Effect of Work Enviroment and Work Motivation Employees Satisfaction Pdam Tirta Tuah Benua East Kutai

The main objective of this study was to determine the influence of working environment and work motivation on employees job satisfaction PDAM Tirta Tuah BenuaEast Kutai. The noun collected through questionnaires and conducted on 110 employees of PDAM Tirta Tuah Benua East Kutai periode of 2013. Analysis of the datain this study using SPSS version 16.0, the sampling technique used was simple random sampling and testing technique of noun used in this research include testing the validity of the factor analysis, the cronbachalpha reliability test with multiple linier regression analysis, to test and prove the research hypothesis.Analysis result showed that the count results helping by SPSS 16,0 Concluded that Fhit = 10,2 with 0,000 significant. Work miliew (X1) positive effect significant with work satisfied of PDAM Tirta Tuah Benua East Kutai worker. Work motivation (X) positive effect significant with work satisfied of PDAM Tirta Tuah Benua East Kutai worker. Work miliew (X1) and Work Motivation (X2) effect significant with work satisfied of PDAM Tirta Tuah Benua East Kutai.Hypothesis shows that H1 = Work Environment significant posititive effect on employee job satisfaction of PDAM Tirta Tuah Benua East Kutai.H2 = Work Motivation significant positive effect on employees job satisfaction of PDAM Tirta Tuah Benua East Kutai.H3 = Working Environment significant positive effect on employee job satisfaction of PDAM Tirta Tuah Benua East Kutai.Test sig t greater at 5% then there heterokedastisitas.Value F arithmatic performance of employees is greater than the F table ( 4,42) so that Ho is rejected and Ha be accepted.If significant level below 0,05 then the hypothesis be accepted.If level significanly greater than 0,05 then the hypothesis is rejected.Based on the anlysis,the authors provide suggestions for further research are expected to be examined by other variables beyond the variables that have been investigated in order to obtain a more varied results that can affect the job saticfaction of employees

Analysis of bovine herpesvirus-1 antibodies by enzyme immunoassay in Indonesian cattle

Enzyme-linked immunosorbent assay (ELISA) (HerdCheck: Anti-IBR) of 231 sera samples, all originated from Indonesian Holstein Friesian crossbred cattle with predetermined history of freedom from BHV-1 vaccination revealed that the ELISA was able to detect specific BHV-1 antibodies, estimated by testing 121, 65 and 45 sera showed the antibody positive sera of 11.6, 24.6 and 22.2%, respectively. Because no vaccination is allowed, it appears that the ELISA posive cattle may have been infected with IBR field strains. The ELISA for the routine detection of antibodies against BHV-1 in Indonesian cattle is recommended as screening for natural exposure to IBR infection in order to control and eradicate further risk of disease transmission. Keywords: ELISA (HerdCheck: Anti-IBR)-IBR-Holstein Friesia

RADIO RECEPTOR ASSAY FOR DIAGNOSTIC SERUM AND MILK 1,25-DIHYDROXYCLIOLECALCIFEROL

ABSTRACT: The objectives of this study were to modify and develop technique for determination of 1,25-(OHhD3 concentrations in sera and milk samples. The developed technique, used non-HPLC for extraction and purification of the samples and involved radio receptor assay which used calf thymus as 1,25- (OH)2D3 receptor for quantitation of 1,25-(011)203. Results of this study were compare to that of the technique developed by Horst a aL 0981) which used HPLC for samples extraction and purification. The correlation between the two techniques was 1.3,95. Using the modified technique the 1,25-(OHhD3 concentrations in cow and sow sera were 57.42 ± 53 pg/ml (n=20) and 75.22 ± 6.05 pg/m1 (n=20) respectively, whereas the 1,25-(OH)2D3 concentrations in cow and sow milk were 16.62 ± 2.2 pg/ml (n=20) and 21.52 ± 3.125 pgind (n=20). Based on the results could be concluded that the technique that had been developed in this study was found satisfactory for determination the 1,25-(OHhD3 concentration either in serum or milk samples

PENGARUH VARIASI IMBANGAN Ca:P DALAM PAKAN TERHADAP KADAR ALKALIN FOSFATASE, Ca DAN P DALAM DARAH DAN PERUBAHAN TULANG PADA TIKUS PUTIH (Rattus narvegicus albinus)

