The validity of a teacher administered rating chart for talks at the secondary level.

Thesis (Ed.M.)--Boston Universit

THE OXYGEN EQUILIBRIUM OF CYSTINE-TREATED HUMAN HEMOGLOBIN WITHOUT FREE SULFHYDRYL GROUPS.

n/

Provincetown, yesterday and today.

Thesis (Ed.M.)--Boston Universit

第7章 平成22年度～24年度特別研究 : 「地域歴史遺産保全活用教育研究を基軸とした地域歴史文化育成支援拠点の整備」事業

textabstractThe Mre11 complex (Mre11-Rad50-Nbs1 or MRN) binds double-strand breaks where it interacts with CtIP/Ctp1/Sae2 and ATM/Tel1 to preserve genome stability through its functions in homology-directed repair, checkpoint signaling and telomere maintenance. Here, we combine biochemical, structural and in vivo functional studies to uncover key properties of Mre11-W243R, a mutation identified in two pediatric cancer patients with enhanced ataxia telangiectasia-like disorder. Purified human Mre11-W243R retains nuclease and DNA binding activities in vitro. X-ray crystallography of Pyrococcus furiosus Mre11 indicates that an analogous mutation leaves the overall Mre11 threedimensional structure and nuclease sites intact but disorders surface loops expected to regulate DNA and Rad50 interactions. The equivalent W248R allele in fission yeast allows Mre11 to form an MRN complex that efficiently binds double-strand breaks, activates Tel1/ATM and maintains telomeres; yet, it causes hypersensitivity to ionizing radiation and collapsed replication forks, increased Rad52 foci, defective Chk1 signaling and meiotic failure. W248R differs from other ataxia telangiectasia-like disorder analog alleles by the reduced stability of its interaction with Rad50 in cell lysates. Collective results suggest a separation-of-function mutation that disturbs interactions amongst the MRN subunits and Ctp1 required for DNA end processing in vivo but maintains interactions sufficient for Tel1/ATM checkpoint and telomere maintenance functions

A Non-singular Theory of Gravity

We present a geometrical gravitational theory which reduces to Einstein's theory for weak gravitational potentials and which has a singularity-free analog of the Schwarzschild metric.Comment: 8 pages, RevTeX, 3 figures included in compressed form, UTPT-94-0

STUDIES ON THE OXIDATION-REDUCTION POTENTIALS OF HEME PROTEINS. II. CARBOXYPEPTIDASE DIGESTS OF HUMAN HEMOGLOBIN.

n/

Interview with Claude Wyman, Harry Moos, and Laveta Green

An interview with Claude Wyman, Harry Moos, and Laveta Green containing various songs and stories. The beginning of this recording is missing. 00:00:00 - Claude Wyman, shoeing horses 00:04:26 - Farming near Radium, KS in 1916 00:10:04 - Marriage 00:11:08 - Breaking sod 00:13:56 - Jack rabbits 00:16:39 - Childhood games 00:20:13 - Making railroad ties 00:21:24 - Harry Moos, introduction to French fairy tales 00:22:32 - Story, The Spotted Bull 00:25:29 - Story, The White Cat 00:31:39 - Story, The Good Man of Butter or Old Marguerite 00:37:51 - Story, The Billy Goats Gruff and final remarks 00:38:28 - Laveta P. Green, introduction 00:38:53 - Song, To Make Her Old Man Go Blind 00:40:02 - Song, The Cat Came Back 00:41:11 - Song, Whistlin\u27 Rufus 00:41:56 - Song, The Crickets Say 00:42:23 - Song, My Kitty 00:43:00 - Song, Little Bluebird In The Tree 00:43:54 - Song, Star Light, Star Bright 00:44:25 - Song, Poor Babes in the Wood 00:45:50 - Song, Oklahoma 00:47:50 - Song, Hand Me Down My Walkin\u27 Cane 00:50:09 - Song, It Ain\u27t Gonna Rain No More 00:50:59 - Song, When I Was Single 00:50:21 - Song, Prisoner\u27s Song 00:53:43 - Song, A Gay Caballero 00:55:15 - Song, My Darlin\u27 Clementine 00:57:23 - Song, Charlie 00:58:23 - Song, Everybody\u27s Happy 00:59:45 - Song, I Love Coffee 01:00:09 - Song, I Wish I Had a Nickel 01:00:24 - Song, Old Mrs. Shady 01:00:45 - Song, The Horses Running \u27Round 01:01:07 - Song, She\u27s a Darling 01:01:27 - Song, Little Willie 01:02:06 - Song, Grasshopper Settin On A Sweet potato Vine 01:02:28 - Song, Eighteen Miles From Wiggletown to Waggletown 01:02:50 - Song, A Young Lady From Nantucket 01:03:14 - Song, Green Gravel 01:03:39 - Song, Needles-eye 01:04:04 - Song, Somebody\u27s Mad 01:04:19 - Song, Baby-o Victoria Volga-German Wedding Dance 01:04:32 - Song, Braut Dusch 01:07:21 - Song, Schottische: Put Your Little Foot 01:10:10 - Song, Polkahttps://scholars.fhsu.edu/sackett/1041/thumbnail.jp

