Genome-Wide Analysis of the PIN Auxin Efflux Carrier Gene Family in Coffee

Coffee is one of the most popular beverages around the world, which is mainly produced from the allopolyploid Coffea arabica. The genomes of C. arabica and its two ancestors C. canephora and C. eugenioides have been released due to the development of next generation sequencing. However, few studies on C. arabica are related to the PIN-FORMED (PIN) auxin efflux transporter despite its importance in auxin-mediated plant growth and development. In the present study, we conducted a genome-wide analysis of the PIN gene family in the three coffee species. Totals of 17, 9 and 10 of the PIN members were characterized in C. Arabica, C. canephora and C. eugenioides, respectively. Phylogenetic analysis revealed gene loss of PIN1 and PIN2 homologs in C. arabica, as well as gene duplication of PIN5 homologs during the fractionation process after tetraploidy. Furthermore, we conducted expression analysis of PIN genes in C. arabica by in silico and qRT-PCR. The results revealed the existence of gene expression dominance in allopolyploid coffee and illustrated several PIN candidates in regulating auxin transport and homeostasis under leaf rust fungus inoculation and the tissue-specific expression pattern of C. arabica. Together, this study provides the basis and guideline for future functional characterization of the PIN gene family

An Investigation of a Confirmed Imported Case of COVID-19 Infected Abroad - Qingpu District, Shanghai Municipality, China, November 15, 2020.

SummaryWhat is already known about this topic? A passenger who was from the United States was taken to the hotel for the required isolation on November 13, 2020. During the quarantine she was diagnosed as the COVID-19 patient on November 15, 2020. Controlling the importation of COVID-19 remains a major challenge.What is added by this report? In this study, an epidemiological investigation was conducted for a confirmed case of COVID-19, including the treatment records in the hospital and 14-day travel trajectory before the onset of disease.What are the implications for public health practice? This study described an epidemiological investigation and management process on an imported case of COVID-19 and analyzed the test results, aiming to provide useful warnings to strengthen the capacity of public health system in response to the importation

Development of the injector II RFQ for China ADS project

As one of the main components of the injector II of China ADS LINAC project, an RFQ working at 162.5MHz is used to accelerate proton beams of 15mA from 30 keV to 2.1 MeV. The four vane RFQ has been designed in collaboration with Lawrence Berkeley National Laboratory and built at the workshop of the Institute of Modern Physics, Chinese Academy of Sciences (IMP, CAS). Low power test of the cavity have been completed, and it shows the field flatness is within ±1% and the unloaded Q is 12600. RF conditioning has been completed, results of preliminary beam test show the output beam energy is 2.16 MeV with energy spread of 3.5% and the transmission efficiency is 97.9%. Continuous wave (CW) beam of 2.3 mA has been accelerated for more than one hour