Apolipoprotein e mediates attachment of clinical hepatitis C virus to hepatocytes by binding to cell surface heparan sulfate proteoglycan receptors

Our previous studies demonstrated that the cell culture-grown hepatitis C virus of genotype 2a (HCVcc) uses apolipoprotein E (apoE) to mediate its attachment to the surface of human hepatoma Huh-7.5 cells. ApoE mediates HCV attachment by binding to the cell surface heparan sulfate (HS) which is covalently attached to the core proteins of proteoglycans (HSPGs). In the present study, we further determined the physiological importance of apoE and HSPGs in the HCV attachment using a clinical HCV of genotype 1b (HCV1b) obtained from hepatitis C patients and human embryonic stem cell-differentiated hepatocyte-like cells (DHHs). DHHs were found to resemble primary human hepatocytes. Similar to HCVcc, HCV1b was found to attach to the surface of DHHs by the apoE-mediated binding to the cell surface HSPGs. The apoE-specific monoclonal antibody, purified HSPGs, and heparin were all able to efficiently block HCV1b attachment to DHHs. Similarly, the removal of heparan sulfate from cell surface by treatment with heparinase suppressed HCV1b attachment to DHHs. More significantly, HCV1b attachment was potently inhibited by a synthetic peptide derived from the apoE receptor-binding region as well as by an HSPG-binding peptide. Likewise, the HSPG-binding peptide prevented apoE from binding to heparin in a dose-dependent manner, as determined by an in vitro heparin pull-down assay. Collectively, these findings demonstrate that HSPGs serve as major HCV attachment receptors on the surface of human hepatocytes to which the apoE protein ligand on the HCV envelope binds

Wake-up timer and binary exponential backoff for ZigBee-based wireless sensor network for flexible movement control system of a self-lifting scaffold

Synchronous movement of attached self-lifting scaffolds is traditionally monitored with wired sensors in high-rise building construction, which limits their flexibility of movements. A ZigBee-based wireless sensor system has been suggested in this article to prove the effectiveness of wireless sensor networks in actual implementation. Two optoelectronic sensors are integrated into a ZigBee node for measuring the displacement of attached self-lifting scaffolds. The proposed wireless sensor network combines an end device and a coordinator to allow easy replacement of sensors as compared to a wired network. A wake-up timer algorithm is proposed to reduce the transmitting power during continuous wireless data communication in the wireless sensor network. Furthermore, a variant binary exponential backoff transmission algorithm for data loss avoidance is proposed. The variant binary exponential backoff algorithm reduces packet collisions during simultaneous access by increasing the randomizing moments at nodes attempting to access the wireless channels. The performance of three of the proposed modules - a cable sensor, a 315-MHz sensor, and a ZigBee sensor - is evaluated in terms of packet delivery ratio and the end-to-end delay of a ZigBee-based wireless sensor network. The experimental results show that the proposed variant binary exponential backoff transmission algorithm achieves a higher packet delivery ratio at the cost of higher delays. The average cost of the developed ZigBee-based wireless sensor network decreased by 24% compared with the cable sensor. The power consumption of ZigBee is approximately 53.75% of the 315-MHz sensor. The average current consumption is reduced by approximately 1.5 mA with the wake-up timer algorithm at the same sampling rate. © The Author(s) 2016

Genetic diversity and phylogenetic analyses of 11 cohorts of captive rhesus macaques from Chinese zoos

Rhesus macaques are raised in almost every Chinese zoo due to their likeability and ease in feeding; however, little is yet known about the genetic diversity of rhesus macaques in captivity. In this study, a 475-base pair nucleotide sequence of the mitochondrial DNA control region was obtained from the fecal DNA of 210 rhesus macaque individuals in captivity. A total of 69 haplotypes were defined, 51 of which (73.9%) were newly identified. Of all haplotypes, seven were shared between two zoos, and 62 haplotypes (89.8%) appeared only in a specific zoo, indicating a low rate of animal exchange between Chinese zoos. Moreover, there was a relatively high level of genetic diversity among the rhesus macaques (Hd = 0.0623 ± 0.0009, Pi = 0.979 ± 0.003, K = 28.974). Phylogenetic analysis demonstrated that all haplotypes were clearly clustered into two major haplogroups—Clade A (southeastern China) and Clade B (southwestern China)—and each major clade contained several small sub-haplogroups. The haplotypes of rhesus macaques from the same zoo were not clustered together for the most part, but scattered among several subclades on the phylogenetic tree. This indicates that the rhesus macaques in most Chinese zoos may originat from a diverse collection of geographical areas. Our results demonstrate that zoos play an important role in the conservation of the genetic diversity of rhesus macaques, as well as provide useful information on the genetic management of captive rhesus macaques

