Experimental study on damage characteristics of Beishan granite under single loading and multiple loading with AE techniques

Abstract An insight into the damage characteristics of host rock is important for permanent disposal of high-level radioactive waste (HLW). Single loading and multiple loading tests for Beishan granite were carried out. The acoustic emission (AE) monitoring system was utilized simultaneously to measure the damage evolution in the specimens. The results show that, during single loading the AE signal has been maintained at a very high level after dilatancy. But a decreasing trend of AE signals can be observed when the axial stress approaches the peak stress. While during multiple loading, AE signals are mainly generated in the loading period in the elastic stage. However, in the failure and residual stages, AE signals generated in the unloading period are considerable. Most of the AE waveforms have a dominant frequency of less than 200 kHz and amplitude of less than 80 dB, and three frequency bands are obtained. In addition, the amplitude increases with the increase of loading stress. Furthermore, the obtained AE b-values indicate that the relative proportion of micro- to macro-cracks increases first and then decreases with increasing confining pressure. And the AE a-values and b-values confirms that micro cracks develop rapidly in the damage stage and lead to the formation of macro cracks in the residual stage for the specimens experienced multiple loading

Multidisciplinary Geophysical Investigations over Deep Coal-Bearing Strata: A Case Study in Yangjiazhangzi, Northeast China

With the majority of coal mines in uncovered and semi-covered coal strata now explored and developed, most of the undiscovered coal-bearing strata are concealed. Compared with expensive drilling, deep targets such as concealed coal-bearing strata can be more efficiently and cost-effectively discovered through geophysical methods. We designed an integrated geophysical exploration approach to detect coal-bearing strata in the Yangjiazhangzi (YJZZ) area. Large-scale magnetotellurics (MT) is used to describe the geological structure along with its profile through the YJZZ area. An aeromagnetic survey was used to delineate the spatial distribution characteristics of the YJZZ syncline, a coal-bearing prospect tectonic unit. Localized exploration with controlled-source audio magnetotellurics (CSAMT) and MT reveals coal-bearing targets for drilling. Drilling results verified the targets identified through the integrated geophysical approach. Coal-bearing strata in the Benxi formation, the Taiyuan formation, and the Shanxi formation of the Permo-Carboniferous age are found between 630 and 770 m. This case study demonstrates that the multidisciplinary geophysical strategy can provide reliable results and credible data interpretation for deep coal seam resources exploration. The findings of this study can provide reference for explorers to carry out their specific exploration cases

Structural Characteristics and the Antioxidant and Hypoglycemic Activities of a Polysaccharide from <i>Lonicera caerulea</i> L. Pomace

In this study, a novel polysaccharide, LPP, was obtained from Lonicera caerulea L. pomace by ultrasonic-assisted heating and was purified by Sephadex G-100. The structural characteristics of LPP showed that the molecular weight (Mw) was 8.53 × 104 Da; that it was mainly composed of galacturonic acid, followed by galactose; that it possessed the characteristic functional groups of polysaccharides; and that it had an absence of O-glycosidic bonds and crystalline and triple helix structures. Furthermore, LPP exhibited a favorable thermodynamic stability and antioxidant, hypoglycemic, and hypolipidemic activities in a dose-dependent manner in vitro, demonstrating that LPP can be used as an agent to regulate glycolipid metabolism. Additionally, the relationship between its bio-activities is discussed in this paper. The results revealed that the RP, •OH, and NO2− radicals had synergistic promoting effects, and polysaccharides with a strong antioxidant ability may have excellent hypoglycemic and hypolipidemic effects. Collectively, these results suggest that LPP has a strong bio-activity, and that Lonicera caerulea L. pomace can be used as a potential polysaccharide source

Structural Characteristics and the Antioxidant and Hypoglycemic Activities of a Polysaccharide from Lonicera caerulea L. Pomace

In this study, a novel polysaccharide, LPP, was obtained from Lonicera caerulea L. pomace by ultrasonic-assisted heating and was purified by Sephadex G-100. The structural characteristics of LPP showed that the molecular weight (Mw) was 8.53 &times; 104 Da; that it was mainly composed of galacturonic acid, followed by galactose; that it possessed the characteristic functional groups of polysaccharides; and that it had an absence of O-glycosidic bonds and crystalline and triple helix structures. Furthermore, LPP exhibited a favorable thermodynamic stability and antioxidant, hypoglycemic, and hypolipidemic activities in a dose-dependent manner in vitro, demonstrating that LPP can be used as an agent to regulate glycolipid metabolism. Additionally, the relationship between its bio-activities is discussed in this paper. The results revealed that the RP, &bull;OH, and NO2&minus; radicals had synergistic promoting effects, and polysaccharides with a strong antioxidant ability may have excellent hypoglycemic and hypolipidemic effects. Collectively, these results suggest that LPP has a strong bio-activity, and that Lonicera caerulea L. pomace can be used as a potential polysaccharide source

