Is I-Voting I-Llegal?

The Voting Rights Act was passed to prevent racial discrimination in all voting booths. Does the existence of a racial digital divide make Internet elections for public office merely a computer geek\u27s pipe dream? Or can i-voting withstand scrutiny under the current state of the law? This i-Brief will consider the current state of the law, and whether disproportionate benefits will be enough to stop this extension of technology dead in its tracks

Water refilling along vessels at initial stage of willow cuttage revealed by move contrast CT

Cuttage is a widely used technique for plant propagation, whose success relies on the refilling for water transport recovery. However, requirements for refilling characterization studies, including large penetration depth, fast temporal resolution and high spatial resolution, cannot be reached simultaneously via conventional imaging techniques. So far, the dynamic process of water refilling along the vessels at the initial stage of cuttage, as well as its characteristics, remains unclear. Hereby, we developed a move contrast X-ray microtomography method which achieves 3D dynamic non-destructive imaging of water refilling at the initial stage of willow branch cuttage, without the aid of any contrast agent. Experimental results indicate three primary refilling modalities in vessels: 1) the osmosis type, mainly manifested by the osmosis of tissue through the vessel wall into the cavity; 2) the linear type, revealed as the tissue permeates to a certain extent where the liquid column in the vessels is completely formed; and 3) an osmosis-linear mixed type refilling as an intermediate state. Further analysis also exhibits a “temporal-spatial relay” mode of refilling between adjacent vessels. Since the vessel length is quite limited, the cavitation and the relay refilling mode of vessels can be an important way to achieve long-distance water transport

Joint Probabilistic-Nyquist Pulse Shaping for an LDPC-Coded 8-PAM Signal in DWDM Data Center Communications

M-ary pulse-amplitude modulation (PAM) meets the requirements of data center communication because of its simplicity, but coarse entropy granularity cannot meet the dynamic bandwidth demands, and there is a large capacity gap between uniform formats and the Shannon limit. The dense wavelength division multiplexing (DWDM) system is widely used to increase the channel capacity, but low spectral efficiency of the intensity modulation/direct detection (IM/DD) solution restricts the throughput of the modern DWDM data center networks. Probabilistic shaping distribution is a good candidate to offer us a fine entropy granularity and efficiently reduce the gap to the Shannon limit, and Nyquist pulse shaping is widely used to increase the spectral efficiency. We aim toward the joint usage of probabilistic shaping and Nyquist pulse shaping with low-density parity-check (LDPC) coding to improve the bit error rate (BER) performance of 8-PAM signal transmission. We optimized the code rate of the LDPC code and compared different Nyquist pulse shaping parameters using simulations and experiments. We achieved a 0.43 dB gain using Nyquist pulse shaping, and a 1.1 dB gain using probabilistic shaping, while the joint use of probabilistic shaping and Nyquist pulse shaping achieved a 1.27 dB gain, which offers an excellent improvement without upgrading the transceivers.Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

Cdc42 is essential for the polarized movement and adhesion of human dental pulp stem cells

Objective: Stem cell-based tissue repair and regeneration require the regulation of cell migration and adhesion. As a regulator of cell polarization, Cdc42 (cell division control protein 42) plays a basic role at the initial stage of cell migration and adhesion. This study explores the effect of Cdc42 on the polarized migration and adhesion of hDPSCs (human dental pulp stem cells). Design: HDPSCs were isolated from extracted third molars and transfected with siRNA targeted against Cdc42. Scratch wound assays and transwell assays were performed to detect the migration of human dental pulp stem cells. Polarization assays were applied to explore the polarized movement of Golgi bodies and nuclei. Western blot was used to examine the expression of related proteins. Results: The expression of Cdc42 was knocked down by siRNA transfection, which inhibited the migration of hDPSCs in both the scratch wound assays and transwell assays. Meanwhile, the proportion of polarized hDPSCs during migration was also decreased, and the adhesion ability of hDPSCs was downregulated. Western blot demonstrated that these effects were dependent on FAK (focal adhesion kinase), β-catenin and GSK3β (Glycogen synthase kinase-3β). Conclusion Our study demonstrates that Cdc42 plays an essential role during the polarized movement and adhesion of hDPSCs

Development and application of oncolytic viruses as the nemesis of tumor cells

Viruses and tumors are two pathologies that negatively impact human health, but what occurs when a virus encounters a tumor? A global consensus among cancer patients suggests that surgical resection, chemotherapy, radiotherapy, and other methods are the primary means to combat cancer. However, with the innovation and development of biomedical technology, tumor biotherapy (immunotherapy, molecular targeted therapy, gene therapy, oncolytic virus therapy, etc.) has emerged as an alternative treatment for malignant tumors. Oncolytic viruses possess numerous anti-tumor properties, such as directly lysing tumor cells, activating anti-tumor immune responses, and improving the tumor microenvironment. Compared to traditional immunotherapy, oncolytic virus therapy offers advantages including high killing efficiency, precise targeting, and minimal side effects. Although oncolytic virus (OV) therapy was introduced as a novel approach to tumor treatment in the 19th century, its efficacy was suboptimal, limiting its widespread application. However, since the U.S. Food and Drug Administration (FDA) approved the first OV therapy drug, T-VEC, in 2015, interest in OV has grown significantly. In recent years, oncolytic virus therapy has shown increasingly promising application prospects and has become a major research focus in the field of cancer treatment. This article reviews the development, classification, and research progress of oncolytic viruses, as well as their mechanisms of action, therapeutic methods, and routes of administration

Distributional effects of vehicle tax in the framework of transportation externalities

Figure S2.The relationship between perivascular CD4 infiltration and 12 months follow-up DLCO (p = 0.134, r = −0.205). (PPT 43 kb

LATS kinase-mediated CTCF phosphorylation and selective loss of genomic binding.