Gut Microbes Reveal Pseudomonas Medicates Ingestion Preference via Protein Utilization and Cellular Homeostasis Under Feed Domestication in Freshwater Drum, Aplodinotus grunniens

With strong demand for aquatic products, as well as a rapid decrease in global fishery resources and capture fisheries, domesticating animals to provide more high-quality proteins is meaningful for humans. Freshwater drum (Aplodinotus grunniens) is widely distributed in the wild habitats of North America. However, the research on A. grunniens and the feed domestication with diets composed of artificial compounds remains unclear. In this study, a 4-month feeding domestication experiment was conducted with A. grunniens larvae to evaluate the underlying mechanism and molecular targets responsible for alternations in the ingestion performance. The results indicated that a significant increase in the final body weight was exhibited by the feed domesticated group (DOM, 114.8 g) when compared to the group that did not ingest the feed (WT, 5.3 g) as the latest version we raised From the result, the final body weight exhibited significant increase between unfavorable with the feed (WT, 5.3 g) and feed domesticated group (DOM, 114.8 g). In addition, the enzyme activity of digestive enzymes like amylase, lipase, and trypsin was increased in DOM. Genes related to appetite and perception, such as NPY4R, PYY, and LEPR, were activated in DOM. 16s rRNA gene sequencing analysis revealed that Pseudomonas sp. increased from 58.74% to 89.77% in DOM, which accounts for the dominant upregulated microbial community at the genus level, followed by Plesiomonas. Analogously, Mycobacterium, Methylocystis, and Romboutsia also accounted for the down-regulated microbes in the diversity. Transcriptome and RT-PCR analysis revealed that feed domestication significantly improved protein digestion and absorption, inhibited apoptosis by AGE-RAGE signaling, and activated extracellular matrix remodeling by relaxin signaling. Integrated analysis of the microbiome and host transcriptome revealed that Pseudomonas-mediated ingestion capacity, protein utilization, and cellular homeostasis might be the underlying mechanism under feed domestication. These results indicate Pseudomonas and its key genes relating to food ingestion and digestion could serve as the molecular targets for feed domestication and sustainable development in A. grunniens

Individual Professional Practice in the Company

Import 23/08/2017Cílem této bakalářské práce je popsat absolvování odborné praxe ve firmě HS Interactive s.r.o. Praxe byla zaměřena na vývoj mobilní aplikace pro operační systém Android. Aplikace je mobilním klientem pro sociální síť MatchToMe. V úvodu popisuji důvody, které vedly k výběru odborné praxe. Dále se věnuji úkolům, které mi byly zadány s jejich implementací a postupem řešení problémů, které se objevily při vývoji. Závěr práce je věnován zhodnocení získaných zkušeností a dosažených výsledků.Purpose of this bachelor thesis is to describe a professional practice in company HS Interactive s.r.o. Practice was focused on the development of mobile application for the operating system Android. The application is a mobile client for social network MatchToMe. In the introduction I describe reasons that led to the selection of professional practice. Then I describe tasks that I have been awarded with their implementations and process of solution issues that have emerged during development. The conclusion of thesis is dedicated to the evaluation of the experience gained and the results achieved.440 - Katedra telekomunikační technikyvýborn

Productive Hepatitis C Virus Infection of Stem Cell-Derived Hepatocytes Reveals a Critical Transition to Viral Permissiveness during Differentiation

