New structural insights into the multifunctional influenza A matrix protein 1

Influenza A virus matrix protein 1 (M1) is the most abundant protein within virions and functions at multiple steps of the virus life cycle, including nuclear RNA export, virus particle assembly, and virus disassembly. Two recent publications have presented the first structures of full-length M1 and show that it assembles filaments in vitro via an interface between the N- and C-terminal domains of adjacent monomers. These filaments were found to be similar to those that form the endoskeleton of assembled virions. The structures provide a molecular basis to understand the functions of M1 during the virus life cycle. Here, we compare and discuss the two structures, and explore their implications for the mechanisms by which the multifunctional M1 protein can mediate virus assembly, interact with viral ribonucleoproteins and act during infection of a new cell

Molecular and mass spectroscopic analysis of isotopically labeled organic residues

Experimental studies aimed at understanding the evolution of complex organic molecules on interstellar grains were performed. The photolysis of frozen gas mixtures of various compositions containing H2O, CO, NH3, and CH4 was studied. These species were chosen because of their astrophysical importance as deducted from observational as well as theoretical studies of ice mantles on interstellar grains. These ultraviolet photolyzed ices were warmed up in order to produce refractory organic molecules like the ones formed in molecular clouds when the icy mantles are being irradiated and warmed up either by a nearby stellar source or impulsive heating. The laboratory studies give estimates of the efficiency of production of such organic material under interstellar conditions. It is shown that the gradual carbonization of organic mantles in the diffuse cloud phase leads to higher and higher visual absorptivity - yellow residues become brown in the laboratory. The obtained results can be applied to explaining the organic components of comets and their relevance to the origin of life

The spin temperature of high-redshift damped Lyman-α\alpha systems

We report results from a programme aimed at investigating the temperature of neutral gas in high-redshift damped Lyman-$\alpha$ absorbers (DLAs). This involved (1) HI 21cm absorption studies of a large DLA sample, (2) VLBI studies to measure the low-frequency quasar core fractions, and (3) optical/ultraviolet spectroscopy to determine DLA metallicities and velocity widths. Including literature data, our sample consists of 37 DLAs with estimates of the spin temperature $T_s$ and the covering factor. We find a strong $4\sigma$) difference between the $T_s$ distributions in high-z (z>2.4) and low-z (z<2.4) DLA samples. The high-z sample contains more systems with high $T_s$ values, $\gtrsim 1000$ K. The $T_s$ distributions in DLAs and the Galaxy are also clearly (~$6\sigma$) different, with more high-$T_s$ sightlines in DLAs than in the Milky Way. The high $T_s$ values in the high-z DLAs of our sample arise due to low fractions of the cold neutral medium. For 29 DLAs with metallicity [Z/H] estimates, we confirm the presence of an anti-correlation between $T_s$ and [Z/H], at $3.5\sigma$ significance via a non-parametric Kendall-tau test. This result was obtained with the assumption that the DLA covering factor is equal to the core fraction. Monte Carlo simulations show that the significance of the result is only marginally decreased if the covering factor and the core fraction are uncorrelated, or if there is a random error in the inferred covering factor. We also find evidence for redshift evolution in DLA $T_s$ values even for the z>1 sub-sample. Since z>1 DLAs have angular diameter distances comparable to or larger than those of the background quasars, they have similar efficiency in covering the quasars. Low covering factors in high-z DLAs thus cannot account for the observed redshift evolution in spin temperatures. (Abstract abridged.)Comment: 37 pages, 22 figures. Accepted for publication in Monthly Notices of the Royal Astronomical Societ

Timesaving Double-Grid Method for Real-Space Electronic-Structure Calculations

We present a simple and efficient technique in ab initio electronic-structure calculation utilizing real-space double-grid with a high density of grid points in the vicinity of nuclei. This technique promises to greatly reduce the overhead for performing the integrals that involves non-local parts of pseudopotentials, with keeping a high degree of accuracy. Our procedure gives rise to no Pulay forces, unlike other real-space methods using adaptive coordinates. Moreover, we demonstrate the potential power of the method by calculating several properties of atoms and molecules.Comment: 4 pages, 5 figure