Chromatin topological organization is instrumental in gene transcription. Gene-enhancer interactions are accommodated in the same CTCF-mediated insulated neighborhoods. However, it remains poorly understood whether and how the 3D genome architecture is dynamically restructured by external signals. Here, we report that LATS kinases phosphorylated CTCF in the zinc finger (ZF) linkers and disabled its DNA-binding activity. Cellular stress induced LATS nuclear translocation and CTCF ZF linker phosphorylation, and altered the landscape of CTCF genomic binding partly by dissociating it selectively from a small subset of its genomic binding sites. These sites were highly enriched for the boundaries of chromatin domains containing LATS signaling target genes. The stress-induced CTCF phosphorylation and locus-specific dissociation from DNA were LATS-dependent. Loss of CTCF binding disrupted local chromatin domains and down-regulated genes located within them. The study suggests that external signals may rapidly modulate the 3D genome by affecting CTCF genomic binding through ZF linker phosphorylation

Ultrafast Radiographic Imaging and Tracking: An overview of instruments, methods, data, and applications

Ultrafast radiographic imaging and tracking (U-RadIT) use state-of-the-art ionizing particle and light sources to experimentally study sub-nanosecond dynamic processes in physics, chemistry, biology, geology, materials science and other fields. These processes, fundamental to nuclear fusion energy, advanced manufacturing, green transportation and others, often involve one mole or more atoms, and thus are challenging to compute by using the first principles of quantum physics or other forward models. One of the central problems in U-RadIT is to optimize information yield through, e.g. high-luminosity X-ray and particle sources, efficient imaging and tracking detectors, novel methods to collect data, and large-bandwidth online and offline data processing, regulated by the underlying physics, statistics, and computing power. We review and highlight recent progress in: a.) Detectors; b.) U-RadIT modalities; c.) Data and algorithms; and d.) Applications. Hardware-centric approaches to U-RadIT optimization are constrained by detector material properties, low signal-to-noise ratio, high cost and long development cycles of critical hardware components such as ASICs. Interpretation of experimental data, including comparisons with forward models, is frequently hindered by sparse measurements, model and measurement uncertainties, and noise. Alternatively, U-RadIT makes increasing use of data science and machine learning algorithms, including experimental implementations of compressed sensing. Machine learning and artificial intelligence approaches, refined by physics and materials information, may also contribute significantly to data interpretation, uncertainty quantification and U-RadIT optimization.Comment: 51 pages, 31 figures; Overview of ultrafast radiographic imaging and tracking as a part of ULITIMA 2023 conference, Mar. 13-16,2023, Menlo Park, CA, US

Atomically resolved electrically active intragrain interfaces in perovskite semiconductors

Deciphering the atomic and electronic structures of interfaces is key to developing state-of-the-art perovskite semiconductors. However, conventional characterization techniques have limited previous studies mainly to grain-boundary interfaces, whereas the intragrain-interface microstructures and their electronic properties have been much less revealed. Herein using scanning transmission electron microscopy, we resolved the atomic-scale structural information on three prototypical intragrain interfaces, unraveling intriguing features clearly different from those from previous observations based on standalone films or nanomaterial samples. These intragrain interfaces include composition boundaries formed by heterogeneous ion distribution, stacking faults resulted from wrongly stacked crystal planes, and symmetrical twinning boundaries. The atomic-scale imaging of these intragrain interfaces enables us to build unequivocal models for the ab initio calculation of electronic properties. Our results suggest that these structure interfaces are generally electronically benign, whereas their dynamic interaction with point defects can still evoke detrimental effects. This work paves the way toward a more complete fundamental understanding of the microscopic structure–property–performance relationship in metal halide perovskites

Thyroid function and associated mood changes after COVID-19 vaccines in patients with Hashimoto thyroiditis

ContextSevere acute respiratory syndrome-coronavirus 2 (COVID-19) vaccines may incur changes in thyroid functions followed by mood changes, and patients with Hashimoto thyroiditis (HT) were suggested to bear a higher risk.ObjectivesWe primarily aim to find whether COVID-19 vaccination could induce potential subsequent thyroid function and mood changes. The secondary aim was to find inflammatory biomarkers associated with risk.MethodsThe retrospective, multi-center study recruited patients with HT receiving COVID-19–inactivated vaccines. C-reactive proteins (CRPs), thyroid-stimulating hormones (TSHs), and mood changes were studied before and after vaccination during a follow-up of a 6-month period. Independent association was investigated between incidence of mood state, thyroid functions, and inflammatory markers. Propensity score–matched comparisons between the vaccine and control groups were carried out to investigate the difference.ResultsFinal analysis included 2,765 patients with HT in the vaccine group and 1,288 patients in the control group. In the matched analysis, TSH increase and mood change incidence were both significantly higher in the vaccine group (11.9% versus 6.1% for TSH increase and 12.7% versus 8.4% for mood change incidence). An increase in CRP was associated with mood change (p< 0.01 by the Kaplan–Meier method) and severity (r = 0.75) after vaccination. Baseline CRP, TSH, and antibodies of thyroid peroxidase (anti-TPO) were found to predict incidence of mood changes.ConclusionCOVID-19 vaccination seemed to induce increased levels and incidence of TSH surge followed by mood changes in patients with HT. Higher levels of pre-vaccine serum TSH, CRP, and anti-TPO values were associated with higher incidence in the early post-vaccine phase