Primary human hepatocytes isolated from patient biopsies represent the most physiologically relevant cell culture model for hepatitis C virus (HCV) infection, but these primary cells are not readily accessible, display individual variability, and are largely refractory to genetic manipulation. Hepatocyte-like cells differentiated from pluripotent stem cells provide an attractive alternative as they not only overcome these shortcomings but can also provide an unlimited source of noncancer cells for both research and cell therapy. Despite its promise, the permissiveness to HCV infection of differentiated human hepatocyte-like cells (DHHs) has not been explored. Here we report a novel infection model based on DHHs derived from human embryonic (hESCs) and induced pluripotent stem cells (iPSCs). DHHs generated in chemically defined media under feeder-free conditions were subjected to infection by both HCV derived in cell culture (HCVcc) and patient-derived virus (HCVser). Pluripotent stem cells and definitive endoderm were not permissive for HCV infection whereas hepatic progenitor cells were persistently infected and secreted infectious particles into culture medium. Permissiveness to infection was correlated with induction of the liver-specific microRNA-122 and modulation of cellular factors that affect HCV replication. RNA interference directed toward essential cellular cofactors in stem cells resulted in HCV-resistant hepatocyte-like cells after differentiation. The ability to infect cultured cells directly with HCV patient serum, to study defined stages of viral permissiveness, and to produce genetically modified cells with desired phenotypes all have broad significance for host-pathogen interactions and cell therapy

Characterization of rectilinear ion traps and extension of an ion trajectory simulation program to electrode configurations with arbitrary geometries

The objective of the thesis is to fully apply a computational simulation tool, the program Ion Trajectory SIMulation program ITSIM, to elucidate the complex ion motion inside a mass spectrometer system, so facilitating instrumentation development and guiding designs for new experiments. This required the extension of the capabilities of the program and experiments on a variety of ion trap instruments. A mass analyzer based on a rectilinear geometry ion trap (RIT) has been built and its performance has been characterized. Design concepts for RITs are delineated with emphasis on the effects of electrode geometry on the calculated electric field and an appropriate distribution of higher-order electric field contributions allows selection of an optimized geometry. The demonstrated capabilities of the RIT include large ion trapping capacity, tandem mass spectrometry, a mass resolution in excess of 1000, and a mass/charge range of 650 Th. An optimization procedure has been developed for the cylindrical ion trap (CIT) based on field calculations and simulations of ion motion. The geometrical effects were systematically investigated and optimized. Appropriate compensation by introducing high-order field components, namely octapolar and dodecapolar fields, was used to refine the CIT geometry and so to improve mass resolution in mass scans using boundary and resonance ejection. Design considerations for the miniature ion trap are also discussed in detail in terms of pseudopotential well depth, trapping efficiency, pressure and space charge effects. An improved multi-particle ion trajectory simulation program, ITSIM 6.0, is described which is capable of ion trajectory simulations for electrode configurations with arbitrary geometries. The electrode structures are input from a 3D drawing program AutoCAD and the electric field is calculated using a 3D field solver and ITSIM 6.0. It converts the calculated electric field into a field array file readable by ITSIM 6.0 and ion trajectories are calculated by solving Newton\u27s equation using Runge-Kutta methods. Simulations of ion motion in a variety of 3D devices, e.g. ion transport systems and linear ion traps, demonstrate both the qualitative and quantitative characteristics of ITSIM 6.0

Geochemical characteristics and genetic types of natural gas in the Changxing-Feixianguan Formations from the Yuanba Gas Field in the Sichuan Basin, China

The Yuanba Gas Field is a large gas field in the marine strata with the largest burial depth in China up to date. The studies performed on the mentioned gas field have achieved significant progress made possible by determining the characteristics of reef flat reservoirs in the marine strata and the main controlling factors of hydrocarbon accumulation. However, there is no consensus on the origin of natural gases stored in the reservoir in the Changxing-Feixianguan Formations in the Yuanba and its adjacent area. The study on geochemical characteristics indicates that natural gases in the reservoir in the Changxing-Feixianguan Formations in the Yuanba Gas Field are mainly composed of alkane gases which are dominated by methane with dryness coefficients generally higher than 0.995. The CO2 and H2S display an average content of 8.55% and 6.47%, respectively. The δ13C1 and δ13C2 values are from −31.2‰ to −27.9‰, and from −29.9‰ to −25.0‰, respectively, displaying the positive carbon isotopic series. The δ13CCO2 values are generally higher than −8‰, and the δD1 values are from −156‰ to −107‰. The identification of gas origin and gas–source correlation indicate that the natural gases reservoired in the Changxing-Feixianguan Formations in the Yuanba Gas Field have been altered by thermochemical sulfate reduction, and they are mainly composed of oil cracking gases derived from the secondary cracking of oil generated by the sapropelic-prone source rocks in the Permian Longtan Formation. The CO2 in the gas pools are mainly inorganic and were derived from the interaction between the acidic fluid and carbonate reservoirs