Validation of Expression Patterns for Nine miRNAs in 204 Lymph-Node Negative Breast Cancers

INTRODUCTION: Although lymph node negative (LN-) breast cancer patients have a good 10-years survival (∼85%), most of them still receive adjuvant therapy, while only some benefit from this. More accurate prognostication of LN- breast cancer patient may reduce over- and under-treatment. Until now proliferation is the strongest prognostic factor for LN- breast cancer patients. The small molecule microRNA (miRNA) has opened a new window for prognostic markers, therapeutic targets and/or therapeutic components. Previously it has been shown that miR-18a/b, miR-25, miR-29c and miR-106b correlate to high proliferation. METHODS: The current study validates nine miRNAs (miR-18a/b miR-25, miR-29c, miR-106b, miR375, miR-424, miR-505 and let-7b) significantly correlated with established prognostic breast cancer biomarkers. Total RNA was isolated from 204 formaldehyde-fixed paraffin embedded (FFPE) LN- breast cancers and analyzed with quantitative real-time Polymerase Chain Reaction (qPCR). Independent T-test was used to detect significant correlation between miRNA expression level and the different clinicopathological features for breast cancer. RESULTS: Strong and significant associations were observed for high expression of miR-18a/b, miR-106b, miR-25 and miR-505 to high proliferation, oestrogen receptor negativity and cytokeratin 5/6 positivity. High expression of let-7b, miR-29c and miR-375 was detected in more differentiated tumours. Kaplan-Meier survival analysis showed that patients with high miR-106b expression had an 81% survival rate vs. 95% (P = 0.004) for patients with low expression. CONCLUSION: High expression of miR-18a/b are strongly associated with basal-like breast cancer features, while miR-106b can identify a group with higher risk for developing distant metastases in the subgroup of Her2 negatives. Furthermore miR-106b can identify a group of patients with 100% survival within the otherwise considered high risk group of patients with high proliferation. Using miR-106b as a biomarker in conjunction to mitotic activity index could thereby possibly save 18% of the patients with high proliferation from overtreatment

Carcass and parts yield of broilers reared under a semi-extensive system

An experiment was carried out to evaluate carcass traits of male and female broilers of two genetic strains (Paraíso Pedrês and Pescoço Pelado), slaughtered at 65, 75, 85 and 95 days of age, and reared under a semi-extensive production system. The following parameters were evaluated: body weight at slaughter (BWs), carcass weight (CW), carcass yield (CY), parts yield, including breast, drumstick, thighs, back, neck, feet, wings, abdominal fat pad (AF), and giblets (gizzard, heart, liver). Paraíso Pedrês males presented higher BWs and CW. Paraíso Pedrês females had higher breast yield. Thighs and drumstick yields were higher in Pescoço Pelado males. Back yield was similar at 85 and 95 days of age, wing yield declined with age. AF yield was higher in Paraíso Pedrês and in females. GY was affected by sex and slaughter age. The results of this work lead to the conclusion that Paraíso Pedrês males reach higher live and carcass weights at 85 and 95 days of age, and that breast yield is higher in this strain. On the other hand, Pescoço Pelado males present higher drumstick and thighs yields. Therefore, the choice of genetic strain, sex and slaughter weight will determine carcasses with different parts yield

Enfermagem obstétrica: contribuições às metas dos Objetivos de Desenvolvimento do Milênio

Objetivo: Caracterizar e analisar a assistência ao parto e ao nascimento realizada por Residentes em Enfermagem Obstétrica.Método: Estudo quantitativo e retrospectivo de 189 partos normais assistidos por Residentes em Enfermagem Obstétrica, no período de julho de 2013 a junho de 2014, em uma maternidade localizada no interior do Rio Grande do Sul. A coleta dos dados deu-se atravésde levantamento de informações dos prontuários clínicos e, para a análise, utilizou-se a estatística descritiva.Resultados: Constatou-se o amplo uso de métodos não invasivos e não farmacológicos de alívio da dor e a liberdade de posição durante o trabalho de parto. Destaca-se que 55,6% das mulheres não foram submetidas a nenhuma intervenção obstétrica.Conclusões: Foi possível identificar que o Programa de Residência em Enfermagem possibilita a redução de intervenções obstétricas, refletindo diretamente na melhoria da saúde materna.Palavras-chave: Obstetrícia. Enfermeiras obstétricas. Parto. Parto humanizado. Saúde materno-infantil. Internato não médico. Objetivos de Desenvolvimento do Milênio