Sealing Clay Text Segmentation Based on Radon-Like Features and Adaptive Enhancement Filters

Text extraction is a key issue in sealing clay research. The traditional method based on rubbings increases the risk of sealing clay damage and is unfavorable to sealing clay protection. Therefore, using digital image of sealing clay, a new method for text segmentation based on Radon-like features and adaptive enhancement filters is proposed in this paper. First, adaptive enhancement LM filter bank is used to get the maximum energy image; second, the edge image of the maximum energy image is calculated; finally, Radon-like feature images are generated by combining maximum energy image and its edge image. The average image of Radon-like feature images is segmented by the image thresholding method. Compared with 2D Otsu, GA, and FastFCM, the experiment result shows that this method can perform better in terms of accuracy and completeness of the text

Synergistic effect of radiotherapy and dendritic-cell-based immunotherapy via miR-15b-5p regulation in a colon carcinoma mouse model

Purpose: To determine the combined effect of radiotherapy (RT) and dendritic cell (DC)-based immunotherapy on tumor regression, and the influence of miR-15b-5p on the sensitivity of the tumor to RT/DC. Methods: Colon carcinoma cell line (T-26) was injected subcutaneously into BALB/c mice to generate xenograft tumors. The animals were exposed to radiation therapy (RT) only or DC only, or RT plus DC (RT/DC). Tumor progression was measured and analyzed followed by quantification of miR-15b-5p using qPCR in each group 21 days after tumor inoculation. Overexpressed xenograft tumors were generated by transfecting MiR-15b-5p mimic to CT-26 cell lines and subcutaneously administered to mice. The mice were then subjected to RT/DC treatment and assessed for tumor growth, TUNEL staining, as well as mRNA and protein expressions of B-cell lymphoma-2 (BCL-2), caspase-3 (casp-3), and X-linked inhibitor of apoptosis protein (XIAP). Results: RT/DC combination treatment resulted in tumor regression and increased miR-15b-5p expression, when compared with other groups treated with RT and DC alone (p < 0.001). There was a marked reduction in tumor growth following RT/DC combination therapy in miR-15b-5p overexpressed xenograft tumor (p < 0.001). Moreover, miR-15b-5p/(RT/DC) treatment significantly increased cancer cell apoptosis. These results were substantiated by increased mRNA and protein expressions of casp-3, reduced BCL-2 and XIAP (p < 0.001). Conclusion: These findings demonstrate that RT/DC combination therapy increases miR-15b-5p levels in mice, and that miR-15b-5p upregulation enhances the sensitivity of tumors to RT/DC combination therapy. Therefore, the combination of RT and dendritic cell-based immunotherapy offers significant therapeutic advantage in controlling cancers, including metastatic tumors

Fractional Path Integrals and its degeneration to Dimensional Regularization

In this work we study particles propagate in a fractional path and use fractional derivatives to extend the dynamic dimension of Quantum Field Theory. we construct the Lagrangian of fractional scalar, vector and spinor fields to obtain their propagators by path integral. Then we compute the typical tree level and one loop diagrams which correspond to QED cases. The calculations show the dimension dependence of amplitudes. Additionally, in one loop calculation we obtain results which are consistent with dimensional regularization as the dimension approaches to the Standard Model value. Therefore, the fractional Path Integrals can be regarded as an equivalent theoretical representation for regularizing the divergence in the normal Quantum Field Theory. We also derive the equation of motion for scalar, vector and spinor particles propagate in fractal paths and discuss the corresponding gauge symmetry, where we find a special non-local gauge transformation.Comment: 17page

Establishing an experimental rat model of photodynamically-induced retinal vein occlusion using erythrosin B

<b>AIM:</b>To develop a reliable, reproducible rat model of retinal vein occlusion (RVO) with a novel photosensitizer (erythrosin B) and study the cellular responses in the retina.<b>METHODS</b>:Central and branch RVOs were created in adult male rats <i>via</i> photochemically-induced ischemia. Retinal changes were monitored<i> via</i> color fundus photography and fluorescein angiography at 1 and 3h, and 1, 4, 7, 14, and 21d after irradiation. Tissue slices were evaluated histopathologically. Retinal ganglion cell survival at different times after RVO induction was quantified by nuclear density count. Retinal thickness was also observed.<b>RESULTS</b>:For all rats in both the central and branch RVO groups, blood flow ceased immediately after laser irradiation and retinal edema was evident at one hour. The retinal detachment rate was 100% at 3h and developed into bullous retinal detachment within 24h. Retinal hemorrhages were not observed until 24h. Clearance of the occluded veins at 7d was observed by fluorescein angiography. Disease manifestation in the central RVO eyes was more severe than in the branch RVO group. A remarkable reduction in the ganglion cell count and retinal thickness was observed in the central RVO group by 21d, whereas moderate changes occurred in the branch RVO group.<b>CONCLUSION:</b> Rat RVO created by photochemically-induced ischemia using erythrosin B is a reproducible and reliable animal model for mimicking the key features of human RVO. However, considering the 100% rate of retinal detachment, this animal model is more suitable for studying RVO with chronic retinal detachment

