Roles of TGFβ and FGF signals during growth and differentiation of mouse lens epithelial cell in vitro.

Transforming growth factor β (TGFβ) and fibroblast growth factor (FGF) signaling pathways play important roles in the proliferation and differentiation of lens epithelial cells (LECs) during development. Low dosage bFGF promotes cell proliferation while high dosage induces differentiation. TGFβ signaling regulates LEC proliferation and differentiation as well, but also promotes epithelial-mesenchymal transitions that lead to cataracts. Thus far, it has been difficult to recapitulate the features of germinative LECs in vitro. Here, we have established a LEC culture protocol that uses SB431542 (SB) compound to inhibit TGFβ/Smad activation, and found that SB treatment promoted mouse LEC proliferation, maintained LECs' morphology and distinct markers including N-cadherin, c-Maf, Prox1, and αA-, αB-, and β-crystallins. In contrast, low-dosage bFGF was unable to sustain those markers and, combined with SB, altered LECs' morphology and β-crystallin expression. We further found that Matrigel substrate coatings greatly increased cell proliferation and uniquely affected β-crystallin expression. Cultured LECs retained the ability to differentiate into γ-crystallin-positive lentoids by high-dosage bFGF treatment. Thus, a suppression of TGFβ/Smad signaling in vitro is critical to maintaining characteristic features of mouse LECs, especially expression of the key transcription factors c-Maf and Prox1

Bis(1H-benzotriazole-4-sulfonato-κ2 N 3,O)(2,2′-bipyridyl-κ2 N,N′)cadmium

In the title complex, [Cd(C6H4N3O3S)2(C10H8N2)], the Cd2+ cation is located on a twofold rotation axis and is coordinated by two N and two O atoms from two symmetry-related benzotriazole-4-sulfonate anions and two N atoms from a 2,2-bipyridyl ligand, displaying a distorted CdN4O2 octa­hedral geometry. The crystal structure is stabilized by N—H⋯O and C—H⋯O hydrogen-bonding inter­actions

Bis(1H-benzotriazole-7-sulfonato-κO)bis­(1,10-phenanthroline-κ2 N,N′)cadmium dihydrate

In the title complex, [Cd(C6H4N3O3S)2(C12H8N2)2]·2H2O, the Cd2+ cation is located on an inversion center and is coordinated by four N atoms from two symmetry-related 1,10-phenanthroline ligands and two sulfonate O atoms from two benzotriazole-7-sulfonate anions, displaying a distorted CdN4O2 octa­hedral geometry. In the crystal, O—H⋯N, O—H⋯O, N—H⋯O, C—H⋯N and C—H⋯O hydrogen bonds occur. The lattice water mol­ecules and sulfonate O atoms as donor or acceptor atoms play important roles in the formation of these inter­actions

Geometric bionics: Lotus effect helps polystyrene nanotube films get good blood compatibility

Various biomaterials have been widely used for manufacturing biomedical applications including artificial organs, medical devices and disposable clinical apparatus, such as vascular prostheses, blood pumps, artificial kidney, artificial hearts, dialyzers and plasma separators, which could be used in contact with blood^1^. However, the research tasks of improving hemocompatibility of biomaterials have been carrying out with the development of biomedical requirements^2^. Since the interactions that lead to surface-induced thrombosis occurring at the blood-biomaterial interface become a reason of familiar current complications with grafts therapy, improvement of the blood compatibility of artificial polymer surfaces is, therefore a major issue in biomaterials science^3^. After decades of focused research, various approaches of modifying biomaterial surfaces through chemical or biochemical methods to improve their hemocompatibility were obtained^1^. In this article, we report that polystyrene nanotube films with morphology similar to the papilla on lotus leaf can be used as blood-contacted biomaterials by virtue of Lotus effect^4^. Clearly, this idea, resulting from geometric bionics that mimicking the structure design of lotus leaf, is very novel technique for preparation of hemocompatible biomaterials