Dynamics of a Partially Confined, Vertical Upward-Fluid-Conveying, Slender Cantilever Pipe with Reverse External Flow

A linear theoretical model is established for the dynamics of a hanging vertical cantilevered pipe which is subjected concurrently to internal and reverse external axial flows. Such pipe systems may have instability by flutter (amplified oscillations) or static divergence (buckling). The pipe system under consideration is a slender flexible cantilevered pipe hanging concentrically within an inflexible external pipe of larger diameter. From the clamped end to the free end, fluid is injected through the annular passage between the external pipe and the cantilevered pipe. When exiting the annular passage, the fluid discharges in the counter direction along the cantilevered pipe. The inflexible external pipe has a variable length and it can cover a portion of the length of the cantilevered pipe. This pipe system has been applied in the solution mining and in the salt cavern underground energy storage industry. The planar motion equation of the system is solved by means of a Galerkin method, and Euler&#8211;Bernoulli beam eigenfunctions are used as comparison functions. Calculations are conducted to quantify the effects of different confinement conditions (i.e., the radial confinement degree of the annular passage and the confined-flow length) on the cantilevered pipe stability, for a long leaching-tubing-like system. For a long system, an increase in the radial confinement degree of the annular passage and the confined-flow length gives rise to a series of flutter and divergence. Additionally, the effect of the cantilevered pipe length is studied. Increasing the cantilevered pipe length results in an increase of the critical flow velocity while a decrease of the associated critical frequency. For a long enough system, the critical frequency almost disappears

Membrane remodeling properties of the Parkinson’s disease protein LRRK2

Mutations in Leucine-rich repeat kinase 2 (LRRK2) are responsible for late-onset autosomal dominant Parkinson’s disease. LRRK2 has been implicated in a wide range of physiological processes including membrane repair in the endolysosomal system. Here, using cell-free systems, we report that purified LRRK2 directly binds acidic lipid bilayers with a preference for highly curved bilayers. While this binding is nucleotide independent, LRRK2 can also deform low-curvature liposomes into narrow tubules in a guanylnucleotide-dependent but Adenosine 5′-triphosphate-independent way. Moreover, assembly of LRRK2 into scaffolds at the surface of lipid tubules can constrict them. We suggest that an interplay between the membrane remodeling and signaling properties of LRRK2 may be key to its physiological function. LRRK2, via its kinase activity, may achieve its signaling role at sites where membrane remodeling occurs.</p

YAP and β-catenin cooperate to drive H. pylori-induced gastric tumorigenesis

ABSTRACTH. pylori infection is the strongest known risk factor for gastric carcinoma. The activation of the yes-associated protein 1 (YAP) and β-catenin pathways has been associated with multiple tumor types. In this study, we investigated the crosstalk between the YAP and β-catenin pathways in H. pylori-associated gastric tumorigenesis. Immunohistochemical analysis of YAP and β-catenin expression was performed in human gastric cancer tissues. The small molecules Super-TDU and KYA1797K, pharmacological inhibitors of YAP and β-catenin, respectively, were used to investigate the role of these signaling pathways in H. pylori-induced gastric carcinogenesis in murine models of infection. The common downstream targets of YAP and β-catenin signaling were evaluated by RNA sequencing (RNA-seq). Western blot, immunofluorescence, luciferase, RT-PCR, immunoprecipitation, cell counting kit-8 (CCK8), EdU and spheroid assays were used. H. pylori infection promoted YAP and β-catenin nuclear accumulation and transcriptional activity in gastric epithelial cells and transgenic insulin–gastrin (INS-GAS) mice, whereas silencing of both YAP and β-catenin synergistically inhibited H. pylori-induced cell proliferation and expansion. In addition, YAP was found to directly interact with β-catenin and knockdown of YAP suppressed H. pylori-induced nuclear translocation of β-catenin. Moreover, downstream genes caudal-type homeobox 2 (CDX2), leucine-rich repeat containing G protein-coupled receptor 5 (LGR5) and RuvB like AAA ATPase 1 (RUVBL1) were shared by both YAP and β-catenin signaling. Furthermore, treatment with the YAP inhibitor Super-TDU or β-catenin inhibitor KYA1797A significantly alleviated gastric inflammation and epithelial DNA damage in H. pylori-infected mice. Finally, the elevation of gastric YAP was positively correlated with β-catenin expression in human gastric cancer tissues. These findings indicate that YAP and β-catenin synergistically promote H. pylori-induced gastric carcinogenesis via their physical interaction and reveal that CDX2, LGR5 and RUVBL1 are the downstream genes shared by both the YAP and β-catenin signaling pathways, and potentially contribute to H. pylori pathogenesis