Utilisation de la photogrammétrie pour l’étude de la végétation riveraine

International audienceThis study presents the results of methodological tests in aerial photogrammetry with the objective of a diachronic survey of riparian vegetation in three dimensions, i.e. its spatial distribution and height. The study is undertaken at two complementary scales. The first one corresponds to a 10 km reach of the Allier river floodplain. At this scale, aerial photographs were taken from a small airplane. The second level of analysis corresponds to a wooded point bar located within the 10 km reach. At this scale, the photographs were taken from an unmanned aerial vehicle (UAV). Canopy height models (CHM) are produced at two scales of analysis. The accuracy of the CHMs is then analysed by comparison with vegetation height measurements collected during a filed campaign. The results show significant agreement for the models derived from two approaches, with an error of one meter at the floodplain scale and few centimetres at the bar scale. This variation in error is mainly due to different photographic resolutions. The two approaches appeared to be complementary. The first one is adequate for mapping vegetation structure at the floodplain scale, the second for mapping vegetation in smaller areas but at a higher resolution.Cette étude présente les résultats de tests méthodologiques réalisés en photogrammétrie aérienne avec pour objectif le suivi diachronique de la végétation riveraine dans ses trois dimensions, c’est-à-dire sa distribution spatiale et sa hauteur. L’étude est menée à deux échelles d’analyse. La première échelle est celle d’un tronçon longitudinal de 10 km de la plaine alluviale de la rivière Allier. À cette échelle de travail, un avion a été utilisé pour les prises de vue aérienne. La deuxième échelle d’analyse est celle d’un banc boisé, localisé dans la zone de 10 km. À cette échelle, la couverture photographique a été faite à l’aide d’un drone. Pour les deux objets d’étude des modèles de hauteur du couvert végétal (MHC) sont produits. La précision des MHC est ensuite vérifiée à partir de la mesure de la hauteur d’arbres sur le terrain. Les résultats révèlent une très bonne précision des modèles pour les deux approches, de l’ordre du mètre à l’échelle du tronçon de 10 km et de quelques centimètres à l’échelle du banc. Cette inégalité s’explique surtout par des résolutions photographiques différentes pour les deux missions. Il ressort de ce travail que les deux approches sont complémentaires. La première permet de cartographier la structure de la végétation à l’échelle de la plaine alluviale, la deuxième permet une cartographie de la végétation à plus haute résolution mais seulement à une échelle plus réduite

Three-dimensional tumor cell growth stimulates autophagic flux and recapitulates chemotherapy resistance

Current preclinical models in tumor biology are limited in their ability to recapitulate relevant (patho-) physiological processes, including autophagy. Three-dimensional (3D) growth cultures have frequently been proposed to overcome the lack of correlation between two-dimensional (2D) monolayer cell cultures and human tumors in preclinical drug testing. Besides 3D growth, it is also advantageous to simulate shear stress, compound flux and removal of metabolites, e.g., via bioreactor systems, through which culture medium is constantly pumped at a flow rate reflecting physiological conditions. Here we show that both static 3D growth and 3D growth within a bioreactor system modulate key hallmarks of cancer cells, including proliferation and cell death as well as macroautophagy, a recycling pathway often activated by highly proliferative tumors to cope with metabolic stress. The autophagyrelated gene expression profiles of 2D-grown cells are substantially different from those of 3D-grown cells and tumor tissue. Autophagy-controlling transcription factors, such as TFEB and FOXO3, are upregulated in tumors, and 3D-grown cells have increased expression compared with cells grown in 2D conditions. Three-dimensional cultures depleted of the autophagy mediators BECN1, ATG5 or ATG7 or the transcription factor FOXO3, are more sensitive to cytotoxic treatment. Accordingly, combining cytotoxic treatment with compounds affecting late autophagic flux, such as chloroquine, renders the 3D-grown cells more susceptible to therapy. Altogether, 3D cultures are a valuable tool to study drug response of tumor cells, as these models more closely mimic tumor (patho-)physiology, including the upregulation of tumor relevant pathways, such as autophagy

Word Processing differences between dyslexic and control children

BACKGROUND: The aim of this study was to investigate brain responses triggered by different wordclasses in dyslexic and control children. The majority of dyslexic children have difficulties to phonologically assemble a word from sublexical parts following grapheme-to-phoneme correspondences. Therefore, we hypothesised that dyslexic children should mainly differ from controls processing low frequent words that are unfamiliar to the reader. METHODS: We presented different wordclasses (high and low frequent words, pseudowords) in a rapid serial visual word (RSVP) design and performed wavelet analysis on the evoked activity. RESULTS: Dyslexic children had lower evoked power amplitudes and a higher spectral frequency for low frequent words compared to control children. No group differences were found for high frequent words and pseudowords. Control children had higher evoked power amplitudes and a lower spectral frequency for low frequent words compared to high frequent words and pseudowords. This pattern was not present in the dyslexic group. CONCLUSION: Dyslexic children differed from control children only in their brain responses to low frequent words while showing no modulated brain activity in response to the three word types. This might support the hypothesis that dyslexic children are selectively impaired reading words that require sublexical processing. However, the lacking differences between word types raise the question if dyslexic children were able to process the words presented in rapid serial fashion in an adequate way. Therefore the present results should only be interpreted as evidence for a specific sublexical processing deficit with caution

26th Annual Computational Neuroscience Meeting (CNS*2017): Part 3 - Meeting Abstracts - Antwerp, Belgium. 15–20 July 2017

This work was produced as part of the activities of FAPESP Research,\ud Disseminations and Innovation Center for Neuromathematics (grant\ud 2013/07699-0, S. Paulo Research Foundation). NLK is supported by a\ud FAPESP postdoctoral fellowship (grant 2016/03855-5). ACR is partially\ud supported by a CNPq fellowship (grant 306251/2014-0)