Large Scale Electronic Structure Calculations with Multigrid Acceleration

We have developed a set of techniques for performing large scale ab initio calculations using multigrid accelerations and a real-space grid as a basis. The multigrid methods permit efficient calculations on ill-conditioned systems with long length scales or high energy cutoffs. The technique has been applied to systems containing up to 100 atoms, including a highly elongated diamond cell, an isolated C$_{60}$ molecule, and a 32-atom cell of GaN with the Ga d-states in valence. The method is well suited for implementation on both vector and massively parallel architectures.Comment: 4 pages, 1 postscript figur

Photometric Monitoring of Open Clusters I. The Survey

Open clusters, which have age, abundance, and extinction information from studies of main-sequence turn off stars, are the ideal location in which to determine the mass-luminosity-radius relation for low-mass stars. We have undertaken a photometric monitoring survey of open clusters in the Galaxy designed to detect low-mass eclipsing binary systems through variations in their relative light curves. Our aim is to provide an improved calibration of the mass-luminosity-radius relation for low-mass stars and brown dwarfs, to test stellar structure and evolution models, and to help quantify the contribution of low-mass stars to the global mass census in the Galaxy. In this paper we present our survey, describing the data and outlining the analysis techniques. We study six nearby open clusters, with a range of ages from $\sim 0.2$ to 4 Gyr and metallicities from approximately solar to -0.2dex. We monitor a field-of-view of > 1 square degree per target cluster, well beyond the characteristic cluster radius, over timescales of hours, days, and months with a sampling rate optimised for the detection of eclipsing binaries with periods of hours to days. Our survey depth is designed to detect eclipse events in a binary with a primary star of \lesssim 0.3~M_{\sun}. Our data have a photometric precision of $\sim 3$ mmag at $I\approx 16$.Comment: 50 pages, 18 figures, accepted for publication in A

Subregional DXA-derived vertebral bone mineral measures are stronger predictors of failure load in specimens with lower areal bone mineral density, compared to those with higher areal bone mineral density

Measurement of areal bone mineral density (aBMD) in intravertebral subregions may increase the diagnostic sensitivity of dual-energy X-ray absorptiometry (DXA)-derived parameters for vertebral fragility. This study investigated whether DXA-derived bone parameters in vertebral subregions were better predictors of vertebral bone strength in specimens with low aBMD, compared to those with higher aBMD. Twenty-five lumbar vertebrae (15 embalmed and 10 fresh-frozen) were scanned with posteroanterior- (PA) and lateral-projection DXA, and then mechanically tested in compression to ultimate failure. Whole-vertebral aBMD and bone mineral content (BMC) were measured from the PA- and lateral-projection scans and within 6 intravertebral subregions. Multivariate regression was used to predict ultimate failure load by BMC, adjusted for vertebral size and specimen fixation status across the whole specimen set, and when subgrouped into specimens with low aBMD and high aBMD. Adjusted BMC explained a substantial proportion of variance in ultimate vertebral load, when measured over the whole vertebral area in lateral projection (adjusted R2 0.84) and across the six subregions (ROIs 2–7) (adjusted R2 range 0.58–0.78). The association between adjusted BMC, either measured subregionally or across the whole vertebral area, and vertebral failure load, was increased for the subgroup of specimens with identified ‘low aBMD’, compared to those with ‘high aBMD’, particularly in the anterior subregion where the adjusted R2 differed by 0.44. The relative contribution of BMC measured in vertebral subregions to ultimate failure load is greater among specimens with lower aBMD, compared to those with higher aBMD, particularly in the anterior subregion of the vertebral body

Roles of tetrahydrobiopterin in promoting tumor angiogenesis.