Study on Complex Products Job-shop Scheduling System Based on MES

Various random disturbances that happen in the process of complex products (ship) production cannot feedback timely, and the plans of job-shop operation are too rough to instruct job-shop production. The purpose of this study is to solve the problems above, help the course of complex products (ship) job-shop production runs orderly and efficiently, and improve the job-shop on-site management level. In this paper, we proposed complex products job-shop scheduling system based on MES. The system adopts six-level, refined plan and scheduling mechanism. Its key part is the job-shop scheduling model with man-machine coordinated mechanism. What’s more, an improved Genetic Algorithm based on TOC is proposed to make the optimized algorithm module of the system more scientific and effective. Key words: Complex Products; MES; Job-shop Scheduling System; Man-machine Coordinated Mechanism; Genetic Algorithm Résumé: Diverses perturbations qui se produisent au hasard dans le processus de production des produits complexes (navire) ne peuvent pas être apperçues en temps opportun, et les plans de l'opération des ateliers sont trop approximatifs pour guider la production. Le but de cette étude consiste à résoudre les problèmes ci-dessus, rendre le cours de la production des produits complexes (ship) dans les ateliers ordonné et efficace et améliorer le niveau de gestion sur place. Dans cet article, nous avons proposé le système de planning des ateliers de produits complexes basé sur MES. Le système contient six niveaux de programmation raffinée et de mécanismes de planning. Son élément essentiel est le modèle de programmation avec les mécanismes de coordination homme-machine. De plus, un algorithme génétique amélioré basé sur TOC est également proposé de rendre le module de l'algorithme du système optimisé plus scientifique et efficace. Mots-clés: produits complexes; MES; système de planning des ateliers; mécanismes de coordination homme-machine; algorithme génétique 摘要：針對目前複雜產品（船舶）生產過程中所出現的各種隨機擾動不能得到及時回饋，以及車間作業計畫粗略難以直接指導車間生產的現狀，本文提出基於 MES的複雜產品車間作業調度系統。該系統採用逐層細化的五級計畫與調度機制，以人機協同的車間作業調度模型為核心，通過改進基於 TOC的遺傳演算法，使系統的演算法模組優化性能更具有科學性和操作性。該系統有助於實現複雜產品（船舶）車間生產過程的有序運作，從而提高生產車間的現場管理水準。 關鍵字：複雜產品； MES；車間作業調度；人機協同；遺傳演算

The correlation between the severity of radiotherapy-induced glossitis and endothelial cell injury in local tissues in a rat model

Objectives: To explore the correlation between the severity of radiotherapy-induced glossitis (RTG) and endothelial cell injury in local tissues in a rat model. Study Design: The RTG animal model was designed and used by our team. The Oral mucositis index(OMI) was documented daily. Immunohistochemistry (IHC) Staining of CD34 was utilized to identify endothelial cells in the RTG tissues. Apoptosis of endothelial cells in local lesions due to RTG was detected by the TUNEL assay. The dynamic relationship between the OMI and apoptotic endothelial cells was statistically analyzed by time. Results and Conclusions: The injury and apoptosis of endothelial cells were observed 3 day post-irradiation. The vascular lumens of the post-irradiation tongue lesions were irregular; thrombosis formation in the center of the lumens, unsmooth lumen walls and vasodilated vessels were observed. Also, endothelial cells detached from the basal membrane and were found in the lumens. The percentages (%) of apoptotic endothelial cells were 78.3±0.31 (5 day); 89.3±0.83 (8 day); 83.5±0.41 (14 day); 69.3±0.57 (21 day); and 47.3±0.59 (28 day). The OMI was correlated with the percentage of apoptotic endothelial cells (R=0.67, P=0.034). Summary, endothelial cell injury was correlated with the pathogenic condition of RTG. © Medicina Oral S. L

Research on BOM Mapping transformation for Ship Construction Process

Abstract: Due to the problem about the core departmental "information isolated island" with BOM, various stages of ship product design section are convergence off and impeded the construction of agile ship the product. In this paper, first, establish the various stages of the ship product BOM structure model, based on a comparison of the structural model analysis, design a more practical conversion method, and then describe the conversion process about the ship product life cycle BOM view in order to quickly build a variety of BOM view. Keywords: BOM; BOM tectonic model; BOM view of conversio

A simple and efficient method for isolating polymorphic microsatellites from cDNA

<p>Abstract</p> <p>Background</p> <p>Microsatellites in cDNA are useful as molecular markers because they represent transcribed genes and can be used as anchor markers for linkage and comparative mapping, as well as for studying genome evolution. Microsatellites in cDNA can be detected in existing ESTs by data mining. However, in most fish species, no ESTs are available or the number of ESTs is limited, although fishes represent half of the vertebrates on the earth. We developed a simple and efficient method for isolation of microsatellites from cDNA in fish.</p> <p>Results</p> <p>The method included normalization of 150 ng cDNA using 0.5 U duplex-specific nuclease (DSN) at 65°C for 30 min, enrichment of microsatellites using biotinylated oligonucleotides and magnetic field, and directional cloning of cDNA into a vector. We tested this method to enrich CA- and GA-microsatellites from cDNA of Asian seabass, and demonstrated that enrichment of microsatellites from normalized cDNA could increased the efficiency of microsatellite isolation over 30 times as compared to direct sequencing of clones from cDNA libraries. One hundred and thirty-nine (36.2%) out of 384 clones from normalized cDNA contained microsatellites. Unique microsatellite sequences accounted for 23.6% (91/384) of sequenced clones. Sixty microsatellites isolated from cDNA were characterized, and 41 were polymorphic. The average allele number of the 41 microsatellites was 4.85 ± 0.54, while the expected heterozygosity was 0.56 ± 0.03. All the isolated microsatellites inherited in a Mendelian pattern.</p> <p>Conclusion</p> <p>Normalization of cDNA substantially increased the efficiency of enrichment of microsatellites from cDNA. The described method for isolation of microsatellites from cDNA has the potential to be applied to a wide range of fish species. The microsatellites isolated from cDNA could be useful for linkage and comparative mapping, as well as for studying genome evolution.</p