ABSTRAK: Dua puluh ekor tikus putih betina (Rattus norvegicus albinus) , umur 40 hari, digunakan dalam penelitian untuk mempelajari pengaruh variasi imbangan Ca : P dalam-Pakan terhadap kadar alkalin fosfatase, Ca dan p dalam darah serta perubahan tulang . Tikus tersebut kemudian secara acak dibagi dalam 4 kelompok (AB, C dan D), masing-masing 5 ekor. Pakan yang diberikan pada kelompok A ( kelompok kontrol) mengandung kadar kalsium dan fosfor dengan perbandingan 1,5 : 1, sedangkan pakan yang diperlukan pada tikus kelompok B, C dan D masing-masing mengandung⢠kadar kalsium dan fosfor dengan perbandingan 1,5 : 31,5 : 6 dan 1.5 : 9 , selama 2 bulan. Pada akhir perlakuan darah diambil melalui jantung untuk analisis kalsium, fosfat dan alkalin fosfatase. Tutang Lemur digunakan untuk pemeriksaan histopatologik. Pakan yang mengandung fosfor dan kalsium dengan perbandingan 1,5 : 6 menyebabkan peningkatan aktifitas remodelling tulang di bagian metafisis yang berbatasan dengan fisis. Pakan yang mengandung fosfor dan kalsium dengan perbandingan 1,5 : 9 menyebabkan osteodistrofi fibtosa, turunnya kadar kalsiurn darah, naiknya kadar fosfat dan alkalin fosfatase darah

Asuhan Keperawatan pada Tn.s dengan Gangguan Sensori Persepsi : Halusinasi Pendengaran di Ruang Merak RS Jiwa Dr.soeharto Heerdjan Jakarta

Keperawatan jiwa merupakan suatu bidang spesialisasi praktek keperawatan yang menerapkan teori prilaku manusia sebagai ilmunya dan penggunaan diri secara teraupetik sebagai kiatnya, salah satu dari gangguan yang terjadi pada gangguan jiwa orientasi realita yaitu ketidakmampuan klien berespon pada realitas, klien tidak dapat membedakan lamunan dan Kenyataan yang termasuk gangguan orientasi realita yaitu : waham, ilusi dan halusinasi. Tujuan umum penelitian ini adalah malakukan perawatan terhadap klien dengan gangguan sensori persepsi : halusinasi pendengaran. Penelitian ini menggunakan metode deskriptif. Tekhnik yang digunakan dalam mengumpulkan data,menganalisa data serta menarik kesimpulan yang kemudian disajikan dalam bentuk narasi. Cara menyusun laporan penelitian ini adalah Study Kepustakaan dan Study Kasus. Halusinasi adalah Perubahan sensori persepsi individu dalam menginterpretasikan sesuatu yang nyata tanparangsangan atau stimulus dari luar atau eksternal yang dialami oleh kelima panca indra. Dalam pengkajian ditemukan data yang sesuai dengan masalah Gangguan Sensori Persepsi : Halusinasi pendengaran, dan mendapatkan data faktor predisposisinya yaitu klien mengatakan dirinya mendengar suara–suara, suara–suara itu datang pada saat klien sedang menyendiri dan pada saat akan tidur malam, klien mengatakan suara–suara itu seperti suara perempuan yang memanggil–manggil dirinya. Hasil penelitian ini adalah Klien merasa kesal dan tidak suka mendengar suara–suara itu, saat melakukan pengkajian penulis tidak mendapat banyak hambatan, karena sikap klien yang kooperatif dan menerima saat berinteraksi. Diagnosa keperawatan pada Tn. S dari prioritas masalah utama adalah Gangguan Sensori Persepsi : Halusinasi pendengaran, adapun diagnosa yang lain berdasarkan data–data yang didapat yaitu, Isolasi sosial. Pada Implementasi keperawatan, penulis sudah melakukan sesuai dengan rencana yang telah dibuat, dan juga sudah melakukan implementasi untuk diagnosa utama yaitu Gangguan Sensori Persepsi : Halusinasi pendengaran. Evaluasi keberhasilan tindakan pada diagnosa utama sesuai dengan strategi pelaksanaan sudahtercapai dimana klien sudah menjalin hubungan saling percaya dengan perawat, klien mampu mengenal halusinasinya, klien mampu menghardik halusinasi, klien mampu melakukan cara baru mengendalikan halusinasi dengan cara bercakap–cakap dengan orang lain, klien juga mampu melakukan cara mengendalikan halusinasi dengan cara beraktivitas

Generating a restriction map of the amplified DNA of non-cytopathic bovine viral diarrhea virus of Indonesia isolate

