DeftectNet: Joint loss structured deep adversarial network for thermography defect detecting system

In this paper, a novel joint loss Generative Adversarial Networks (GAN) framework is proposed for thermography nondestructive testing named Defect-Detection Network (DeftectNet). A new joint loss function that incorporates both the modified GAN loss and penalty loss is proposed. The strategy enables the training process to be more stable and to significantly improve the detection rate. The obtained result shows that the proposed joint loss can better capture the salient features in order to improve the detection accuracy. In order to verify the effectiveness and robustness of the proposed method, experimental studies have been carried out for inner debond defects on both regular and irregular shaped carbon fiber reinforced polymer/plastic (CFRP) specimens. A comparison experiment has been undertaken to study the proposed method with other current state-of-the-art deep semantic segmentation algorithms. The promising results have been obtained where the performance of the proposed method can achieve end-to-end detection of defects

Structured iterative alternating sparse matrix decomposition for thermal imaging diagnostic system

In this paper, we propose a structured iterative alternating sparse matrix decomposition to efficiently decompose the input multidimensional data from active thermography into the sum of a low-rank matrix, a sparse matrix, and a noise matrix. In particular, the sparse matrix is further factorized into a pattern constructed dictionary matrix and a coefficient matrix. The estimation of the dictionary matrix and coefficient matrix is based on integrating the vertex component analysis with the framework of the alternating direction method of multipliers. In addition, the joint structure sparsity and nonnegative constraint are emphasized as part of the learning strategy. In order to verify the effectiveness and robustness of the proposed method, experimental studies have been carried out by applying the proposed method to thermal imaging diagnostic system for carbon fiber reinforced plastics (CFRP) defects detections. The validation study has been conducted by comparing the proposed method with the current state-of-the-art algorithms. The results indicate that the proposed method significantly improves the contrast ratio between the defective regions and the non-defective regions

Coupled Deep-Mantle Carbon-Water Cycle: Evidence From Lower-Mantle Diamonds

Diamonds form in a variety of environments between subducted crust, lithospheric and deep mantle. Recently, deep source diamonds with inclusions of the high-pressure H O-phase ice-VII were discovered. By correlating the pressures of ice-VII inclusions with those of other high-pressure inclusions, we assess quantitatively the pressures and temperatures of their entrapment. We show that the ice-VII-bearing diamonds formed at depths down to 800 ± 60 km but at temperatures 200–500 K below average mantle temperature that match the pressure-temperature conditions of decomposing dense hydrous mantle silicates. Our work presents strong evidence for coupled recycling of water and carbon in the deep mantle based on natural samples.

Nickel Isotopic Evidence for Late-Stage Accretion of Mercury-Like Differentiated Planetary Embryos

© 2021, The Author(s). Earth’s habitability is closely tied to its late-stage accretion, during which impactors delivered the majority of life-essential volatiles. However, the nature of these final building blocks remains poorly constrained. Nickel (Ni) can be a useful tracer in characterizing this accretion as most Ni in the bulk silicate Earth (BSE) comes from the late-stage impactors. Here, we apply Ni stable isotope analysis to a large number of meteorites and terrestrial rocks, and find that the BSE has a lighter Ni isotopic composition compared to chondrites. Using first-principles calculations based on density functional theory, we show that core-mantle differentiation cannot produce the observed light Ni isotopic composition of the BSE. Rather, the sub-chondritic Ni isotopic signature was established during Earth’s late-stage accretion, probably through the Moon-forming giant impact. We propose that a highly reduced sulfide-rich, Mercury-like body, whose mantle is characterized by light Ni isotopic composition, collided with and merged into the proto-Earth during the Moon-forming giant impact, producing the sub-chondritic Ni isotopic signature of the BSE, while delivering sulfur and probably other volatiles to the Earth

Buyang Huanwu Decoction Attenuates Infiltration of Natural Killer Cells and Protects Against Ischemic Brain Injury

