was higher for the slag amendment than the control, and the relative abundance of Methylomonas was lower for biochar, and slag + biochar amendments than the control. The relative abundance of Methylocystis was higher for the slag and slag + biochar amendments than the control in the early paddy, and the relative abundance of Methylocystis was higher for the slag, biochar, and slag + biochar amendments in the late paddy. Univariate and multivariate analyses indicated that the higher abundance of methanotrophic bacteria for the slag and slag + biochar amendments was correlated with soil pH, salinity, soil organic carbon, and C/N ratio, and the relative abundances of Methylocystis, Methylomonas, and Methylosarcina were associated with the effective mitigation of CH₄ emission in the paddies. A discriminant general analysis indicated that the total population of methanotrophs was larger for the slag + biochar amendment than the control, and that this effect was only weakly correlated with changes in the soil properties, demonstrating that this effect on the size and species composition of methanotrophic soil populations was mostly associated with a direct effect of the slag + biochar amendmen

Study on recovery of copper and zinc from cyanide lean solution in a smelting company

In the process of cyanidation of gold concentrate from a smelting company, the content of copper and zinc in the liquid gradually increased. In production, acidizing process is used to treat the lean solution from high copper concentrate, and mixed products containing copper, zinc and other metals are produced. In this paper, the pH value of zinc precipitation is 6.0 and the pH value of copper precipitation is 3.0 through theoretical calculation and experiment. The separation of copper and zinc in the lean solution is realized successfully. The zinc product with grade of 42.97% and the copper product with grade of 58.33% are produced

Sustained release formulation of an anti-tuberculosis drug based on para-amino salicylic acid-zinc layered hydroxide nanocomposite

Background: Tuberculosis (TB), is caused by the bacteria, Mycobacterium tuberculosis and its a threat to humans since centuries. Depending on the type of TB, its treatment can last for 6-24 months which is a major cause for patients non-compliance and treatment failure. Many adverse effects are associated with the currently available TB medicines, and there has been no new anti-tuberculosis drug on the market for more than 50 year, as the drug development is very lengthy and budget consuming process.Development of the biocompatible nano drug delivery systems with the ability to minimize the side effects of the drugs, protection of the drug from enzymatic degradation. And most importantly the drug delivery systems which can deliver the drug at target site would increase the therapeutic efficacy. Nanovehicles with their tendency to release the drug in a sustained manner would result in the bioavalibilty of the drugs in the body for a longer period of time and this would reduce the dosing frequency in drug administration. The biocompatible nanovehicles with the properties like sustained release of drug of the target site, protection of the drug from physio-chemical degradation, reduction in dosing frequency, and prolong bioavailability of drug in the body would result in the shortening of the treatment duration. All of these factors would improve the patient compliance with chemotherapy of TB.Result: An anti-tuberculosis drug, 4-amino salicylic acid (4-ASA) was successfully intercalated into the interlamellae of zinc layered hydroxide (ZLH) via direct reaction with zinc oxide suspension. The X-ray diffraction patterns and FTIR analyses indicate that the molecule was successfully intercalated into the ZLH interlayer space with an average basal spacing of 24 Å. Furthermore, TGA and DTG results show that the drug 4-ASA is stabilized in the interlayers by electrostatic interaction. The release of 4-ASA from the nanocomposite was found to be in a sustained manner. The nanocomposite treated with normal 3T3 cells shows it reduces cell viability in a dose- and time-dependent manner.Conclusions: Sustained release formulation of the nanocomposite, 4-ASA intercalated into zinc layered hydroxides, with its ease of preparation, sustained release of the active and less-toxic to the cell is a step forward for a more patient-friendly chemotherapy of Tuberculosis

Data from: A high-efficiency hydrocyclone designed by response surface methodology for acid hydrolysis residue recycling

A high-efficiency hydrocyclone was designed by response surface methodology to evaluate the recycling of acid hydrolysis residues from titanium dioxide (TiO2) production as a study case. TiO2 is an important product and the world's best white pigment. During its production from ilmenite (FeTiO3) by the sulphuric acid method, the incomplete reaction produces large amounts of residue, which also contain unreacted ilmenite. Large amounts of residue are generally accumulated without any treatment. Hydrocycloneuse is regarded as a method for separating and recovering chemicals from process residues by which the unreacted components can be recycled efficiently. However, hydrocyclones designed by conventional procedures may have some limitations regarding classification sharpness. In this paper, numerical experiments and laboratory tests were performed to evaluate the classification sharpness of various hydrocyclone designs. Response surface methodology was used to optimize hydrocyclones with different structural configurations. Based on the response models, a designed hydrocyclone with a high sharpness of classification of particles was constructed. The sharpness of the newly designed hydrocyclone increased from 80.5 % to 93.3 %. The vortex finder separated approximately 89.9 % of the fine particles in impurities, while 51.0 % of TiO2was recycled by the spigot. The hydrocyclone proposed in this paper properly minimizes the risk of environmental pollution caused by TiO2 production and provides a significant estimated cost savings

Dynamic Analysis of the Complex Motion of Three-Section Cudgel in Wushu Sports

Purpose: To provide some suggestions on how to effectively master these movements during training. Methods: The dynamics method and ABAQUS simulation analysis were used to analyze the two technical movements of the three-section cudgels: wrestling cudgels and retrieving cudgels. Results: In the process of wrestling the cudgel, the best effect is achieved when the height of the cudgel holding hand is between 70 cm and 80 cm from the ground. The maximum height of the rebound was very similar with different initial angular velocities, at 4.5–9 cm. The initial angular velocities caused significant impact to the horizontal movement of cudgel at 8 s. By excluding the errors, the horizontal movement of cudgel increased approximately linearly with the increase of the initial angular velocity. Conclusions: When the height between held cudgel and the ground was controlled at 1.5 times the height of the middle section, the rebound of the tail section was the least. When completing the movement of three-section cudgel, the cudgel body should be in the same plane perpendicular to the ground and to better retrieve the three-section cudgel. The main factor affecting the cudgel wrestling was the height between the cudgel holder and the ground

Terrestrial Water Storage Component Changes Derived from Multisource Data and Their Responses to ENSO in Nicaragua

Approximately 3.5 million people in Nicaragua have experienced food insecurity due to the El Niño-Southern Oscillation (ENSO)-induced drought from 2014 to 2016. It is essential to study terrestrial water storage component (TWSC) changes and their responses to ENSO to prevent the water crisis in Nicaragua influenced by ENSO. In this paper, we investigate the TWSC changes in Nicaragua and its sub-basins derived from the Gravity Recovery and Climate Experiment (GRACE)’s temporal gravity field, hydrological model, and water level data, and then determine the connection between the TWSC and ENSO from April 2002 to April 2021 by time series analysis. The research results show that: (1) The estimated TWSC changes in Nicaragua are in good agreement with the variation of precipitation and evaporation, and precipitation is the main cause of TWSC variation. (2) According to the cross-correlation analysis, there is a significant negative peak correlation between the interannual TWSC and ENSO in western Nicaragua, especially for interannual soil moisture (−0.80). The difference in peak correlation between the western and eastern sub-basins may be due to the topographic hindrance of the ENSO-inspired precipitation process. (3) The cross-wavelet analysis indicates that the resonance periods between TWSC and ENSO are primarily 2 and 4 years. These resonance periods are related to the two ENSO modes (the central Pacific (CP) mode with a quasi-2-year period and the eastern Pacific (EP) mode with a quasi-4-year period). Furthermore, their resonance phase variation may be due to the transition to ENSO mode. This study revealed the relationship between ENSO and TWSC in Nicaragua, which can provide a certain reference for water resources regulation