Dexmedetomidine post-treatment attenuates cardiac ischaemia/reperfusion injury by inhibiting apoptosis through HIF-1α signalling.

Hypoxia-inducible factor 1α (HIF-1α) plays a critical role in the apoptotic process during cardiac ischaemia/reperfusion (I/R) injury. This study aimed to investigate whether post-treatment with dexmedetomidine (DEX) could protect against I/R-induced cardiac apoptosis in vivo and in vitro via regulating HIF-1α signalling pathway. Rat myocardial I/R was induced by occluding the left anterior descending artery for 30 minutes followed by 6-hours reperfusion, and cardiomyocyte hypoxia/reoxygenation (H/R) was induced by oxygen-glucose deprivation for 6 hours followed by 3-hours reoxygenation. Dexmedetomidine administration at the beginning of reperfusion or reoxygenation attenuated I/R-induced myocardial injury or H/R-induced cell death, alleviated mitochondrial dysfunction, reduced the number of apoptotic cardiomyocytes, inhibited the activation of HIF-1α and modulated the expressions of apoptosis-related proteins including BCL-2, BAX, BNIP3, cleaved caspase-3 and cleaved PARP. Conversely, the HIF-1α prolyl hydroxylase-2 inhibitor IOX2 partly blocked DEX-mediated cardioprotection both in vivo and in vitro. Mechanistically, DEX down-regulated HIF-1α expression at the post-transcriptional level and inhibited the transcriptional activation of the target gene BNIP3. Post-treatment with DEX protects against cardiac I/R injury in vivo and H/R injury in vitro. These effects are, at least in part, mediated via the inhibition of cell apoptosis by targeting HIF-1α signalling

Neonatal cardiac dysfunction and transcriptome changes caused by the absence of Celf1

The RNA binding protein Celf1 regulates alternative splicing in the nucleus and mRNA stability and translation in the cytoplasm. Celf1 is strongly down-regulated during mouse postnatal heart development. Its re-induction in adults induced severe heart failure and reversion to fetal splicing and gene expression patterns. However, the impact of Celf1 depletion on cardiac transcriptional and posttranscriptional dynamics in neonates has not been addressed. We found that homozygous Celf1 knock-out neonates exhibited cardiac dysfunction not observed in older homozygous animals, although homozygous mice are smaller than wild type littermates throughout development. RNA-sequencing of mRNA from homozygous neonatal hearts identified a network of cell cycle genes significantly up-regulated and down-regulation of ion transport and circadian genes. Cell cycle genes are enriched for Celf1 binding sites supporting a regulatory role in mRNA stability of these transcripts. We also identified a cardiac splicing network coordinated by Celf1 depletion. Target events contain multiple Celf1 binding sites and enrichment in GU-rich motifs. Identification of direct Celf1 targets will advance our knowledge in the mechanisms behind developmental networks regulated by Celf1 and diseases where Celf1 is mis-regulated

Sealing Clay Text Segmentation Based on Radon-Like Features and Adaptive Enhancement Filters

Text extraction is a key issue in sealing clay research. The traditional method based on rubbings increases the risk of sealing clay damage and is unfavorable to sealing clay protection. Therefore, using digital image of sealing clay, a new method for text segmentation based on Radon-like features and adaptive enhancement filters is proposed in this paper. First, adaptive enhancement LM filter bank is used to get the maximum energy image; second, the edge image of the maximum energy image is calculated; finally, Radon-like feature images are generated by combining maximum energy image and its edge image. The average image of Radon-like feature images is segmented by the image thresholding method. Compared with 2D Otsu, GA, and FastFCM, the experiment result shows that this method can perform better in terms of accuracy and completeness of the text