Isolation and Characterization of European Foulbrood Antagonistic Bacteria from the Gastrointestine of the Japanese Honeybee Apis cerana japonica

筑波大学 (University of Tsukuba)201

Proper orthogonal decomposition and recurrence map for the identification of spatial–temporal patterns in a low-Re wake downstream of two cylinders

Flow decomposition methods provide systematic ways to extract the flow modes, which can be regarded as the spatial distribution of a coherent structure. They have been successfully used in the study of wake, boundary layer, and mixing. However, real flow structures also possess complex temporal patterns that can hardly be captured using the spatial modes obtained in the decomposition. In order to analyze the temporal variation of coherent structures in a complex flow field, this paper studies the recurrence in phase space to identify the pattern and classify the evolution of the flow modes. The recurrence pattern depends on the time delay and initial condition. In some cases, the flow system will revisit a previous state regardless of the initial state, and in other cases, the system’s recurrence will depend on the initial state. These patterns are determined by the arrangement and interactions of coherent structures in the flow. The temporal order of the repetition pattern reflects the possible ways of flow evolution

Proper Orthogonal Decomposition and Recurrence Map for the Identification of Spatial–Temporal Patterns in a Low-Re Wake Downstream of Two Cylinders

Flow decomposition methods provide systematic ways to extract the flow modes, which can be regarded as the spatial distribution of a coherent structure. They have been successfully used in the study of wake, boundary layer, and mixing. However, real flow structures also possess complex temporal patterns that can hardly be captured using the spatial modes obtained in the decomposition. In order to analyze the temporal variation of coherent structures in a complex flow field, this paper studies the recurrence in phase space to identify the pattern and classify the evolution of the flow modes. The recurrence pattern depends on the time delay and initial condition. In some cases, the flow system will revisit a previous state regardless of the initial state, and in other cases, the system’s recurrence will depend on the initial state. These patterns are determined by the arrangement and interactions of coherent structures in the flow. The temporal order of the repetition pattern reflects the possible ways of flow evolution

Isolation and Characterization of European Foulbrood Antagonistic Bacteria from the Gastrointestine of the Japanese Honeybee Apis cerana japonica

この博士論文は一部が非公開になっています筑波大学 (University of Tsukuba)201

The current status of Hospital Information Systems in Yanbian, China

Purpose: The purpose of this study was to analyze the current status of Hospital Information system in Yanbian, China. Methods: Structured self-administered questionnaires were sent to hospitals in Yanbian to survey the issues and six hospitals answered. Data were analyzed by using Windows SPSS 12.0. Results: All facilities were second-grade national hospitals with 100~500 beds, kept about 40 computers and all used internet. Four of them had information exclusive department, and the other two had the implementation plans. All of the hospitals had Patient Billing System and some features of Medication Administration System. Four hospitals had administration systems, and the other two had no plans to develop or adopt administration system in the next three years. Two hospitals used the packages composed of Electronic Data Interchange System, Amount Receivable Management System and Laboratory Information System. One hospital used the Picture Archiving and Communication System, Telemedicine System, Radioactive Examination. Conclusions: Compared with Korea, the results showed relevant explanation on the delays of implementing HIS and its current status in Yanbian. In order to develop Hospital Information System, various strategies must be developed and active international support and research was required to provide the appropriate experiences.OAIID:oai:osos.snu.ac.kr:snu2009-01/102/0000028528/1SEQ:1PERF_CD:SNU2009-01EVAL_ITEM_CD:102USER_ID:0000028528ADJUST_YN:NEMP_ID:A076124DEPT_CD:811CITE_RATE:0FILENAME:23 The Current Status of Hospital Information Systems in Yanbian, China.pdfDEPT_NM:간호학과EMAIL:[email protected]_YN:NCONFIRM:

Agenda 21 and the ecological footprint as instruments to achieve a sustainable university

[Resumen] Para frenar el rápido deterioro ambiental, la universidad ha de formar a las nuevas generaciones en un modelo de sostenibilidad integral (solidaridad sincrónica y diacrónica) y, además, contribuir al cambio en los estilos de vida, en los conocimientos y conductas de la sociedad actual. Esto precisa un nuevo marco teórico-metodológico bajo el paradigma de la sostenibilidad que fundamente y concrete las propuestas educativas. Tomando como referencia los principios del desarrollo sostenible y utilizando como «herramientas» la Agenda 21en la Universidad y la Huella Ecológica, hemos desarrollado y puesto en práctica una estrategia metodológica (ecometodología) con el fin de capacitar a nuestro alumnado (profesorado en formación) para actuar con criterios de sustentabilidad, tanto colectiva, como individualmente[Abstract] In order to stop the quick environmental deterioration of the planet, University has to prepare the new generations following a model of integral sustainability (synchronic and diachronic solidarity) and it also has to contribute to a change in lifestyles, in knowledge and behaviours in our society. There is a need for a new theoretical-methodological frame under the paradigm of sustainability to base educational proposals on it and to concretize them. Taking into consideration the principles of Sustainable Development and using the tools of «Agenda 21 in the University» and Ecological Footprint, we have developed and applied a methodological strategy (ecomethodology) to enable ours students (future teachers) to act, collectively and individually, according to the criteria of sustainabilit