Nitric oxide (NO), which is derived from endothelial NO synthase (eNOS), provides crucial signals for angiogenesis in the tumor microenvironment. Tetrahydrobiopterin (BH4) is an absolute requirement for eNOS activity. In this study, we investigated whether this activation is both maintained by a wild-type Ras/phosphatidylinositol 3-kinase (PI3K)/Akt-positive feedback loop in endothelial cells and affects tumor angiogenesis. We found that supplementation of BH4 (via the pterin salvage pathway with Sep) increased Akt/eNOS phosphorylation in both human eNOS-transfected COS-7 cells and endothelial cells concomitant with increases in NO production, cell proliferation, migration, and tube formation. This augmentation was abrogated by a PI3K inhibitor. Sepiapterin (Sep) also increased GTP-bound wild-type Ras and PI3K/Akt/eNOS activation, which was prevented by the eNOS inhibitor, Nω-Nitro-L-arginine methyl ester (L-NAME). Furthermore, expression of GTP cyclohydrolase I (the rate-limiting enzyme in de novo BH4 synthesis) under doxycycline control potentiated in vivo tumorigenesis, tumor cell proliferation, as well as angiogenesis. Conversely, both switching off GTP cyclohydrolase I expression as well as inhibiting its enzymatic activity significantly decreased eNOS expression and tumor vascularization. This study demonstrates an important role for BH4 synthesis in angiogenesis by the activation of eNOS for NO production, which is maintained by a PI3K/Akt-positive feedback loop through effects on wild-type Ras in endothelial cells. Our findings suggest that BH4 synthesis may be a rational target for antiangiogenesis therapy for tumors

Characterization Of Epoxy-Coated Oxide Films Using Acoustic Microscopy

An adhesive joint consisting of aluminum adherends bonded with an epoxy adhesive is composed of three main layers. The adherends are usually a few millimeters thick with a layer of epoxy adhesive between one and three hundred microns thick between them. The surfaces of the adherends are typically pre-treated to produce a thin film of porous aluminum oxide, which has a honeycomb-like structure. The epoxy adhesive may then penetrate into these honeycomb cells or pores. The resulting layer between the adhesive and adherend is therefore a micro-composite and it is typically of the order of one micron in thickness. The use of the surface pre-treatment is a major factor in increasing the durability of the adhesive joint when it is exposed to water. Additionally, joints which have been in use for some time, especially ones which have been subject to environmental attack, usually experience a failure along the plane of this film. Therefore, characterization of this epoxy/oxide interlayer is very important in understanding adhesive joints and how they are affected by environmental factors. Unfortunately, not much is known about their mechanical properties

Na incorporation into Cu(In,Ga)Se2 thin-film solar cell absorbers deposited on polyimide: Impact on the chemical and electronic surface structure

The following article appeared in Journal of Applied Physics 111.3 (2012): 034903 and may be found at http://scitation.aip.org/content/aip/journal/jap/111/3/10.1063/1.3679604Na has deliberately been incorporated into Cu(In,Ga)Se2 (CIGSe) chalcopyrite thin-film solar cell absorbers deposited on Mo-coated polyimide flexible substrates by adding differently thick layers of NaF in-between CIGSe absorber and Mo back contact. The impact of Na on the chemical and electronic surface structure of CIGSe absorbers with various Cu-contents deposited at comparatively low temperature (420 C) has been studied using x-ray photoelectron and x-ray excited Auger electron spectroscopy. We observe a higher Na surface content for the Cu-richer CIGSe samples and can distinguish between two different chemical Na environments, best described as selenide-like and oxidized Na species, respectively. Furthermore, we find a Cu-poor surface composition of the CIGSe samples independent of Na content and - for very high Na contents - indications for the formation of a (Cu,Na)-(In,Ga)-Se like compound. With increasing Na surface content, also a shift of the photoemission lines to lower binding energies could be identified, which we interpret as a reduction of the downward band bending toward the CIGSe surface explained by the Na-induced elimination of In Cu defects.X.S., R.F., D.G., R.G.W., and M.B. are grateful to the Helmholtz-Association for financial support (VH-NG-423). R.F. also acknowledges the support by the German Academic Exchange Agency (DAAD; 331 4 04 002)