Portfolio Vol. IV N 4

Wager, Dick. Collective Bargaining. Prose. 3-5. Raymond, Toby. Lochinvar Rides Again. Poetry. 6. Wyman, John. Lily of the Alley. Prose. 8-9. Benson, Virginia. Lost Cause. Prose. 10. Collins, Peg. The Curtain Falls. Prose. 11. Dorsey, Ann. Jack. Picture. 12. Sulke, Jim. Old Pete. Picture. 12. Myers, Sue. Jerry. Picture. 12. Rosenthal, Joan. Canadian Farmhouse. Picture. 13. Deeds, Ed. Mirror Lake. Picture. 13. Peters, Doris. Trees. Picture. 13. Seagrave, Leslie. Chinese Coins. Poetry. 14. White, Molly. Thoughts in the Rain. Poetry. 14. Smith, Duke. Keeping the Records Straight. Prose. 15. Stocker, Ed. Joe. Prose. 16-17. Saunders, John. Seven Year\u27s Luck. Prose. 19-22

Human Rad51 filaments on double- and single-stranded DNA: correlating regular and irregular forms with recombination function

Recombinase proteins assembled into helical filaments on DNA are believed to be the catalytic core of homologous recombination. The assembly, disassembly and dynamic rearrangements of this structure must drive the DNA strand exchange reactions of homologous recombination. The sensitivity of eukaryotic recombinase activity to reaction conditions in vitro suggests that the status of bound nucleotide cofactors is important for function and possibly for filament structure. We analyzed nucleoprotein filaments formed by the human recombinase Rad51 in a variety of conditions on double-stranded and single-stranded DNA by scanning force microscopy. Regular filaments with extended double-stranded DNA correlated with active in vitro recombination, possibly due to stabilizing the DNA products of these assays. Though filaments formed readily on single-stranded DNA, they were very rarely regular structures. The irregular structure of filaments on single-stranded DNA suggests that Rad51 monomers are dynamic in filaments and that regular filaments are transient. Indeed, single molecule force spectroscopy of Rad51 filament assembly and disassembly in magnetic tweezers revealed protein association and disassociation from many points along the DNA, with kinetics different from those of RecA. The dynamic rearrangements of proteins and DNA within Rad51 nucleoprotein filaments could be key events driving strand exchange in homologous recombination

DNA bending by photolyase in specific and non-specific complexes studied by atomic force microscopy

Specific and non-specific complexes of DNA and photolyase are visualised by atomic force microscopy. As a substrate for photolyase a 1150 bp DNA restriction fragment was UV-irradiated to produce damaged sites at random positions. Comparison with a 735 bp undamaged DNA fragment made it possible to separate populations of specific and non-specific photolyase complexes on the 1150 bp fragment, relieving the need for highly defined substrates. Thus it was possible to compare DNA bending for specific and non-specific interactions. Non-specific complexes show no significant bending but increased rigidity compared to naked DNA, whereas specific complexes show DNA bending of on average 36°and higher flexibility. A model obtained by docking shows that photolyase can accommodate a 36°bent DNA in the vicinity of the active site