The results of our previous investigation on the prevalence of antibodies to bovine viral diarrhea virus (BVDV) in cattle on rural farms in parts of Indonesia indicated that the prevalence of Indonesian cattle exposed to BVDV is considered to be significant. However, without a very good diagnostic assay to detect persistently infected animals it is not possible to determine the pathogenesis and the etiologic role of this BVDV in affecting cattle production as well as to apply the program allowing efficient identification and elimination of BVDV infected cattle from the herd in Indonesia. The approach of reverse transcription (RT) followed by the polymerase chain reaction (PCR) was used to amplify a 482 base pair fragment of the non-cytopathic BVDV (NCP-BVDV) genome. Two sets of primers framed two different regions within the gene coding for protein p80. NCP-BVDV RNA extracted from infected bovine turbinate cells could be detected using RT-PCR. Size specific amplification products was confirmed by restriction endonuclease analysis of the RT-PCR products to be BVDV. The sensitivity of this method might be of relevance for diagnostic purposes of infection with BVDV

Integrasi Kearifan Lokal Baduy Pada Pengembangan Bahan Ajar Modul IPA Dalam Menanamkan Nilai-Nilai Konservasi Lingkungan

The existence of globalization and modernization is the cause of various changes in a life, one of which is local wisdom. Local wisdom is now fading along with the rapid development of the times. The process of learning at the basic education level is to use the 2013 curriculum with integrative thematic learning, namely a learning model which consists of various themes in an integrated manner so that a meaningful experience can be obtained by students. The 2013 curriculum is based on cultural appreciation or local wisdom as stated in Permendikbud Nomor 79 Tahun 2014 concerning Local Content for the 2013 Curriculum. Teaching materials for thematic books are used nationally so that there is not enough local cultural wisdom for students. In the 2013 curriculum, science is one of the compulsory subjects. The principle of science learning is scientific inquiry with the surrounding environment, so it is very suitable if there is the involvement of Baduy local wisdom because in it Baduy is one of the Indonesian ethnic groups who are very close to nature. Therefore, researchers designed teaching materials in which there is local Baduy wisdom in an effort to instill environmental conservation values. In conducting this research, the researcher uses the R&D method, namely the ADDIE model with five stages. The teaching materials have been validated by material experts and media experts and given an assessment by the teacher which is stated in the appropriate category and without revision. The teaching materials developed were effective because all respondents gave positive responses and there was an increase in student learning outcomes before and after using the product

Pengaruh Lingkungan Kerja dan Motivasi Kerja terhadap Kepuasan Kerja Karyawan Pdam Tirta Tuah Benua Kutai Timur

The main objective of this study was to determine the influence of working environment and work motivation on employees job satisfaction PDAM Tirta Tuah BenuaEast Kutai. The noun collected through questionnaires and conducted on 110 employees of PDAM Tirta Tuah Benua East Kutai periode of 2013. Analysis of the datain this study using SPSS version 16.0, the sampling technique used was simple random sampling and testing technique of noun used in this research include testing the validity of the factor analysis, the cronbachalpha reliability test with multiple linier regression analysis, to test and prove the research hypothesis.Analysis result showed that the count results helping by SPSS 16,0 Concluded that Fhit = 10,2 with 0,000 significant. Work miliew (X1) positive effect significant with work satisfied of PDAM Tirta Tuah Benua East Kutai worker. Work motivation (X) positive effect significant with work satisfied of PDAM Tirta Tuah Benua East Kutai worker. Work miliew (X1) and Work Motivation (X2) effect significant with work satisfied of PDAM Tirta Tuah Benua East Kutai.Hypothesis shows that H1 = Work Environment significant posititive effect on employee job satisfaction of PDAM Tirta Tuah Benua East Kutai.H2 = Work Motivation significant positive effect on employees job satisfaction of PDAM Tirta Tuah Benua East Kutai.H3 = Working Environment significant positive effect on employee job satisfaction of PDAM Tirta Tuah Benua East Kutai.Test sig t greater at 5% then there heterokedastisitas.Value F arithmatic performance of employees is greater than the F table ( 4,42) so that Ho is rejected and Ha be accepted.If significant level below 0,05 then the hypothesis be accepted.If level significanly greater than 0,05 then the hypothesis is rejected.Based on the anlysis,the authors provide suggestions for further research are expected to be examined by other variables beyond the variables that have been investigated in order to obtain a more varied results that can affect the job saticfaction of employees