Causal effects from inflammatory bowel disease on liver function and disease: a two-sample Mendelian randomization study

BackgroundAccumulating evidence has shown that patients with inflammatory bowel disease (IBD) have liver function abnormalities and are susceptible to liver diseases. However, the existence of a causal relationship between IBD and liver function or disease remains unclear.MethodsA two-sample Mendelian randomization (MR) analysis was performed using genetic associations from publicly available genome-wide association studies (GWAS). These associations encompass ulcerative colitis (UC), Crohn’s disease (CD), liver function traits, and liver disease phenotypes. The liver function traits comprised hepatic biochemistries, percent liver fat, and liver iron content from the UK Biobank. Furthermore, the liver disease phenotypes included cholelithiasis, non-alcoholic fatty liver disease (NAFLD), primary sclerosing cholangitis (PSC), and primary biliary cholangitis (PBC) in cohorts of European ancestry. The primary estimation used the inverse-variance weighted method, with GWAS of C-reactive protein (CRP) in the UK Biobank serving as a positive control outcome.ResultsGenetically predicted UC is causally associated with decreased levels of albumin (ALB) and liver iron content, while genetically predicted CD is causally associated with increased levels of alkaline phosphatase (ALP). Moreover, genetically predicted UC or CD increases the risk of PSC, and CD increases the risk of PBC. Neither UC nor CD causally increases the risk of cholelithiasis and NAFLD.ConclusionUC affects the levels of ALB and liver iron content, while CD affects the levels of ALP. Both UC and CD increase the risk of PSC, and CD increases the risk of PBC

Mucosal and Systemic Immune Responses to Influenza H7N9 Antigen HA1–2 Co-Delivered Intranasally with Flagellin or Polyethyleneimine in Mice and Chickens

Consecutive cases of human infection with H7N9 influenza viruses since 2013 in China have prompted efforts to develop an effective treatment. Subunit vaccines introduced by intranasal administration can block an infection at its primary site; flagellin (fliC) and polyethyleneimine (PEI) have been shown to be potent adjuvants. We previously generated the hemagglutinin (HA)1–2-fliC fusion protein consisting of the globular head domain (HA1–2; amino acids 62–284) of HA fused with Salmonella typhimurium fliC. In the present study, we investigated its effectiveness of both flagellin and PEI as mucosal adjuvants for the H7N9 influenza subunit vaccine. Mice immunized intranasally with HA1–2-fliC and HA1–2-PEI showed higher HA1–2-specific immunoglobulin (Ig)G and IgA titers in serum, nasal wash, and bronchial alveolar lavage fluid. Moreover, splenocyte activation and proliferation and the number of HA1–2-specific interferon (IFN)-γ- and interleukin (IL)-4-producing splenocytes were markedly increased in the fliC and PEI groups; in the latter, there were more cells secreting IL-4 than IFN-γ, suggesting that fliC induced T helper type (Th)1 and Th2 immune responses, and PEI induced Th2-biased responses, consistent with the serum antibody isotype pattern (IgG1/IgG2a ratio). Furthermore, virus challenge was performed in a chicken model. The results showed that chickens receiving fliC and PEI adjuvant vaccine exhibited robust immune responses leading to a significant reduction in viral loads of throat and cloaca compared to chickens receiving only HA1–2. These findings provide a basis for the development of H7N9 influenza HA1–2 mucosal subunit vaccines

Liver-Targeted Combination Therapy Basing on Glycyrrhizic Acid-Modified DSPE-PEG-PEI Nanoparticles for Co-delivery of Doxorubicin and Bcl-2 siRNA

Combination therapy based on nano-sized drug delivery system has been developed as a promising strategy by combining two or more anti-tumor mechanisms. Here, we prepared liver-targeted nanoparticles (GH-DPP) composed of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-polyethylene glycol-polyetherimide (DSPE-PEG-PEI) with Glycyrrhetinic acid-modified hyaluronic acid (GA-HA) for co-delivery of doxorubicin (DOX) and Bcl-2 siRNA. Particles size, zeta potential and morphology were determined for the drug-loaded GH-DPP nanoparticles (siRNA/DOX/GH-DPP). Cellular uptake and in vitro cytotoxicity were analyzed against HepG2 cells. In vivo bio-distribution and anti-tumor therapeutic effects of siRNA/DOX/GH-DPP were evaluated in H22-bearing mice. The results showed that siRNA/DOX/GH-DPP nanoparticles were nearly spherical and showed dose-dependent cytotoxicity against HepG2 cells. Compared to Glycyrrhetinic acid-free co-delivery system (siRNA/DOX/DPP) and GH-DPP nanoparticles for delivery of DOX or Bcl-2 siRNA alone, siRNA/DOX/GH-DPP nanoparticles could induce more cellular apoptosis, and showed higher anti-tumor effect. Herein GH-DPP nanoparticles could simultaneously deliver both chemotherapy drugs and siRNA into the tumor region, exhibiting great potential in anti-tumor therapy