Background/Aims: Natural killer (NK) cells are among the first immune cells that respond to an ischemic insult in human brains. The infiltrated NK cells damage blood-brain barrier (BBB) and exacerbate brain infarction. Buyang Huanwu Decoction (BHD), a classic Chinese traditional herbal prescription, has long been used for the treatment of ischemic stroke. The present study investigated whether BHD can prevent brain infiltration of NK cells, attenuate BBB disruption and improve ischemic outcomes. Methods: Transient focal cerebral ischemia was induced in rats by a 60-minute middle cerebral artery occlusion, and BHD was orally administrated at the onset of reperfusion, 12 hours later, then twice daily. Assessed parameters on Day 3 after ischemia were: neurological and motor functional deficits through neurological deficit score and rotarod test, respectively; brain infarction through TTC staining; BBB integrity through Evans blue extravasation; matrix metalloproteinase-2/9 activities through gelatin zymography; tight junction protein, nuclear factor-kB (NF-kB) p65 and phospho-p65 levels through Western blotting; NK cell brain infiltration and CXCR3 levels on NK cells through flow cytometry; interferon-γ production through ELISA; CXCL10 mRNA levels through real-time PCR; CXCL10 expression and p65 nuclear translocation through immunofluorescence staining. Results: BHD markedly reduced brain infarction, improved rotarod performance, and attenuated BBB breakdown. Concurrently, BHD attenuated the upregulation of matrix metalloproteinase-2/9 activities and the degradation of tight junction proteins in the ischemic brain. Infiltration of NK cells was observed in the ischemic hemisphere, and this infiltration was blunted by treatment with BHD. BHD suppressed brain ischemia-induced interferon-γ and chemokine CXCL10 production. Furthermore, BHD significantly reduced the expression of CXCR3 on brain-infiltrated NK cells. Strikingly, BHD did not affect NK cell levels or its CXCR3 expression in the spleen or peripheral blood after brain ischemia. The nuclear translocation of NF-kB p65 and phospho-p65 in the ischemic brain was inhibited by BHD. Conclusion: Our findings suggest that BHD prevents brain infiltration of NK cells, preserves BBB integrity and eventually improves ischemic outcomes. The inhibitory effects of BHD on NK cell brain invasion may involve its ability of suppressing NF-kB-associated CXCL10-CXCR3-mediated chemotaxis. Notably, BHD only suppresses NK cells and their CXCR3 expression in the ischemic brain, but not those in periphery

Non-invasive detection of lymphoma with circulating tumor DNA features and protein tumor markers

BackgroundAccording to GLOBOCAN 2020, lymphoma ranked as the 9th most common cancer and the 12th leading cause of cancer-related deaths worldwide. Traditional diagnostic methods rely on the invasive excisional lymph node biopsy, which is an invasive approach with some limitations. Most lymphoma patients are diagnosed at an advanced stage since they are asymptomatic at the beginning, which has significantly impacted treatment efficacy and prognosis of the disease.MethodThis study assessed the performance and utility of a newly developed blood-based assay (SeekInCare) for lymphoma early detection. SeekInCare utilized protein tumor markers and a comprehensive set of cancer-associated genomic features, including copy number aberration (CNA), fragment size (FS), end motif, and lymphoma-related virus, which were profiled by shallow WGS of cfDNA.ResultsProtein marker CA125 could be used for lymphoma detection independent of gender, and the sensitivity was 27.8% at specificity of 98.0%. After integrating these multi-dimensional features, 77.8% sensitivity was achieved at specificity of 98.0%, while its NPV and PPV were both more than 92% for lymphoma detection. The sensitivity of early-stage (I-II) lymphoma was up to 51.3% (47.4% and 55.0% for stage I and II respectively). After 2 cycles of treatment, the molecular response of SeekInCare was correlated with the clinical outcome.ConclusionIn summary, a blood-based assay can be an alternative to detect lymphoma with adequate performance. This approach becomes particularly valuable in cases where obtaining tissue biopsy is difficult to obtain or inconclusive

A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts

Terminally differentiated cells can be generated by lineage reprogramming, which is, however, hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming.</p

Long-term functional maintenance of primary human hepatocytes in vitro

The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening.</p

Investigations on the micellization of amphiphilic dendritic copolymers: from unimers to micelles

Since the micellization kinetics is influenced by polymer structure, the spherical three-dimensional topology of amphiphilic dendritic copolymers (ADPs) which hinders the phase separation during micellization is assumed to make the micellization kinetics different. In the literatures, most of the attention has been paid to the morphology transition or the morphology at equilibrium and the micellization kinetics of ADPs is rarely reported. In this study, the micellization processes of amphiphilic dendritic copolymers from unimers to the final equilibrium micelles were monitored by laser light scattering. Based on the closed association mechanism, the thermodynamics of micellization was analysed. The negative thermodynamic quantities indicate that the micellization of ADPs is driven by enthalpy. Based on the change of scattering intensity and hydrodynamic radius (Rh) with time, the detailed micellization kinetics was analysed, which contains two steps. By controlling the temperature and type of solvent, a system in which the concentration has little influence on Rh is obtained. The relaxation times of the two steps decrease with concentration, indicating that at higher concentration the rate of micellization is quicker. With the increasing mass fraction of the hydrophobic part, the relaxation times decrease and the driving force of micellization increases